For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome
That sounds like a pretty good offer but my biggest concern is that since we don't know if it works, and if I try it but I don't see any signal in my cells, I won't know if that's because (a) it doesn't work for IF or because (b) my cells aren't making IFN. In other words I don't have a positive control. (I'm treating the hepatocytes with virus, and the literature says that virus-infected hepatocytes make IFN, but I don't really know whether these particular hepatocytes really do it. Basically I was hoping to test them and see if they do this). So, I am not sure if I will be able to write a useful review. If you have any suggestions about this I would be interested. Thanks,
Asked on Aug 03 2012
Thank you for your reply.
I do understand your concerns. It may be that you would want to run a western blot with this product to ensure that it is picking up IFN from your cells, it would them be possible to run a purified protein as control since we are unsure the hepatocytes would express. If this worked you could then stain with more confidence.
I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.
Use our products? Submit an Abreview. Earn rewards!
Answered on Aug 03 2012