Overview

  • Product name
    Anti-Interferon gamma antibody
    See all Interferon gamma primary antibodies
  • Description
    Rabbit polyclonal to Interferon gamma
  • Host species
    Rabbit
  • Tested applications
    Suitable for: Sandwich ELISA, WB, ELISA, Neutralisingmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Highly pure (>98%) recombinant mIFN-g (mouse Interferon-gamma).

  • Positive control
    • Recombinant mouse Interferon gamma protein (ab9922) can be used as a positive control in WB.

Properties

Applications

Our Abpromise guarantee covers the use of ab9918 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use a concentration of 0.5 - 2 µg/ml. Can be paired for Sandwich ELISA with Rabbit polyclonal to Interferon gamma (Biotin) (ab83136). This antigen affinity purified antibody, in conjunction with recommended pair as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mIFN-gamma. Can be used as capture antibody when paired with ab83136.
WB Use a concentration of 0.1 - 0.2 µg/ml.
ELISA Use a concentration of 0.5 µg/ml. Allows the detection of 0.2 - 0.4 ng/well of recombinant mIFN-g.
Neutralising Use a concentration of 0.004 - 0.006 µg/ml. for one-half maximal inhibition [ND50] of the biological activity of mIFN-g (50 pg/ml).

Target

  • Function
    Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons.
  • Tissue specificity
    Released primarily from activated T lymphocytes.
  • Involvement in disease
    In Caucasians, genetic variation in IFNG is associated with the risk of aplastic anemia (AA) [MIM:609135]. AA is a rare disease in which the reduction of the circulating blood cells results from damage to the stem cell pool in bone marrow. In most patients, the stem cell lesion is caused by an autoimmune attack. T-lymphocytes, activated by an endogenous or exogenous, and most often unknown antigenic stimulus, secrete cytokines, including IFN-gamma, which would in turn be able to suppress hematopoiesis.
  • Sequence similarities
    Belongs to the type II (or gamma) interferon family.
  • Post-translational
    modifications
    Proteolytic processing produces C-terminal heterogeneity, with proteins ending alternatively at Gly-150, Met-157 or Gly-161.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • IFG antibody
    • IFI antibody
    • IFN gamma antibody
    • IFN, immune antibody
    • IFN-gamma antibody
    • IFNG antibody
    • IFNG_HUMAN antibody
    • Immune interferon antibody
    • Interferon gamma antibody
    see all

References

This product has been referenced in:
  • Mastronardi FG  et al. Synergy between paclitaxel plus an exogenous methyl donor in the suppression of murine demyelinating diseases. Mult Scler 13:596-609 (2007). Read more (PubMed: 17548438) »
See 1 Publication for this product

Customer reviews and Q&As

1-6 of 6 Abreviews or Q&A

Answer

Thank you for contacting us.


I have been in contact with the lab, I can confirm that ab9918 has already been reconstituted in 200ul sterile water. Since this antibody was lyophilised in PBS, this means that upon the addition of the water, the antibody has been re-dissolved in PBS. There is an error on the datasheet concerning the reconstitution in sterile water and we apologize for this and thank you for bringing this to our attention as it will allow us to update our datasheet.

With regards to storage, we have decided to change the storage conditions to -20ºC instead of 4ºC simply so that the antibody’s activity will be stable for longer. As a rough guide, we suggest that an antibody’s shelf life at 4ºC is 1 year and at -20ºC is 5 years. I can reassure you though that there has been no change in the production of this antibody and that it should function exactly the same as before.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Free Rabbit monoclonal antibody with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

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Answer

Thank you for your email.

There may be some antibodies where I might have suggested the human tissues or cells. I apologize for this. I have included the positive control information irrespective of species so similar mouse tissues sections can used as positive control.

Actually the positive control information for each antibody is present on the datasheet; please check the datasheet. Alternatively, we always follow human protein Atlas database (http://www.proteinatlas.org/index.php). The second option is, searching positive control for other antibodies in catalogue against same target; the cell line or tissue sections tested with those antibodies could also be used as positive control. The third place to search for is literature.

I hope this information will be helpful. Should you have any question please do not hesitate to contact me.

Read More

Answer

Thank you for contacting us.

The positive control for ab36392 is IL2 activatedcultured NK cells.

ab9918; human tonsil sections, human cervical squamous cell carcinoma section

ab34674; human artery or colon tissue sections

ab15537; HepG2 cells. mouse brain sections, human placenta sections; Human hepatocellular liver carcinoma cell line; Human hepatocarcinoma tissue sections

ab2557; mouse spleen tissue sections

ab9475; human tonsil tissue sections

ab22378; mouse spleen tissue sections

ab51312; Thymus tissue sections, human pancreas tissue sections.

The best database to search for positive control is Human Protein Atlas. Please click the link http://www.proteinatlas.org/index.php

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
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Answer

Thank you for your reply.

It does look like we changed the storage instructions of ab2557. Since this product was tested in a guaranteed species and technique and she wasn't seeing any staining, we could offer a free of charge replacement order from the original order. Please let me know if that is what the customer would prefer.

For the spotty staining in IHC, it may be that the antibody is binding to the blocking buffer. She should filter the blocking buffer before adding to the slide. Also she should run a no primary control to determine if the secondary is binding to something non-specifically in the slide. Also the tissue maynot washed enough orfixative may still present. And though they used a gentle permeabilization agent, pemeabilization may have damaged the membrane and removed the membrane protein
Remove permeabilizing agent from your buffers.

I hope this information helps. Please let me know what the customer would like to do with the ab2557.

Read More

Answer

Thank you forhaving your customercomplete our questionnaire. I am sorry to hear that these antibodies are not providing satisfactory results for them. The details provided will enable us to investigate this case and will provide us with vital information for monitoring product quality.

There are some issues with the antibodies that may be resulting in the lack of staining. Firstly, the neutrophil antibody ab2557 was stored in the refrigerator. The datasheet for this antibody says to store at -20C. The last order I have from you for this product is in January. It is possible that storing this antibody improperly for 4 months has affected its quality.

Secondly, the NK antibody ab36392 is not tested in mouse, only rat. We do not guarantee this antibody to work in mouse. Does the customer have access to rat tissue to run a positive control? Also this is the antibody that was giving spotty staining. Have they run a no-primary control? Or tried filtering the blocking buffer?

Lastly, the interferon gamma antibody ab9918 is not tested in IHC-P or ICC and is therefore not guaranteed to work in these applications.

Since two of the antibodies are being used in untested ways and the neutrophil antibody was improperly stored, there are many reasons why the customer may not be seeing staining.

I hope this information is helpful, and I thank you for your cooperation.

Read More
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (thumus)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: EDTA(ventana CC1)
Permeabilization
No
Specification
thumus
Fixative
10% neutral formalin

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Submitted May 04 2017

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