Recombinant
RabMAb

Recombinant Anti-Interferon gamma antibody [EPR1108] (ab133566)

Reviews (3)Q&A (2)References (15)

Overview

  • Product name

    Anti-Interferon gamma antibody [EPR1108]
    See all Interferon gamma primary antibodies

  • Description

    Rabbit monoclonal [EPR1108] to Interferon gamma

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt or ICC/IF

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human Interferon gamma aa 1-100 (internal sequence). The exact sequence is proprietary.
    Database link: P01579

  • Positive control

    • WB: Recombinant Interferon gamma protein; MOLT4 and Jurkat cell lysates; Human ymph node and kidney lysates. Flow cyt: Jurkat cells. ICC/IF: Jurkat cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

Our Abpromise guarantee covers the use of ab133566 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 19 kDa.
  • Application notes
    Is unsuitable for Flow Cyt or ICC/IF.

    • Target

    • Function

      Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons.

    • Tissue specificity

      Released primarily from activated T lymphocytes.

    • Involvement in disease

      In Caucasians, genetic variation in IFNG is associated with the risk of aplastic anemia (AA) [MIM:609135]. AA is a rare disease in which the reduction of the circulating blood cells results from damage to the stem cell pool in bone marrow. In most patients, the stem cell lesion is caused by an autoimmune attack. T-lymphocytes, activated by an endogenous or exogenous, and most often unknown antigenic stimulus, secrete cytokines, including IFN-gamma, which would in turn be able to suppress hematopoiesis.

    • Sequence similarities

      Belongs to the type II (or gamma) interferon family.

    • Post-translational
      modifications

      Proteolytic processing produces C-terminal heterogeneity, with proteins ending alternatively at Gly-150, Met-157 or Gly-161.

    • Cellular localization

      Secreted.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • IF 1 antibody
      • IFG antibody
      • IFI antibody
      • IFN gamma antibody
      • IFN immune antibody
      • IFN, immune antibody
      • IFN-gamma antibody
      • IFNG antibody
      • IFNG_HUMAN antibody
      • Immune interferon antibody
      • Interferon gamma antibody
      • Type II Interferon antibody
      see all

    Images

    • Western blot - Anti-Interferon gamma antibody [EPR1108] (ab133566)
      Western blot - Anti-Interferon gamma antibody [EPR1108] (ab133566)
      All lanes : Anti-Interferon gamma antibody [EPR1108] (ab133566) at 1/1000 dilution (unpurified)

      Lane 1 : MOLT4 cell lysate
      Lane 2 : Jurkat cell lysate
      Lane 3 : Lymph node lysate
      Lane 4 : Human kidney lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 19 kDa

    • Western blot - Anti-Interferon gamma antibody [EPR1108] (ab133566)
      Western blot - Anti-Interferon gamma antibody [EPR1108] (ab133566)
      Anti-Interferon gamma antibody [EPR1108] (ab133566) at 1/2500 dilution (purified) + Rat brain lysate at 10 µg

      Secondary
      Rabbit monoclonal [EPR1108] to Interferon gamma (ab133566) at 1/10000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

      Predicted band size: 19 kDa



      Blocking buffer and concentration: 5% NFDM/TBST.

      Diluting buffer and concentration: 5% NFDM /TBST.

    • Western blot - Anti-Interferon gamma antibody [EPR1108] (ab133566)
      Western blot - Anti-Interferon gamma antibody [EPR1108] (ab133566)
      Anti-Interferon gamma antibody [EPR1108] (ab133566) at 1/5000 dilution (purified) + Mouse brain lysate at 20 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

      Predicted band size: 19 kDa



      Blocking buffer and concentration: 5% NFDM/TBST.

      Diluting buffer and concentration: 5% NFDM /TBST.

    • Western blot - Anti-Interferon gamma antibody [EPR1108] (ab133566)
      Western blot - Anti-Interferon gamma antibody [EPR1108] (ab133566)
      Anti-Interferon gamma antibody [EPR1108] (ab133566) at 1/2500 dilution (purified) + Molt-4 at 10 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

      Predicted band size: 19 kDa



      Blocking buffer and concentration: 5% NFDM/TBST.

      Diluting buffer and concentration: 5% NFDM /TBST.

    • Western blot - Anti-Interferon gamma antibody [EPR1108] (ab133566)
      Western blot - Anti-Interferon gamma antibody [EPR1108] (ab133566)
      Anti-Interferon gamma antibody [EPR1108] (ab133566) at 1/1000 dilution (unpurified) + Recombinant Interferon gamma protein at 0.005 µg

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 19 kDa



      Note: Molecular weight of recombinant protein is 17 kDa.

    References

    This product has been referenced in:

    • Qiu X  et al. Tumor-derived nanovesicles promote lung distribution of the therapeutic nanovector through repression of Kupffer cell-mediated phagocytosis. Theranostics 9:2618-2636 (2019). Read more (PubMed: 31131057) »
    • Jiang MC  et al. Production of Human IFN? Protein in Nicotiana benthamiana Plant through an Enhanced Expression System Based on Bamboo mosaic Virus. Viruses 11:N/A (2019). Read more (PubMed: 31163694) »
    See all 15 Publications for this product

    Publishing research using ab133566? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    1-5 of 5 Abreviews or Q&A

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Interferon gamma antibody [EPR1108]

     Good
    Abreviews
    This product is known to not work in this application or species.
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (Myocardium)
    Permeabilization
    No
    Specification
    Myocardium
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.5% · Temperature: 23°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Jun 22 2016

    Western blot abreview for Anti-Interferon gamma antibody [EPR1108]

     Good
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    25 µg
    Gel Running Conditions
    Reduced Denaturing
    Sample
    Mouse Cell lysate - whole cell (Hep G2)
    Specification
    Hep G2
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
    Read More

    Abcam user community

    Verified customer

    Submitted Dec 24 2014

    Western blot abreview for Anti-Interferon gamma antibody [EPR1108]

     Good
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (Serum)
    Gel Running Conditions
    Reduced Non-Denaturing (Native) (12)
    Loading amount
    40 µg
    Specification
    Serum
    Blocking step
    Odyssey Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 26°C
    Read More

    Abcam user community

    Verified customer

    Submitted Aug 25 2014

    Question

    Thanks you for your speedy reply. Can you please send me a copy of the ab133566 datasheet and also let me know if it is possible for me to obtain the ab82447 hybridoma so that I have the option to continue using this reagent?

    Thanks

    Read More
    Answer

    Thank you for your answer.

    I attached the datasheet for the Anti-Interferon gamma antibody [EPR1108] (ab133566) to this email. I hope this antibody might suit the experiment as an alternative.

    I can confirm, that it is not possible for us to provide you with the hybridoma for the ab82447. I sorry that i can not be of more help in this case and apologise for the inconvenience.

    Please let me know if you have further questions regarding the ab133566 or any other of our products. I hope this might be of help to you nevertheless and wish you all the best for your research.

    Read More

    Question

    Good Afternoon,
    I have been informed that the antibody product “Ab82247”, is no longer available and he has suggested that I contact technical support to discuss this point further and to identify an alternative. We have developed an assay at Novimmune using Ab82247 as a critical reagent to support our clinical trials and hence I really need to find a quick solution to this problem. Can we please arrange to speak on the telephone at your earliest convenience?

    Best wishes,

    Read More
    Answer

    Thank you for contacting us.

    I can confirm, that regrettably we cannot longer provide the ab82447. I apologise for this inconvenience. I would be pleased to help finding a suitable replacement for you.

    Could you please confirm what kind of conditions have to be fulfilled by a suitable antibody? What is the performed experiment and what samples will be used?

    We were looking at the Anti-Interferon gamma antibody [EPR1108] (ab133566). I would suggest to check the datasheet to see if this product might suit the performed experiment.


    I hope this information is helpful to you. Thank you for your cooperation. Please do not hesitate to contact us if you need any more advice or information.

    Read More

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