Product nameAnti-Interferon beta antibody
See all Interferon beta primary antibodies
DescriptionRabbit polyclonal to Interferon beta
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Macaque monkey
- Recombinant human Interferon beta protein (ab71475) can be used as a positive control in WB. This antibody gave a positive signal in HepG2 and WI38 whole cell lysates and in the following human tissue lysates: Liver; Lung; Kidney; Spleen.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab85803 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 22 kDa).|
|ICC/IF||Use a concentration of 1 µg/ml.|
FunctionHas antiviral, antibacterial and anticancer activities.
Sequence similaritiesBelongs to the alpha/beta interferon family.
- Information by UniProt
- beta-interferon antibody
- Fibroblast interferon antibody
- IFB antibody
All lanes : Anti-Interferon beta antibody (ab85803) at 1 µg/ml
Lane 1 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 2 :
WI-38 whole cell lysate (ab3960)
Lane 3 : Human liver tissue lysate - total protein (ab29889)
Lane 4 : Lung (Human) Tissue Lysate
Lane 5 : Human kidney tissue lysate - total protein (ab30203)
Lane 6 : Human spleen tissue lysate - total protein (ab29699)
Lysates/proteins at 10 µg per lane.
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 22 kDa
Observed band size: 30 kDa why is the actual band size different from the predicted?
Additional bands at: 110 kDa, 120 kDa, 160 kDa, 37 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 20 minutes
The Interferon Beta protein sequence contains both a phosphorylation and a glycosylation site (Swissprot). Post-translation modifications could account for the migration of the band at a higher molecular weight than predicted.
ICC/IF image of ab85803 stained HepG2 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab85803, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.