Question (56647) | Anti-Interferon beta antibody (Biotin) (ab84258)

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Question

That sounds like a pretty good offer but my biggest concern is that since we don't know if it works, and if I try it but I don't see any signal in my cells, I won't know if that's because (a) it doesn't work for IF or because (b) my cells aren't making IFN. In other words I don't have a positive control. (I'm treating the hepatocytes with virus, and the literature says that virus-infected hepatocytes make IFN, but I don't really know whether these particular hepatocytes really do it. Basically I was hoping to test them and see if they do this). So, I am not sure if I will be able to write a useful review. If you have any suggestions about this I would be interested. Thanks,

Answer

Thank you for your reply.

I do understand your concerns. It may be that you would want to run a western blot with this product to ensure that it is picking up IFN from your cells, it would them be possible to run a purified protein as control since we are unsure the hepatocytes would express. If this worked you could then stain with more confidence.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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