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I was looking for a monoclonal antibody against human IFN y, to be used in sandwich ELISA. We already have an antibody against this target, and having a second antibody would of course enable us to potentially set up a sandwich ELISA. I noticed several antibodies on your website that could be interesting, but unfortunately, the epitope targeted is not stated. Do you maybe have an antibody with our desired specifications and a known epitope?
Asked on Mar 29 2012
Thank you for your interest in our IFN gamma antibodies.
I recommend using your monoclonal antibody for capture in a sandwich ELISA, and a rabbit anti-human IFN gamma polyclonal antibody raised against full-length protein for detection, followed by an anti-rabbit IgG secondary antibody, pre-adsorbed against mouse IgG to remove reactivity with mouse (assuming your monoclonal antibody is mouse IgG). The secondary will be conjugated to HRP or AP or fluorochrome, depending on how you anticipate detecting the signal.
I suggest ab25101 for the polyclonal IFN gamma antibody,
Click here (or use the following: https://www.abcam.com/index.html?datasheet=25101)
and I suggest ab97080 for the anti-rabbit secondary, assuming you use HRP plus substrate for detection.
Click here (or use the following: https://www.abcam.com/index.html?datasheet=97080).
The epitopes the antibody recognizes are not mapped, since this is a polyclonal antibody which recognizes multiple epitopes along the entire length of IFN gamma. If you want a monoclonal antibody (which recognizes a single epitope, rather than many) to pair with your monoclonal, I will need to know the epitope that your antibody recognizes in order to select an appropriate match. One of the two mouse monoclonal antibodies will need to be directly conjugated to an enzyme or fluorochrome, to avoid using an anti-mouse IgG secondary antibody.
I hope this is helpful. Please let me know if you have questions.
Answered on Mar 29 2012