Anti-Interferon gamma antibody [F12] (ab8096)


  • Product name
    Anti-Interferon gamma antibody [F12]
    See all Interferon gamma primary antibodies
  • Description
    Mouse monoclonal [F12] to Interferon gamma
  • Host species
  • Specificity
    Monoclonal antibody F14 binds and neutralizes both natural and recombinant human gamma Interferon. Cross-reactivity with other cytokines has not been found. In general, monoclonal antibodies to gamma Interferon are able to inhibit Shwartzman reactions (reviewed by A. Billiau, 1988) and in the murine system appear to protect NZB mice against spontaneous development of autoimmune disease (Jacob et el., 1987).
  • Tested applications
    Suitable for: ELISA, IHC-Fr, Neutralising, IPmore details
  • Species reactivity
    Reacts with: Human
  • General notes
    One neutralizing unit is defined as the amount of antibody sufficient for neutralizing one unit of human gamma Interferon as calibrated against NIH standard Gg23-901-530. For the determination of the neutralizing activity a cytopathic inhibition assay was used.



Our Abpromise guarantee covers the use of ab8096 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
Neutralising Use at an assay dependent concentration. Binds and neutralizes both natural and recombinant human gamma interferon.
IP Use at an assay dependent concentration.


  • Function
    Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons.
  • Tissue specificity
    Released primarily from activated T lymphocytes.
  • Involvement in disease
    In Caucasians, genetic variation in IFNG is associated with the risk of aplastic anemia (AA) [MIM:609135]. AA is a rare disease in which the reduction of the circulating blood cells results from damage to the stem cell pool in bone marrow. In most patients, the stem cell lesion is caused by an autoimmune attack. T-lymphocytes, activated by an endogenous or exogenous, and most often unknown antigenic stimulus, secrete cytokines, including IFN-gamma, which would in turn be able to suppress hematopoiesis.
  • Sequence similarities
    Belongs to the type II (or gamma) interferon family.
  • Post-translational
    Proteolytic processing produces C-terminal heterogeneity, with proteins ending alternatively at Gly-150, Met-157 or Gly-161.
  • Cellular localization
  • Information by UniProt
  • Database links
  • Alternative names
    • IFG antibody
    • IFI antibody
    • IFN gamma antibody
    • IFN, immune antibody
    • IFN-gamma antibody
    • IFNG antibody
    • IFNG_HUMAN antibody
    • Immune interferon antibody
    • Interferon gamma antibody
    see all


ab8096 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

This antibody has only been tested in frozen sections, therefore we can't guarantee results.

The two monoclonals can be used in Elisa as a sandwich pair. However both antibodies contain BSA. This makes the antibodies unuseful as coating antibody. We suggest that you look at our complete optimised Elisa test kits: ab7440, ab7441, ab7442

No, we do not stock these IFN-gamma antibodies


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