Recombinant
RabMAb

Recombinant Anti-Interferon gamma antibody [EPR21704] - BSA and Azide free (ab231301)

Overview

  • Product name

    Anti-Interferon gamma antibody [EPR21704] - BSA and Azide free
    See all Interferon gamma primary antibodies
  • Description

    Rabbit monoclonal [EPR21704] to Interferon gamma - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, Flow Cytmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human Interferon gamma aa 1 to the C-terminus. The exact sequence is proprietary.
    Database link: P01579

  • Positive control

    • Flow Cyt: NK-92 cells treated with 80 µM ab120297 PMA (Phorbol-12-myristate-13-acetate) and 3 µM Ionomycin for 5 hours, then 300 ng/ml BFA for 4 hours. IHC-P: NK-92 cells treated with 80 µM ab120297 PMA (Phorbol-12-myristate-13-acetate) and 3 µM Ionomycin for 5 hours, then 300 ng/ml BFA for 4 hours and human tonsil tissue.
  • General notes

    Ab231301 is the carrier-free version of ab231036. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab231301 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab231301 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 19 kDa.
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Flow Cyt Use at an assay dependent concentration.

Target

  • Function

    Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons.
  • Tissue specificity

    Released primarily from activated T lymphocytes.
  • Involvement in disease

    In Caucasians, genetic variation in IFNG is associated with the risk of aplastic anemia (AA) [MIM:609135]. AA is a rare disease in which the reduction of the circulating blood cells results from damage to the stem cell pool in bone marrow. In most patients, the stem cell lesion is caused by an autoimmune attack. T-lymphocytes, activated by an endogenous or exogenous, and most often unknown antigenic stimulus, secrete cytokines, including IFN-gamma, which would in turn be able to suppress hematopoiesis.
  • Sequence similarities

    Belongs to the type II (or gamma) interferon family.
  • Post-translational
    modifications

    Proteolytic processing produces C-terminal heterogeneity, with proteins ending alternatively at Gly-150, Met-157 or Gly-161.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Database links

  • Alternative names

    • IF 1 antibody
    • IFG antibody
    • IFI antibody
    • IFN gamma antibody
    • IFN immune antibody
    • IFN, immune antibody
    • IFN-gamma antibody
    • IFNG antibody
    • IFNG_HUMAN antibody
    • Immune interferon antibody
    • Interferon gamma antibody
    • Type II Interferon antibody
    see all

Images

  • Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized NK-92 (human malignant non-Hodgkin's lymphoma natural killer cell) cell line treated with 80 μM ab120297 PMA (Phorbol-12-myristate-13-acetate) and 3 μM Ionomycin for 5 hours, then 300 ng/ml BFA was added for 4 hours labeling Interferon gamma with ab231036 at 1/60 (red) and untreated control (green). Compared with a Rabbit monoclonal IgG-Isotype Control (ab172730) (black) and an unlabeled control (cells incubated with secondary anibody only) (blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077), at 1/2000 dilution was used as the secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab231036).

  • Immunohistochemical analysis of paraffin-embedded NK-92 cells labeling Interferon gamma with ab231036 at 1/500 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP) (ab97051). Nearly no staining on untreated NK92 cells (A) and positive staining on treated NK92 cells (B) (treated with 80 μM ab120297 PMA (Phorbol-12-myristate-13-acetate) and 3 μM Ionomycin for 5 hours, then with 300 ng/ml BFA for 4 hours) is observed (PMID:23129404). Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP) (ab97051).

    Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab231036).

  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling Interferon gamma with ab231036 at 1/500 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP) (ab97051). Sporadic cytoplasmic staining in limphocytes of human tonsil is observed (PMID: 21838712). Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP) (ab97051).

    Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab231036).

References

ab231301 has not yet been referenced specifically in any publications.

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