Name: Anti-Interferon gamma antibody [EPR1108]
Brand: RabMAb
SKU: ab133566
Rating: 4 (3 reviews)

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Western blot - Anti-Interferon gamma antibody [EPR1108] (ab133566)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR1108] to Interferon gamma
Suitable for: WB
Reacts with: Mouse, Rat, Human, Recombinant fragment

Product name

Anti-Interferon gamma antibody [EPR1108]
See all Interferon gamma primary antibodies
Description

Rabbit monoclonal [EPR1108] to Interferon gamma
Host species

Rabbit

Tested Applications & Species

Application	Species
WB	Mouse Rat Human Recombinant fragment

See all applications and species data

Immunogen

Synthetic peptide within Human Interferon gamma aa 1-100 (internal sequence). The exact sequence is proprietary.
Database link: P01579
Positive control
- WB: Recombinant Interferon gamma protein; MOLT4 and Jurkat cell lysates; Human ymph node and kidney lysates. Flow cyt: Jurkat cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 0.05% BSA, 59% PBS
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR1108
Isotype

IgG
Research areas

Alternative Versions
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
Positive Controls
- Jurkat whole cell lysate (ab7899)
- MOLT-4 whole cell lysate (ab7912)
Recombinant Protein
- Recombinant Human Interferon gamma protein (Active) (ab259377)

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab133566 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application	Species
Flow Cyt	Human
WB	Mouse Rat Human Recombinant fragment

Application	Abreviews	Notes
WB	(2)	1/1000 - 1/10000. Predicted molecular weight: 19 kDa.

Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 19 kDa.

Application notes

Is unsuitable for Flow Cyt or ICC/IF.

Function

Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons.
Tissue specificity

Released primarily from activated T lymphocytes.
Involvement in disease

In Caucasians, genetic variation in IFNG is associated with the risk of aplastic anemia (AA) [MIM:609135]. AA is a rare disease in which the reduction of the circulating blood cells results from damage to the stem cell pool in bone marrow. In most patients, the stem cell lesion is caused by an autoimmune attack. T-lymphocytes, activated by an endogenous or exogenous, and most often unknown antigenic stimulus, secrete cytokines, including IFN-gamma, which would in turn be able to suppress hematopoiesis.
Sequence similarities

Belongs to the type II (or gamma) interferon family.
Post-translational
modifications

Proteolytic processing produces C-terminal heterogeneity, with proteins ending alternatively at Gly-150, Met-157 or Gly-161.
Cellular localization

Secreted.
Target information above from: UniProt accession P01579 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 3458 Human
- Entrez Gene: 15978 Mouse
- Entrez Gene: 25712 Rat
- Omim: 147570 Human
- SwissProt: P01579 Human
- SwissProt: P01580 Mouse
- SwissProt: P01581 Rat
- Unigene: 856 Human
- Unigene: 240327 Mouse
- Unigene: 10795 Rat
see all
Alternative names
- IF 1 antibody
- IFG antibody
- IFI antibody
- IFN gamma antibody
- IFN immune antibody
- IFN, immune antibody
- IFN-gamma antibody
- IFNG antibody
- IFNG_HUMAN antibody
- Immune interferon antibody
- Interferon gamma antibody
- Type II Interferon antibody
see all

Western blot - Anti-Interferon gamma antibody [EPR1108] (ab133566)

All lanes : Anti-Interferon gamma antibody [EPR1108] (ab133566) at 1/1000 dilution (unpurified)

Lane 1 : MOLT4 cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : Lymph node lysate
Lane 4 : Human kidney lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 19 kDa
Western blot - Anti-Interferon gamma antibody [EPR1108] (ab133566)

Anti-Interferon gamma antibody [EPR1108] (ab133566) at 1/2500 dilution (purified) + Rat brain lysate at 10 µg

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution

Predicted band size: 19 kDa

Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
Western blot - Anti-Interferon gamma antibody [EPR1108] (ab133566)

Anti-Interferon gamma antibody [EPR1108] (ab133566) at 1/5000 dilution (purified) + Mouse brain lysate at 20 µg

Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

Predicted band size: 19 kDa

Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
Western blot - Anti-Interferon gamma antibody [EPR1108] (ab133566)

Anti-Interferon gamma antibody [EPR1108] (ab133566) at 1/2500 dilution (purified) + Molt-4 at 10 µg

Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

Predicted band size: 19 kDa

Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
Western blot - Anti-Interferon gamma antibody [EPR1108] (ab133566)

Anti-Interferon gamma antibody [EPR1108] (ab133566) at 1/1000 dilution (unpurified) + Recombinant Interferon gamma protein at 0.005 µg

Secondary
HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 19 kDa

Note: Molecular weight of recombinant protein is 17 kDa.
Anti-Interferon gamma antibody [EPR1108] (ab133566)

Flow cytometry protocols
Immunocytochemistry & immunofluorescence protocols
Western blot protocols

Click here to view the general protocols

Publishing research using ab133566? Please let us know so that we can cite the reference in this datasheet.

ab133566 has been referenced in 20 publications.

Gangisetty O et al. Transgenerational inheritance of fetal alcohol exposure adverse effects on immune gene interferon-?. Clin Epigenetics 12:70 (2020). PubMed: 32448218
Qiu X et al. Tumor-derived nanovesicles promote lung distribution of the therapeutic nanovector through repression of Kupffer cell-mediated phagocytosis. Theranostics 9:2618-2636 (2019). PubMed: 31131057
Jiang MC et al. Production of Human IFN? Protein in Nicotiana benthamiana Plant through an Enhanced Expression System Based on Bamboo mosaic Virus. Viruses 11:N/A (2019). PubMed: 31163694
Tang H et al. Inhibition of COX-2 and EGFR by Melafolone Improves Anti-PD-1 Therapy through Vascular Normalization and PD-L1 Downregulation in Lung Cancer. J Pharmacol Exp Ther 368:401-413 (2019). PubMed: 30591531
Armstrong H et al. Nigericin Promotes NLRP3-Independent Bacterial Killing in Macrophages. Front Immunol 10:2296 (2019). PubMed: 31632394

View all Publications for this product

Customer reviews and Q&As

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1-5 of 5 Abreviews or Q&A

Application

Immunocytochemistry/ Immunofluorescence

Sample

Mouse Cell (Myocardium)

Permeabilization

Specification

Myocardium

Blocking step

Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.5% · Temperature: 23°C

Fixative

Paraformaldehyde

Abcam user community

Verified customer

Submitted Jun 22 2016

Application

Western blot

Loading amount

25 µg

Gel Running Conditions

Reduced Denaturing

Sample

Mouse Cell lysate - whole cell (Hep G2)

Specification

Hep G2

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C

Abcam user community

Verified customer

Submitted Dec 24 2014

Application

Western blot

Sample

Mouse Tissue lysate - whole (Serum)

Gel Running Conditions

Reduced Non-Denaturing (Native) (12)

Loading amount

40 µg

Specification

Serum

Blocking step

Odyssey Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 26°C

Abcam user community

Verified customer

Submitted Aug 25 2014

Question

Thanks you for your speedy reply. Can you please send me a copy of the ab133566 datasheet and also let me know if it is possible for me to obtain the ab82447 hybridoma so that I have the option to continue using this reagent?

Thanks

Abcam community

Verified customer

Asked on Dec 18 2012

Answer

Thank you for your answer.

I attached the datasheet for the Anti-Interferon gamma antibody [EPR1108] (ab133566) to this email. I hope this antibody might suit the experiment as an alternative.

I can confirm, that it is not possible for us to provide you with the hybridoma for the ab82447. I sorry that i can not be of more help in this case and apologise for the inconvenience.

Please let me know if you have further questions regarding the ab133566 or any other of our products. I hope this might be of help to you nevertheless and wish you all the best for your research.

Abcam Scientific Support

Answered on Dec 18 2012

Question

Good Afternoon,
I have been informed that the antibody product “Ab82247”, is no longer available and he has suggested that I contact technical support to discuss this point further and to identify an alternative. We have developed an assay at Novimmune using Ab82247 as a critical reagent to support our clinical trials and hence I really need to find a quick solution to this problem. Can we please arrange to speak on the telephone at your earliest convenience?

Best wishes,

Abcam community

Verified customer

Asked on Dec 17 2012

Answer

Thank you for contacting us.

I can confirm, that regrettably we cannot longer provide the ab82447. I apologise for this inconvenience. I would be pleased to help finding a suitable replacement for you.

Could you please confirm what kind of conditions have to be fulfilled by a suitable antibody? What is the performed experiment and what samples will be used?

We were looking at the Anti-Interferon gamma antibody [EPR1108] (ab133566). I would suggest to check the datasheet to see if this product might suit the performed experiment.

I hope this information is helpful to you. Thank you for your cooperation. Please do not hesitate to contact us if you need any more advice or information.

Abcam Scientific Support

Answered on Dec 17 2012

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Key features and details

Overview

Product name

Description

Host species

Tested Applications & Species

Immunogen

Positive control

General notes

Properties

Form

Storage instructions

Storage buffer

Purity

Clonality

Clone number

Isotype

Research areas

Associated products

Alternative Versions

Isotype control

Positive Controls

Recombinant Protein

Applications

The Abpromise guarantee

Target

Function

Tissue specificity

Involvement in disease

Sequence similarities

Post-translationalmodifications

Cellular localization

Database links

Alternative names

Images

Protocols

Datasheets and documents

References (20)

Customer reviews and Q&As

Immunocytochemistry/ Immunofluorescence abreview for Anti-Interferon gamma antibody [EPR1108]

Western blot abreview for Anti-Interferon gamma antibody [EPR1108]

Western blot abreview for Anti-Interferon gamma antibody [EPR1108]

Post-translational
modifications