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    interferon-gamma-antibody-ab9918.pdf

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Immunology Innate Immunity Cytokines Interferons
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Anti-Interferon gamma antibody (ab9918)

  • Datasheet
  • SDS
Reviews (2)Q&A (5)References (2)

Product price, shipping and contact information

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Key features and details

  • Rabbit polyclonal to Interferon gamma
  • Reacts with: Mouse
  • Isotype: unknown

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Protein
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Recombinant Human Interferon gamma protein (Active) (ab259377)

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Overview

  • Product name

    Anti-Interferon gamma antibody
    See all Interferon gamma primary antibodies
  • Description

    Rabbit polyclonal to Interferon gamma
  • Host species

    Rabbit
  • Species reactivity

    Reacts with: Mouse
  • Immunogen

    Highly pure (>98%) recombinant mIFN-g (mouse Interferon-gamma).

  • Positive control

    • Recombinant mouse Interferon gamma protein (ab9922) can be used as a positive control in WB.

Properties

  • Form

    Lyophilized:Reconstitute with 200µl of sterile water. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary.
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer

    No preservative, sterile filtered
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    unknown
  • Light chain type

    unknown
  • Research areas

    • Immunology
    • Innate Immunity
    • Cytokines
    • Interferons
    • Stem Cells
    • Signaling Pathways
    • TGF beta
    • Interferon gamma
    • Cancer
    • Tumor immunology
    • Cytokines
    • Interferons
    • Immunology
    • Adaptive Immunity
    • Regulatory T Cells

Associated products

  • Positive Controls

    • Recombinant mouse Interferon gamma protein (Active) (ab9922)
  • Recombinant Protein

    • Recombinant Human Interferon gamma protein (Active) (ab259377)
  • sELISA pair antibody

    • Biotin Anti-Interferon gamma antibody (ab83136)

Target

  • Function

    Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons.
  • Tissue specificity

    Released primarily from activated T lymphocytes.
  • Involvement in disease

    In Caucasians, genetic variation in IFNG is associated with the risk of aplastic anemia (AA) [MIM:609135]. AA is a rare disease in which the reduction of the circulating blood cells results from damage to the stem cell pool in bone marrow. In most patients, the stem cell lesion is caused by an autoimmune attack. T-lymphocytes, activated by an endogenous or exogenous, and most often unknown antigenic stimulus, secrete cytokines, including IFN-gamma, which would in turn be able to suppress hematopoiesis.
  • Sequence similarities

    Belongs to the type II (or gamma) interferon family.
  • Post-translational
    modifications

    Proteolytic processing produces C-terminal heterogeneity, with proteins ending alternatively at Gly-150, Met-157 or Gly-161.
  • Cellular localization

    Secreted.
  • Target information above from: UniProt accession P01579 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 15978 Mouse
    • SwissProt: P01580 Mouse
    • Unigene: 240327 Mouse
    • Alternative names

      • IF 1 antibody
      • IFG antibody
      • IFI antibody
      • IFN gamma antibody
      • IFN immune antibody
      • IFN, immune antibody
      • IFN-gamma antibody
      • IFNG antibody
      • IFNG_HUMAN antibody
      • Immune interferon antibody
      • Interferon gamma antibody
      • Type II Interferon antibody
      see all

    Protocols

    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (2)

    Publishing research using ab9918? Please let us know so that we can cite the reference in this datasheet.

    ab9918 has been referenced in 2 publications.

    • Zheng C  et al. Low-intensity pulsed ultrasound attenuates cardiac inflammation of CVB3-induced viral myocarditis via regulation of caveolin-1 and MAPK pathways. J Cell Mol Med 23:1963-1975 (2019). PubMed: 30592150
    • Mastronardi FG  et al. Synergy between paclitaxel plus an exogenous methyl donor in the suppression of murine demyelinating diseases. Mult Scler 13:596-609 (2007). PubMed: 17548438

    Customer reviews and Q&As

    Show All Reviews Q&A
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    1-7 of 7 Abreviews or Q&A

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Interferon gamma antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Dog Tissue sections (Lymph node)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Tris/EDTA
    Permeabilization
    Yes - 3% H2O2
    Specification
    Lymph node
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Feb 27 2019

    Question

    Dear Sir / Madam,

    We ordered the anti-interferon gamma antibody (ab9918) and received it as a solution. According to the datasheet it is lyophilised and has to be reconstituted with 200 ul of sterile water; whereas the storage buffer is said to be PBS. So could you tell me whether I already received it in PBS?

    We ordered this antibody previously and then it could be stored at 4C when is was dissolved in PBS (according to the datasheet), whereas the datasheet now tells to aliquot it and store at -20C (stored in PBS or water?).

    So I'm a bit confused by the datasheet and hopefully you can provide me some additional information.

    Read More

    Abcam community

    Verified customer

    Asked on Dec 03 2012

    Answer

    Thank you for contacting us.


    I have been in contact with the lab, I can confirm that ab9918 has already been reconstituted in 200ul sterile water. Since this antibody was lyophilised in PBS, this means that upon the addition of the water, the antibody has been re-dissolved in PBS. There is an error on the datasheet concerning the reconstitution in sterile water and we apologize for this and thank you for bringing this to our attention as it will allow us to update our datasheet.

    With regards to storage, we have decided to change the storage conditions to -20ºC instead of 4ºC simply so that the antibody’s activity will be stable for longer. As a rough guide, we suggest that an antibody’s shelf life at 4ºC is 1 year and at -20ºC is 5 years. I can reassure you though that there has been no change in the production of this antibody and that it should function exactly the same as before.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Free Rabbit monoclonal antibody with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

    Read More

    Abcam Scientific Support

    Answered on Dec 03 2012

    Question

    One doubt: some of this antibody only react with mouse, and you suggested human tissue. Is it correct?
    Thanks,

    Read More

    Abcam community

    Verified customer

    Asked on Aug 14 2012

    Answer

    Thank you for your email.

    There may be some antibodies where I might have suggested the human tissues or cells. I apologize for this. I have included the positive control information irrespective of species so similar mouse tissues sections can used as positive control.

    Actually the positive control information for each antibody is present on the datasheet; please check the datasheet. Alternatively, we always follow human protein Atlas database (http://www.proteinatlas.org/index.php). The second option is, searching positive control for other antibodies in catalogue against same target; the cell line or tissue sections tested with those antibodies could also be used as positive control. The third place to search for is literature.

    I hope this information will be helpful. Should you have any question please do not hesitate to contact me.

    Read More

    Abcam Scientific Support

    Answered on Aug 15 2012

    Question

    Our customer would like to know which are the best positive control
    and negative control for the antibodies below.
    1. anti Natural killer - ab36392
    2. anti- interferon gama - ab9918
    3. anti TNF alfa - ab34674
    4. anti TGF beta - ab15537
    5. anti neutrófilo - ab2557
    6. anti CD20 - ab9475
    7. anti células T CD8 - ab22378
    8. anti células T CD4 - ab51312
    She will do ICC and IHC-P assays.
    Thanks and best regards,

    Read More

    Abcam community

    Verified customer

    Asked on Aug 10 2012

    Answer

    Thank you for contacting us.

    The positive control for ab36392 is IL2 activatedcultured NK cells.

    ab9918; human tonsil sections, human cervical squamous cell carcinoma section

    ab34674; human artery or colon tissue sections

    ab15537; HepG2 cells. mouse brain sections, human placenta sections; Human hepatocellular liver carcinoma cell line; Human hepatocarcinoma tissue sections

    ab2557; mouse spleen tissue sections

    ab9475; human tonsil tissue sections

    ab22378; mouse spleen tissue sections

    ab51312; Thymus tissue sections, human pancreas tissue sections.

    The best database to search for positive control is Human Protein Atlas. Please click the link http://www.proteinatlas.org/index.php

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Use our products? Submit an Abreview. Earn rewards!
    https://www.abcam.com/abreviews

    Read More

    Abcam Scientific Support

    Answered on Aug 10 2012

    Question

    Hello



    The customer regarding protocol provide us the datasheet of ab2557, where it is write that the antibody must be stored at 4oC, not at -20oC. It is attached below.



    In addition, the customer said that, despite she had used mouse tissue, she did not understand the multiple unespecific dots at both techniques IHC and ICC.

    Read More

    Abcam community

    Verified customer

    Asked on May 18 2012

    Answer

    Thank you for your reply.

    It does look like we changed the storage instructions of ab2557. Since this product was tested in a guaranteed species and technique and she wasn't seeing any staining, we could offer a free of charge replacement order from the original order. Please let me know if that is what the customer would prefer.

    For the spotty staining in IHC, it may be that the antibody is binding to the blocking buffer. She should filter the blocking buffer before adding to the slide. Also she should run a no primary control to determine if the secondary is binding to something non-specifically in the slide. Also the tissue maynot washed enough orfixative may still present. And though they used a gentle permeabilization agent, pemeabilization may have damaged the membrane and removed the membrane protein
    Remove permeabilizing agent from your buffers.

    I hope this information helps. Please let me know what the customer would like to do with the ab2557.

    Read More

    Abcam Scientific Support

    Answered on May 18 2012

    Question


    A customer had some problems with some antibodies that she had bought.
    I attached below the questionnarie.
    Any doubts, please contact me again,
    Best regards,

    Read More

    Abcam community

    Verified customer

    Asked on May 15 2012

    Answer

    Thank you forhaving your customercomplete our questionnaire. I am sorry to hear that these antibodies are not providing satisfactory results for them. The details provided will enable us to investigate this case and will provide us with vital information for monitoring product quality.

    There are some issues with the antibodies that may be resulting in the lack of staining. Firstly, the neutrophil antibody ab2557 was stored in the refrigerator. The datasheet for this antibody says to store at -20C. The last order I have from you for this product is in January. It is possible that storing this antibody improperly for 4 months has affected its quality.

    Secondly, the NK antibody ab36392 is not tested in mouse, only rat. We do not guarantee this antibody to work in mouse. Does the customer have access to rat tissue to run a positive control? Also this is the antibody that was giving spotty staining. Have they run a no-primary control? Or tried filtering the blocking buffer?

    Lastly, the interferon gamma antibody ab9918 is not tested in IHC-P or ICC and is therefore not guaranteed to work in these applications.

    Since two of the antibodies are being used in untested ways and the neutrophil antibody was improperly stored, there are many reasons why the customer may not be seeing staining.

    I hope this information is helpful, and I thank you for your cooperation.

    Read More

    Abcam Scientific Support

    Answered on May 15 2012

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Interferon gamma antibody

    Inconclusive
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (thumus)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: EDTA(ventana CC1)
    Permeabilization
    No
    Specification
    thumus
    Fixative
    10% neutral formalin
    Read More

    Abcam user community

    Verified customer

    Submitted May 04 2017

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