Key features and details
- Rabbit monoclonal [EP1109Y] to Interferon gamma
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Interferon gamma antibody [EP1109Y]
See all Interferon gamma primary antibodies
DescriptionRabbit monoclonal [EP1109Y] to Interferon gamma
SpecificityThis antibody reacts with Interferon gamma.
Tested applicationsSuitable for: WBmore details
Unsuitable for: ICC/IF or IHC-P
Species reactivityReacts with: Human
Synthetic peptide within Human Interferon gamma aa 100-200. The exact sequence is proprietary.
- Human Interferon gamma recombinant protein
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.15% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab52746 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/2000. Detects a band of approximately 17 kDa (predicted molecular weight: 19 kDa).|
FunctionProduced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons.
Tissue specificityReleased primarily from activated T lymphocytes.
Involvement in diseaseIn Caucasians, genetic variation in IFNG is associated with the risk of aplastic anemia (AA) [MIM:609135]. AA is a rare disease in which the reduction of the circulating blood cells results from damage to the stem cell pool in bone marrow. In most patients, the stem cell lesion is caused by an autoimmune attack. T-lymphocytes, activated by an endogenous or exogenous, and most often unknown antigenic stimulus, secrete cytokines, including IFN-gamma, which would in turn be able to suppress hematopoiesis.
Sequence similaritiesBelongs to the type II (or gamma) interferon family.
modificationsProteolytic processing produces C-terminal heterogeneity, with proteins ending alternatively at Gly-150, Met-157 or Gly-161.
- Information by UniProt
- IF 1 antibody
- IFG antibody
- IFI antibody
Anti-Interferon gamma antibody [EP1109Y] (ab52746) at 1/1000 dilution + Human Interferon gamma recombinant protein at 0.015 µg
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 19 kDa
Blocking buffer: 5% NFDM/TBST
Diluting buffer: 5% NFDM/TBST
Anti-Interferon gamma antibody [EP1109Y] (ab52746) at 1/2000 dilution + recombinant protein at 0.01 µg
Goat anti-rabbit HRP labeled at 1/2000 dilution
Predicted band size: 19 kDa
Observed band size: 17 kDa why is the actual band size different from the predicted?
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab52746 has been referenced in 1 publication.
- Lian X et al. Vibrio parahaemolyticus elevates interferon alpha production in intestinal-like epithelial Caco-2 cells. Can J Microbiol 53:1084-90 (2007). PubMed: 18026229