Recombinant Anti-Interferon gamma antibody [EPR1108] (ab133566)
![Western blot - Anti-Interferon gamma antibody [EPR1108] (ab133566)](https://www.abcam.com/ps/products/133/ab133566/Images/ab133566-147333-anti-interferon-gamma-antibody-epr1108-western-blot.jpg "Western blot - Anti-Interferon gamma antibody [EPR1108] (ab133566)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR1108] to Interferon gamma
- Suitable for: WB
- Reacts with: Human, Recombinant fragment
Related conjugates and formulations
Overview
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Product name
Anti-Interferon gamma antibody [EPR1108]
See all Interferon gamma primary antibodies -
Description
Rabbit monoclonal [EPR1108] to Interferon gamma -
Host species
Rabbit -
Specificity
Immunogen used to generate ab133566 does not show any identities with mouse/rat Interferon gamma. Not expected to react with mouse or rat.
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Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt or ICC/IF -
Species reactivity
Reacts with: Human, Recombinant fragment -
Immunogen
Synthetic peptide within Human Interferon gamma aa 1-100 (internal sequence). The exact sequence is proprietary.
Database link: P01579 -
Positive control
- WB: Recombinant Interferon gamma protein; MOLT4 and Jurkat cell lysates; Human lymph node and kidney lysates. Flow Cyt: Jurkat cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 0.05% BSA, 59% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR1108 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab133566 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (2) |
1/1000 - 1/10000. Predicted molecular weight: 19 kDa.
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| Notes |
|---|
|
WB
1/1000 - 1/10000. Predicted molecular weight: 19 kDa. |
Application notes
Is unsuitable for Flow Cyt or ICC/IF.
Target
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Function
Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons. -
Tissue specificity
Released primarily from activated T lymphocytes. -
Involvement in disease
In Caucasians, genetic variation in IFNG is associated with the risk of aplastic anemia (AA) [MIM:609135]. AA is a rare disease in which the reduction of the circulating blood cells results from damage to the stem cell pool in bone marrow. In most patients, the stem cell lesion is caused by an autoimmune attack. T-lymphocytes, activated by an endogenous or exogenous, and most often unknown antigenic stimulus, secrete cytokines, including IFN-gamma, which would in turn be able to suppress hematopoiesis. -
Sequence similarities
Belongs to the type II (or gamma) interferon family. -
Post-translational
modificationsProteolytic processing produces C-terminal heterogeneity, with proteins ending alternatively at Gly-150, Met-157 or Gly-161. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 3458 Human
- Omim: 147570 Human
- SwissProt: P01579 Human
- Unigene: 856 Human
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Alternative names
- IF 1 antibody
- IFG antibody
- IFI antibody
see all
Images
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Western blot - Anti-Interferon gamma antibody [EPR1108] (ab133566)All lanes : Anti-Interferon gamma antibody [EPR1108] (ab133566) at 1/1000 dilution (unpurified)
Lane 1 : MOLT4 cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : Lymph node lysate
Lane 4 : Human kidney lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 19 kDa -
![Western blot - Anti-Interferon gamma antibody [EPR1108] (ab133566)](https://www.abcam.com/ps/products/133/ab133566/Images/ab133566-251266-anti-interferon-gamma-antibody-epr1108-western-blot.jpg)
Western blot - Anti-Interferon gamma antibody [EPR1108] (ab133566)Anti-Interferon gamma antibody [EPR1108] (ab133566) at 1/2500 dilution (purified) + Molt-4 at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Predicted band size: 19 kDaBlocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
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![Western blot - Anti-Interferon gamma antibody [EPR1108] (ab133566)](https://www.abcam.com/ps/products/133/ab133566/Images/ab133566-147334-anti-interferon-gamma-antibody-epr1108-western-blot.jpg)
Western blot - Anti-Interferon gamma antibody [EPR1108] (ab133566)Anti-Interferon gamma antibody [EPR1108] (ab133566) at 1/1000 dilution (unpurified) + Recombinant Interferon gamma protein at 0.005 µg
Secondary
HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 19 kDa
Note: Molecular weight of recombinant protein is 17 kDa. -
![Anti-Interferon gamma antibody [EPR1108] (ab133566)](https://www.abcam.com/ps/products/133/ab133566/Images/ab133566-8-benefits-of-recombinant-antibodies.png)
Anti-Interferon gamma antibody [EPR1108] (ab133566)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (23)
ab133566 has been referenced in 23 publications.
- Kanai R et al. Interferon-? enhances the therapeutic effect of mesenchymal stem cells on experimental renal fibrosis. Sci Rep 11:850 (2021). PubMed: 33441701
- Gangisetty O et al. Transgenerational inheritance of fetal alcohol exposure adverse effects on immune gene interferon-?. Clin Epigenetics 12:70 (2020). PubMed: 32448218
- Pereira BLB et al. Spondias mombin L. attenuates ventricular remodelling after myocardial infarction associated with oxidative stress and inflammatory modulation. J Cell Mol Med 24:7862-7872 (2020). PubMed: 32468694
- He Q et al. Neuroinflammation, Oxidative Stress, and Neurogenesis in a Mouse Model of Chronic Fatigue Syndrome, and the Treatment with Kampo Medicine. Biol Pharm Bull 43:110-115 (2020). PubMed: 31902915
- Qiu X et al. Tumor-derived nanovesicles promote lung distribution of the therapeutic nanovector through repression of Kupffer cell-mediated phagocytosis. Theranostics 9:2618-2636 (2019). PubMed: 31131057