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As you know we have ordered a number of anti-human ab from Abcam. The list
I would like to request fro your assistance in the following...
We would like to get the information about the recommended diluent for the
ab to be used for IHC with paraffin embedded tissue sections since its known
to affect results at times.
Other information regarding antigen retrieval conditions etc will be
available on the net, but could be refurbished if they are updated.
Equally important is the source of positive and negative controls.
I would especially like to request for slides of both the types of controls
as they are vital to decide/judge the variations in the protocols. I hope
this is done as it will serve us for a long time in future.
Asked on May 11 2012
Thank you for contacting us.
I have attached the filled out Exel spreadsheet with all the requested information. To be fair, all the information asked could be answered with a look on the datasheet...
In addition, I had a look through our tissue slices for her IHC-P experiments. Instead of the Tonsils she can use Lymphknot (as we do not have Tonsil slides).
https://www.abcam.com/index.html?datasheet=4350 (or use the following: https://www.abcam.com/index.html?datasheet=4350). PC for ab25101and ab10563
https://www.abcam.com/index.html?datasheet=4348 (or use the following: https://www.abcam.com/index.html?datasheet=4348). PC for ab34843
https://www.abcam.com/index.html?datasheet=4372 (or use the following: https://www.abcam.com/index.html?datasheet=4372). PC for ab37158
Answered on May 11 2012