Key features and details
- Rabbit polyclonal to Interferon beta
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-Interferon beta antibody
See all Interferon beta primary antibodies
DescriptionRabbit polyclonal to Interferon beta
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Macaque monkey
Synthetic peptide corresponding to Human Interferon beta aa 1-100 conjugated to keyhole limpet haemocyanin.
- Recombinant human Interferon beta protein (ab71475) can be used as a positive control in WB. This antibody gave a positive signal in HepG2 and WI38 whole cell lysates and in the following human tissue lysates: Liver; Lung; Kidney; Spleen.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab85803 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 22 kDa).|
|ICC/IF||Use a concentration of 1 µg/ml.|
FunctionHas antiviral, antibacterial and anticancer activities.
Sequence similaritiesBelongs to the alpha/beta interferon family.
- Information by UniProt
- beta-interferon antibody
- Fibroblast interferon antibody
- IFB antibody
All lanes : Anti-Interferon beta antibody (ab85803) at 1 µg/ml
Lane 1 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 2 :
WI-38 whole cell lysate (ab3960)
Lane 3 : Human liver tissue lysate - total protein (ab29889)
Lane 4 : Lung (Human) Tissue Lysate
Lane 5 : Human kidney tissue lysate - total protein (ab30203)
Lane 6 : Human spleen tissue lysate - total protein (ab29699)
Lysates/proteins at 10 µg per lane.
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 22 kDa
Observed band size: 30 kDa why is the actual band size different from the predicted?
Additional bands at: 110 kDa, 120 kDa, 160 kDa, 37 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 20 minutes
The Interferon Beta protein sequence contains both a phosphorylation and a glycosylation site (Swissprot). Post-translation modifications could account for the migration of the band at a higher molecular weight than predicted.
ICC/IF image of ab85803 stained HepG2 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab85803, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
ab85803 has been referenced in 1 publication.
- Oishi I et al. Efficient production of human interferon beta in the white of eggs from ovalbumin gene-targeted hens. Sci Rep 8:10203 (2018). PubMed: 29976933