Question (56637) | Anti-Interferon beta antibody (Biotin) (ab84258)

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Question

Anti-Interferon beta antibody (Biotin) (ab84258). I noticed the website says it reacts with human IFN. Is there any reason why it shouldn't work to detect other species of IFN as well? I want to do immunofluorescence microscopy for mouse IFN in hepatocytes in culture. Second question: The website IF photo description mentions a secondary antibody. Is it possible to use a fluorophore-conjugated streptavidin instead?

Answer

Thank you very much for your interest in ab84258.

Unfortunately the sequence homology between he human and mouse forms of IFNB is very low (47%). We would not be confident that this product would work in mouse.

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