- Datasheet
- Specific References (1)
- Protocols
Overview
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Product nameAnti-Interferon gamma antibody
See all Interferon gamma primary antibodies -
DescriptionRabbit polyclonal to Interferon gamma
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Host speciesRabbit
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Tested applicationsSuitable for: Sandwich ELISA, WB, ELISA, Neutralisingmore details
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Species reactivityReacts with: Mouse
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Immunogen
Highly pure (>98%) recombinant mIFN-g (mouse Interferon-gamma).
Properties
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FormLyophilised:Reconstitute with 200µl of sterile water. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
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Storage bufferPBS, pH 7.4, no preservative, sterile filtered
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Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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Isotypeunknown
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Light chain typeunknown
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Research areas
Associated products
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Compatible Secondaries
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Positive Controls
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sELISA pair antibody
Applications
Our Abpromise guarantee covers the use of ab9918 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Sandwich ELISA | Use a concentration of 0.5 - 2 µg/ml. Can be paired for Sandwich ELISA with Rabbit polyclonal to Interferon gamma (Biotin) (ab83136). This antigen affinity purified antibody, in conjunction with recommended pair as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mIFN-gamma. Can be used as capture antibody when paired with ab83136. | |
| WB | Use a concentration of 0.1 - 0.2 µg/ml. | |
| ELISA | Use a concentration of 0.5 µg/ml. Allows the detection of 0.2 - 0.4 ng/well of recombinant mIFN-g. | |
| Neutralising | Use a concentration of 0.004 - 0.006 µg/ml. for one-half maximal inhibition [ND50] of the biological activity of mIFN-g (50 pg/ml). |
Target
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FunctionProduced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons.
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Tissue specificityReleased primarily from activated T lymphocytes.
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Involvement in diseaseIn Caucasians, genetic variation in IFNG is associated with the risk of aplastic anemia (AA) [MIM:609135]. AA is a rare disease in which the reduction of the circulating blood cells results from damage to the stem cell pool in bone marrow. In most patients, the stem cell lesion is caused by an autoimmune attack. T-lymphocytes, activated by an endogenous or exogenous, and most often unknown antigenic stimulus, secrete cytokines, including IFN-gamma, which would in turn be able to suppress hematopoiesis.
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Sequence similaritiesBelongs to the type II (or gamma) interferon family.
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Post-translational
modificationsProteolytic processing produces C-terminal heterogeneity, with proteins ending alternatively at Gly-150, Met-157 or Gly-161. -
Cellular localizationSecreted.
- Information by UniProt
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Database links
- Entrez Gene: 15978 Mouse
- SwissProt: P01580 Mouse
- Unigene: 240327 Mouse
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Alternative names
- IFG antibody
- IFI antibody
- IFN gamma antibody
see all
Protocols
References
This product has been referenced in:
- Mastronardi FG et al. Synergy between paclitaxel plus an exogenous methyl donor in the suppression of murine demyelinating diseases. Mult Scler 13:596-609 (2007). Read more (PubMed: 17548438) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"