Anti-Interferon gamma antibody (ab230002)
Key features and details
- Rabbit polyclonal to Interferon gamma
- Suitable for: WB
- Reacts with: Recombinant fragment
- Isotype: IgG
Overview
-
Product name
Anti-Interferon gamma antibody
See all Interferon gamma primary antibodies -
Description
Rabbit polyclonal to Interferon gamma -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Recombinant fragment
Predicted to work with: Sheep, Goat -
Immunogen
Recombinant full length protein corresponding to Cow Interferon gamma aa 1 to the C-terminus.
Database link: P07353 -
Positive control
- WB: Recombinant Cow Interferon gamma protein.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
-
Purity
Protein G purified -
Purification notes
Purity >95%. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Isotype control
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab230002 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/500 - 1/5000. Detects a band of approximately 20 kDa (predicted molecular weight: 19 kDa).
|
Notes |
---|
WB
1/500 - 1/5000. Detects a band of approximately 20 kDa (predicted molecular weight: 19 kDa). |
Target
-
Function
Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons. -
Tissue specificity
Released primarily from activated T lymphocytes. -
Involvement in disease
In Caucasians, genetic variation in IFNG is associated with the risk of aplastic anemia (AA) [MIM:609135]. AA is a rare disease in which the reduction of the circulating blood cells results from damage to the stem cell pool in bone marrow. In most patients, the stem cell lesion is caused by an autoimmune attack. T-lymphocytes, activated by an endogenous or exogenous, and most often unknown antigenic stimulus, secrete cytokines, including IFN-gamma, which would in turn be able to suppress hematopoiesis. -
Sequence similarities
Belongs to the type II (or gamma) interferon family. -
Post-translational
modificationsProteolytic processing produces C-terminal heterogeneity, with proteins ending alternatively at Gly-150, Met-157 or Gly-161. -
Cellular localization
Secreted. - Information by UniProt
-
Database links
- Entrez Gene: 100860815 Goat
- Entrez Gene: 443396 Sheep
- SwissProt: P79154 Goat
- SwissProt: P17773 Sheep
-
Alternative names
- IF 1 antibody
- IFG antibody
- IFI antibody
see all
Images
-
All lanes : Anti-Interferon gamma antibody (ab230002) at 1/500 dilution
Lane 1 : Recombinant Cow Interferon gamma protein at 0.2 µg
Lane 2 : Recombinant Cow Interferon gamma protein at 0.1 µg
Lane 3 : Recombinant Cow Interferon gamma protein at 0.05 µg
Secondary
All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 19 kDa
Observed band size: 20 kDa why is the actual band size different from the predicted?
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
SDS download
-
Datasheet download
References (0)
ab230002 has not yet been referenced specifically in any publications.