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    interferon-beta-antibody-epr22186-266-ab218229.pdf

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RecombinantRabMAb

Recombinant Anti-Interferon beta antibody [EPR22186-266] (ab218229)

  • Datasheet
  • Certificate of Compliance
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Immunoprecipitation - Anti-Interferon beta antibody [EPR22186-266] (ab218229)
  • Western blot - Anti-Interferon beta antibody [EPR22186-266] (ab218229)
  • Anti-Interferon beta antibody [EPR22186-266] (ab218229)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22186-266] to Interferon beta
  • Suitable for: IP, WB
  • Reacts with: Mouse

Conjugates logo Related conjugates and formulations

Carrier Free

You may also be interested in

Primary
Product image
Anti-GADD45A antibody (ab180768)
Secondary
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Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
Protein
Recombinant human Interferon beta protein (Active) (ab71475)

View more associated products

Overview

  • Product name

    Anti-Interferon beta antibody [EPR22186-266]
    See all Interferon beta primary antibodies
  • Description

    Rabbit monoclonal [EPR22186-266] to Interferon beta
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IP, WBmore details
  • Species reactivity

    Reacts with: Mouse
  • Immunogen

    Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: RAW 264.7 treated with with 50 µg/ml DMXAA (ab143018) for 7 hours, then with 300 ng/ml Brefeldin A (BFA) added after 1 hour, whole cell lysate; RAW 264.7 treated with 100 ng/ml lipopolysaccharide (LPS) for 6 hours, then with 300 ng/ml Brefeldin A (BFA) added after 3 hours, whole cell lysate. IP: RAW 264.7 treated with 50µg/ml DMXAA (ab143018) for 1h, then together with 300ng/ml brefeldin A (BFA) for another 6h, whole cell lysate.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR22186-266
  • Isotype

    IgG
  • Research areas

    • Cardiovascular
    • Angiogenesis
    • Cytokines
    • Other
    • Immunology
    • Innate Immunity
    • Cytokines
    • Interferons
    • Cancer
    • Tumor immunology
    • Cytokines
    • Interferons
    • Neuroscience
    • Diseases
    • Neuroscience
    • Processes

Associated products

  • Alternative Versions

    • Anti-Interferon beta antibody [EPR22186-266] - BSA and Azide free (ab238652)
  • Compatible Secondaries

    • VeriBlot for IP Detection Reagent (HRP) (ab131366)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Recombinant Protein

    • Recombinant human Interferon beta protein (Active) (ab71475)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab218229 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP
1/30.
WB
1/1000. Detects a band of approximately 24, 26 kDa (predicted molecular weight: 22 kDa).
Notes
IP
1/30.
WB
1/1000. Detects a band of approximately 24, 26 kDa (predicted molecular weight: 22 kDa).

Target

  • Function

    Has antiviral, antibacterial and anticancer activities.
  • Sequence similarities

    Belongs to the alpha/beta interferon family.
  • Cellular localization

    Secreted.
  • Target information above from: UniProt accession P01574 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 15977 Mouse
    • SwissProt: P01575 Mouse
    • Unigene: 1245 Mouse
    • Alternative names

      • beta-interferon antibody
      • Fibroblast interferon antibody
      • IFB antibody
      • IFF antibody
      • IFN beta antibody
      • IFN-beta antibody
      • IFNB 1 antibody
      • IFNB antibody
      • IFNB_HUMAN antibody
      • IFNB1 antibody
      • Interferon beta 1 fibroblast antibody
      • Interferon beta antibody
      • Interferon beta precursor antibody
      • MGC96956 antibody
      see all

    Images

    • Immunoprecipitation - Anti-Interferon beta antibody [EPR22186-266] (ab218229)
      Immunoprecipitation - Anti-Interferon beta antibody [EPR22186-266] (ab218229)

      Interferon beta was immunoprecipitated from 0.35 mg of RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) treated with 50μg/ml DMXAA (ab143018) for 1h, then together with 300ng/ml brefeldin A (BFA) for another 6h whole cell lysate with ab218229 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab218229 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

      Lane 1: RAW 264.7 (treated with DMXAA (ab143018)/BFA) whole cell lysate 10 μg (Input).
      Lane 2: ab218229 IP in RAW 264.7 (treated with DMXAA (ab143018)/BFA) whole cell lysate.
      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab218229 in RAW 264.7 (treated with DMXAA (ab143018)/BFA) whole cell lysate.

      Blocking and dilution buffer: 5% NFDM/TBST.

    • Western blot - Anti-Interferon beta antibody [EPR22186-266] (ab218229)
      Western blot - Anti-Interferon beta antibody [EPR22186-266] (ab218229)
      All lanes : Anti-Interferon beta antibody [EPR22186-266] (ab218229) at 1/1000 dilution

      Lane 1 : Untreated RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
      Lane 2 : RAW 264.7 treated with 50 µg/ml DMXAA (ab143018) for 7 hours, then with 300 ng/ml Brefeldin A (BFA) added after 1 hour, whole cell lysate
      Lane 3 : Untreated RAW 264.7 whole cell lysate
      Lane 4 : RAW264.7 treated with 100 ng/ml lipopolysaccharide (LPS) for 6 hours, then with 300 ng/ml Brefeldin A (BFA) added after 3 hours, whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 22 kDa
      Observed band size: 24,26 kDa why is the actual band size different from the predicted?



      Exposure time : Lanes 1 and 2: 26 seconds; Lanes 3 and 4: 3 minutes.

      Blocking/Dilution buffer: 5% NFDM/TBST.

      Interferon beta can be induced to increase the expression by DMXAA or LPS in RAW264.7 cell (PMID: 23027866, PMID: 16020513).

      The doublet is likely due to isoforms (PMID: 7352261).

    • Anti-Interferon beta antibody [EPR22186-266] (ab218229)
      Anti-Interferon beta antibody [EPR22186-266] (ab218229)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet download

      Download

    Certificate of Compliance

    To download a Certificate of Compliance, please enter your Lot number below:

    References (0)

    Publishing research using ab218229? Please let us know so that we can cite the reference in this datasheet.

    ab218229 has not yet been referenced specifically in any publications.

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