Overview

  • Product name
    Anti-Interferon beta antibody [7F-D3]
    See all Interferon beta primary antibodies
  • Description
    Rat monoclonal [7F-D3] to Interferon beta
  • Host species
    Rat
  • Specificity
    This antibody cross react with IFN beta, but not alpha or gamma.
  • Tested applications
    Suitable for: WB, ELISA, Flow Cyt, Neutralisingmore details
  • Species reactivity
    Reacts with: Mouse
    Does not react with: Human
  • Immunogen

    Purified mouse interferon beta.

Properties

Applications

Our Abpromise guarantee covers the use of ab24324 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.
Flow Cyt Use a concentration of 10 µg/ml. PubMed: 24474800

ab18407 - Rat monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

Neutralising Use at an assay dependent concentration. At a concentration of 3.6 ng/ml, this antibody neutralizes 10 U/ml of mouse IFN-b to 1 U/ml.

Target

  • Function
    Has antiviral, antibacterial and anticancer activities.
  • Sequence similarities
    Belongs to the alpha/beta interferon family.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • beta-interferon antibody
    • Fibroblast interferon antibody
    • IFB antibody
    • IFF antibody
    • IFN beta antibody
    • IFN-beta antibody
    • IFNB 1 antibody
    • IFNB antibody
    • IFNB_HUMAN antibody
    • IFNB1 antibody
    • Interferon beta 1 fibroblast antibody
    • Interferon beta antibody
    • Interferon beta precursor antibody
    • MGC96956 antibody
    see all

References

This product has been referenced in:
  • Li Q  et al. cGAS/STING/TBK1/IRF3 Signaling Pathway Activates BMDCs Maturation Following Mycobacterium bovis Infection. Int J Mol Sci 20:N/A (2019). Read more (PubMed: 30791397) »
  • Kerur N  et al. cGAS drives noncanonical-inflammasome activation in age-related macular degeneration. Nat Med 24:50-61 (2018). Read more (PubMed: 29176737) »
See all 12 Publications for this product

Customer reviews and Q&As

Filter by Application

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1-2 of 2 Abreviews

Application
Western blot
Sample
Mouse Recombinant protein (recombinant IFNbeta AND mouse hepatocyte lysate)
Loading amount
0.5 µg
Specification
recombinant IFNbeta AND mouse hepatocyte lysate
Gel Running Conditions
Reduced Denaturing (10% SDS-PAGE)
Blocking step
50:50 PBS:Odyssey Blocking Buffer, Li-Cor 927-40100 as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 50% · Temperature: 24°C

Abcam user community

Verified customer

Submitted Aug 28 2012

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (eye tissue)
Specification
eye tissue
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated
Permeabilization
No

Abcam user community

Verified customer

Submitted Apr 20 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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