Recombinant
RabMAb

Recombinant Anti-Interferon beta antibody [EPR22186-266] (ab218229)

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Overview

  • Product name

    Anti-Interferon beta antibody [EPR22186-266]
    See all Interferon beta primary antibodies

  • Description

    Rabbit monoclonal [EPR22186-266] to Interferon beta

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IP, WBmore details

  • Species reactivity

    Reacts with: Mouse

  • Immunogen

    Recombinant full length protein within Mouse Interferon beta aa 1 to the C-terminus. The exact sequence is proprietary.
    Database link: P01575

  • Positive control

    • WB: RAW 264.7 treated with with 50 µg/ml DMXAA (ab143018) for 7 hours, then with 300 ng/ml Brefeldin A (BFA) added after 1 hour, whole cell lysate; RAW 264.7 treated with 100 ng/ml lipopolysaccharide (LPS) for 6 hours, then with 300 ng/ml Brefeldin A (BFA) added after 3 hours, whole cell lysate. IP: RAW 264.7 treated with 50µg/ml DMXAA (ab143018) for 1h, then together with 300ng/ml brefeldin A (BFA) for another 6h, whole cell lysate.

  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab218229 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP 1/30.
WB 1/1000. Detects a band of approximately 24, 26 kDa (predicted molecular weight: 22 kDa).

Target

  • Function

    Has antiviral, antibacterial and anticancer activities.

  • Sequence similarities

    Belongs to the alpha/beta interferon family.

  • Cellular localization

    Secreted.
  • Information by UniProt

  • Database links

    • Alternative names

      • beta-interferon antibody
      • Fibroblast interferon antibody
      • IFB antibody
      • IFF antibody
      • IFN beta antibody
      • IFN-beta antibody
      • IFNB 1 antibody
      • IFNB antibody
      • IFNB_HUMAN antibody
      • IFNB1 antibody
      • Interferon beta 1 fibroblast antibody
      • Interferon beta antibody
      • Interferon beta precursor antibody
      • MGC96956 antibody
      see all

    Images

    • Immunoprecipitation - Anti-Interferon beta antibody [EPR22186-266] (ab218229)
      Immunoprecipitation - Anti-Interferon beta antibody [EPR22186-266] (ab218229)

      Interferon beta was immunoprecipitated from 0.35 mg of RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) treated with 50μg/ml DMXAA (ab143018) for 1h, then together with 300ng/ml brefeldin A (BFA) for another 6h whole cell lysate with ab218229 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab218229 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

      Lane 1: RAW 264.7 (treated with DMXAA (ab143018)/BFA) whole cell lysate 10 μg (Input).
      Lane 2: ab218229 IP in RAW 264.7 (treated with DMXAA (ab143018)/BFA) whole cell lysate.
      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab218229 in RAW 264.7 (treated with DMXAA (ab143018)/BFA) whole cell lysate.

      Blocking and dilution buffer: 5% NFDM/TBST.

    • Western blot - Anti-Interferon beta antibody [EPR22186-266] (ab218229)
      Western blot - Anti-Interferon beta antibody [EPR22186-266] (ab218229)
      All lanes : Anti-Interferon beta antibody [EPR22186-266] (ab218229) at 1/1000 dilution

      Lane 1 : Untreated RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
      Lane 2 : RAW 264.7 treated with 50 µg/ml DMXAA (ab143018) for 7 hours, then with 300 ng/ml Brefeldin A (BFA) added after 1 hour, whole cell lysate
      Lane 3 : Untreated RAW 264.7 whole cell lysate
      Lane 4 : RAW264.7 treated with 100 ng/ml lipopolysaccharide (LPS) for 6 hours, then with 300 ng/ml Brefeldin A (BFA) added after 3 hours, whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 22 kDa
      Observed band size: 24,26 kDa why is the actual band size different from the predicted?



      Exposure time : Lanes 1 and 2: 26 seconds; Lanes 3 and 4: 3 minutes.

      Blocking/Dilution buffer: 5% NFDM/TBST.

      Interferon beta can be induced to increase the expression by DMXAA or LPS in RAW264.7 cell (PMID: 23027866, PMID: 16020513).

      The doublet is likely due to isoforms (PMID: 7352261).

    References

    ab218229 has not yet been referenced specifically in any publications.

    Publishing research using ab218229? Please let us know so that we can cite the reference in this datasheet.

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