Recombinant Anti-Interferon beta antibody [EPR22186-266] (ab218229)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22186-266] to Interferon beta
- Suitable for: IP, WB
- Reacts with: Mouse
Overview
-
Product name
Anti-Interferon beta antibody [EPR22186-266]
See all Interferon beta primary antibodies -
Description
Rabbit monoclonal [EPR22186-266] to Interferon beta -
Host species
Rabbit -
Tested applications
Suitable for: IP, WBmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Recombinant full length protein within Mouse Interferon beta aa 1 to the C-terminus. The exact sequence is proprietary.
Database link: P01575 -
Positive control
- WB: RAW 264.7 treated with with 50 µg/ml DMXAA (ab143018) for 7 hours, then with 300 ng/ml Brefeldin A (BFA) added after 1 hour, whole cell lysate; RAW 264.7 treated with 100 ng/ml lipopolysaccharide (LPS) for 6 hours, then with 300 ng/ml Brefeldin A (BFA) added after 3 hours, whole cell lysate. IP: RAW 264.7 treated with 50µg/ml DMXAA (ab143018) for 1h, then together with 300ng/ml brefeldin A (BFA) for another 6h, whole cell lysate.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR22186-266 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
-
Related Products
Applications
Our Abpromise guarantee covers the use of ab218229 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IP | 1/30. | |
| WB | 1/1000. Detects a band of approximately 24, 26 kDa (predicted molecular weight: 22 kDa). |
Target
-
Function
Has antiviral, antibacterial and anticancer activities. -
Sequence similarities
Belongs to the alpha/beta interferon family. -
Cellular localization
Secreted. - Information by UniProt
-
Database links
- Entrez Gene: 15977 Mouse
- SwissProt: P01575 Mouse
- Unigene: 1245 Mouse
-
Alternative names
- beta-interferon antibody
- Fibroblast interferon antibody
- IFB antibody
see all
Images
-
![Immunoprecipitation - Anti-Interferon beta antibody [EPR22186-266] (ab218229)](https://www.abcam.com/ps/products/218/ab218229/Images/ab218229-316590-anti-interferon-beta-antibody-epr22186-266-immunoprecipitation.jpg)
Immunoprecipitation - Anti-Interferon beta antibody [EPR22186-266] (ab218229)Interferon beta was immunoprecipitated from 0.35 mg of RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) treated with 50μg/ml DMXAA (ab143018) for 1h, then together with 300ng/ml brefeldin A (BFA) for another 6h whole cell lysate with ab218229 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab218229 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.
Lane 1: RAW 264.7 (treated with DMXAA (ab143018)/BFA) whole cell lysate 10 μg (Input).
Lane 2: ab218229 IP in RAW 264.7 (treated with DMXAA (ab143018)/BFA) whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab218229 in RAW 264.7 (treated with DMXAA (ab143018)/BFA) whole cell lysate.Blocking and dilution buffer: 5% NFDM/TBST.
-
![Western blot - Anti-Interferon beta antibody [EPR22186-266] (ab218229)](https://www.abcam.com/ps/products/218/ab218229/Images/ab218229-316589-anti-interferon-beta-antibody-epr22186-266-western-blot.jpg)
Western blot - Anti-Interferon beta antibody [EPR22186-266] (ab218229)All lanes : Anti-Interferon beta antibody [EPR22186-266] (ab218229) at 1/1000 dilution
Lane 1 : Untreated RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
Lane 2 : RAW 264.7 treated with 50 µg/ml DMXAA (ab143018) for 7 hours, then with 300 ng/ml Brefeldin A (BFA) added after 1 hour, whole cell lysate
Lane 3 : Untreated RAW 264.7 whole cell lysate
Lane 4 : RAW264.7 treated with 100 ng/ml lipopolysaccharide (LPS) for 6 hours, then with 300 ng/ml Brefeldin A (BFA) added after 3 hours, whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 22 kDa
Observed band size: 24,26 kDa why is the actual band size different from the predicted?Exposure time : Lanes 1 and 2: 26 seconds; Lanes 3 and 4: 3 minutes.
Blocking/Dilution buffer: 5% NFDM/TBST.
Interferon beta can be induced to increase the expression by DMXAA or LPS in RAW264.7 cell (PMID: 23027866, PMID: 16020513).
The doublet is likely due to isoforms (PMID: 7352261).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
Certificate of Compliance
References (0)
ab218229 has not yet been referenced specifically in any publications.
