Recombinant Anti-Interferon gamma antibody [EPR1108] (ab133566)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR1108] to Interferon gamma
- Suitable for: WB
- Reacts with: Mouse, Rat, Human, Recombinant fragment
Overview
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Product name
Anti-Interferon gamma antibody [EPR1108]
See all Interferon gamma primary antibodies -
Description
Rabbit monoclonal [EPR1108] to Interferon gamma -
Host species
Rabbit -
Tested Applications & Species
Application Species WB MouseRatHumanRecombinant fragment -
Immunogen
Synthetic peptide within Human Interferon gamma aa 1-100 (internal sequence). The exact sequence is proprietary.
Database link: P01579 -
Positive control
- WB: Recombinant Interferon gamma protein; MOLT4 and Jurkat cell lysates; Human ymph node and kidney lysates. Flow cyt: Jurkat cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 0.05% BSA, 59% PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR1108 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab133566 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
Application | Species |
---|---|
Flow Cyt |
Human
|
WB |
Mouse
Rat
Human
Recombinant fragment
|
Application | Abreviews | Notes |
---|---|---|
WB | (2) |
1/1000 - 1/10000. Predicted molecular weight: 19 kDa.
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Notes |
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WB
1/1000 - 1/10000. Predicted molecular weight: 19 kDa. |
Target
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Function
Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons. -
Tissue specificity
Released primarily from activated T lymphocytes. -
Involvement in disease
In Caucasians, genetic variation in IFNG is associated with the risk of aplastic anemia (AA) [MIM:609135]. AA is a rare disease in which the reduction of the circulating blood cells results from damage to the stem cell pool in bone marrow. In most patients, the stem cell lesion is caused by an autoimmune attack. T-lymphocytes, activated by an endogenous or exogenous, and most often unknown antigenic stimulus, secrete cytokines, including IFN-gamma, which would in turn be able to suppress hematopoiesis. -
Sequence similarities
Belongs to the type II (or gamma) interferon family. -
Post-translational
modificationsProteolytic processing produces C-terminal heterogeneity, with proteins ending alternatively at Gly-150, Met-157 or Gly-161. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 3458 Human
- Entrez Gene: 15978 Mouse
- Entrez Gene: 25712 Rat
- Omim: 147570 Human
- SwissProt: P01579 Human
- SwissProt: P01580 Mouse
- SwissProt: P01581 Rat
- Unigene: 856 Human
see all -
Alternative names
- IF 1 antibody
- IFG antibody
- IFI antibody
see all
Images
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All lanes : Anti-Interferon gamma antibody [EPR1108] (ab133566) at 1/1000 dilution (unpurified)
Lane 1 : MOLT4 cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : Lymph node lysate
Lane 4 : Human kidney lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 19 kDa -
Anti-Interferon gamma antibody [EPR1108] (ab133566) at 1/2500 dilution (purified) + Rat brain lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution
Predicted band size: 19 kDaBlocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
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Anti-Interferon gamma antibody [EPR1108] (ab133566) at 1/5000 dilution (purified) + Mouse brain lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Predicted band size: 19 kDaBlocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
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Anti-Interferon gamma antibody [EPR1108] (ab133566) at 1/2500 dilution (purified) + Molt-4 at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Predicted band size: 19 kDaBlocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
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Anti-Interferon gamma antibody [EPR1108] (ab133566) at 1/1000 dilution (unpurified) + Recombinant Interferon gamma protein at 0.005 µg
Secondary
HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 19 kDa
Note: Molecular weight of recombinant protein is 17 kDa.
Protocols
References (20)
ab133566 has been referenced in 20 publications.
- Gangisetty O et al. Transgenerational inheritance of fetal alcohol exposure adverse effects on immune gene interferon-?. Clin Epigenetics 12:70 (2020). PubMed: 32448218
- Qiu X et al. Tumor-derived nanovesicles promote lung distribution of the therapeutic nanovector through repression of Kupffer cell-mediated phagocytosis. Theranostics 9:2618-2636 (2019). PubMed: 31131057
- Jiang MC et al. Production of Human IFN? Protein in Nicotiana benthamiana Plant through an Enhanced Expression System Based on Bamboo mosaic Virus. Viruses 11:N/A (2019). PubMed: 31163694
- Tang H et al. Inhibition of COX-2 and EGFR by Melafolone Improves Anti-PD-1 Therapy through Vascular Normalization and PD-L1 Downregulation in Lung Cancer. J Pharmacol Exp Ther 368:401-413 (2019). PubMed: 30591531
- Armstrong H et al. Nigericin Promotes NLRP3-Independent Bacterial Killing in Macrophages. Front Immunol 10:2296 (2019). PubMed: 31632394