Key features and details
- Rabbit polyclonal to Interferon gamma
- Suitable for: WB
- Isotype: IgG
Product nameAnti-Interferon gamma antibody
See all Interferon gamma primary antibodies
DescriptionRabbit polyclonal to Interferon gamma
Tested applicationsSuitable for: WBmore details
Predicted to work with: Sheep, Goat, Cow
- WB: Recombinant Cow Interferon gamma protein.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: 50% Glycerol (glycerin, glycerine), PBS, 0.03% Proclin 300
Concentration information loading...
PurityProtein G purified
Purification notesPurity >95%.
Our Abpromise guarantee covers the use of ab230002 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/5000. Detects a band of approximately 20 kDa (predicted molecular weight: 19 kDa).|
FunctionProduced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons.
Tissue specificityReleased primarily from activated T lymphocytes.
Involvement in diseaseIn Caucasians, genetic variation in IFNG is associated with the risk of aplastic anemia (AA) [MIM:609135]. AA is a rare disease in which the reduction of the circulating blood cells results from damage to the stem cell pool in bone marrow. In most patients, the stem cell lesion is caused by an autoimmune attack. T-lymphocytes, activated by an endogenous or exogenous, and most often unknown antigenic stimulus, secrete cytokines, including IFN-gamma, which would in turn be able to suppress hematopoiesis.
Sequence similaritiesBelongs to the type II (or gamma) interferon family.
modificationsProteolytic processing produces C-terminal heterogeneity, with proteins ending alternatively at Gly-150, Met-157 or Gly-161.
- Information by UniProt
- IF 1 antibody
- IFG antibody
- IFI antibody
All lanes : Anti-Interferon gamma antibody (ab230002) at 1/500 dilution
Lane 1 : Recombinant Cow Interferon gamma protein at 0.2 µg
Lane 2 : Recombinant Cow Interferon gamma protein at 0.1 µg
Lane 3 : Recombinant Cow Interferon gamma protein at 0.05 µg
All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 19 kDa
Observed band size: 20 kDa why is the actual band size different from the predicted?
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab230002 has not yet been referenced specifically in any publications.