Anti-Interferon gamma antibody [GIF-3] (ab9801)

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Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab9801 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Indirect ELISA 1/1000 - 1/10000.
Sandwich ELISA Use at an assay dependent dilution. Can be paired for Sandwich ELISA with Mouse monoclonal [GIF-10] to Interferon gamma (ab9805). PubMed: 9458493Used as the detection antibody in conjuction with recommended pair. sELISA can be used to determine IFN-g concentration in the cultures of human peripheral blood mononuclear cells.
Inhibition Assay Use at an assay dependent dilution. Can be used to neutralize the antiviral activity of IFN-g: The bioassay was performed using the L41 cell line and mouse encephalomyocarditis virus (EMCV). Briefly, interferon was pre-incubated with the ab for 2 h at RT and then added to the cell monolayer. 24 h later cells were infected with EMCV and cultured for further 24 h. Antiviral activity of interferon was evaluated using crystal-violet staining. PAbs to respective interferons and MAb isotype control were used as controls.
WB 1/500 - 1/1000. Detects a band of approximately 16-21 kDa.

Target

  • Function

    Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons.

  • Tissue specificity

    Released primarily from activated T lymphocytes.

  • Involvement in disease

    In Caucasians, genetic variation in IFNG is associated with the risk of aplastic anemia (AA) [MIM:609135]. AA is a rare disease in which the reduction of the circulating blood cells results from damage to the stem cell pool in bone marrow. In most patients, the stem cell lesion is caused by an autoimmune attack. T-lymphocytes, activated by an endogenous or exogenous, and most often unknown antigenic stimulus, secrete cytokines, including IFN-gamma, which would in turn be able to suppress hematopoiesis.

  • Sequence similarities

    Belongs to the type II (or gamma) interferon family.

  • Post-translational
    modifications

    Proteolytic processing produces C-terminal heterogeneity, with proteins ending alternatively at Gly-150, Met-157 or Gly-161.

  • Cellular localization

    Secreted.
  • Information by UniProt

  • Database links

    • Alternative names

      • IF 1 antibody
      • IFG antibody
      • IFI antibody
      • IFN gamma antibody
      • IFN immune antibody
      • IFN, immune antibody
      • IFN-gamma antibody
      • IFNG antibody
      • IFNG_HUMAN antibody
      • Immune interferon antibody
      • Interferon gamma antibody
      • Type II Interferon antibody
      see all

    References

    This product has been referenced in:

    • Hou J  et al. PTPROt maintains T cell immunity in the microenvironment of hepatocellular carcinoma. J Mol Cell Biol 7:338-50 (2015). Read more (PubMed: 26117839) »
    • Huang H  et al. A specific docking site for DNA polymerase {alpha}-primase on the SV40 helicase is required for viral primosome activity, but helicase activity is dispensable. J Biol Chem 285:33475-84 (2010). WB . Read more (PubMed: 20685648) »
    See all 5 Publications for this product

    Publishing research using ab9801? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    1-3 of 3 Abreviews or Q&A

    Question

    Unpublished compared to last week ab9657, ab9658. Another possible pair: ab9801, ab9805 (monoclones with different epitopes)? Has this specifically been tested? Sensitivity? Problems in the past with Inteferon gamma detection.

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    Answer

    Yes, antibodies of clones GIF3 and GIF-10 have been used in a sandwich ELISA. The sensitivity of the assay is 300 pg/ml. For the sandwich ELISA, antibody GIF-3 was labelled with biotin. Specific reference for this is Osna N et al. Chronic hepatitis C: T-helper1/T-helper2 imbalance could cause virus persistence in peripheral blood. Scand J Clin Lab Invest 57:703-10 (1997). sELISA. PubMed: 9458493.

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    Question

    Customer has reported getting a very faint band with the lot# 200502. A Previous lt had worked well for her.

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    Answer

    Thank you very much for your patience and for sending me the images of your blots. I have looked into this issue and for your two orders for this antibody you received two different lots. As we have had no other complaints regarding this antibody, my hunch is that your vial went off during shipping. With both vials of ab9801, you used the same protocol and samples? We have a newer lot in stock and I will send you a free of charge replacement if you would like. Just let me know.

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    Question

    Dear Sir, We bought this antibody in September last year. May I ask your help to check the problems as below. (1) ab9801 Mouse anti-Human IFN-r ---------> the kd size to be identified of IFN-r Kind Regards,

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    Answer

    The MW of IFN-gamma is approximately 17 kDa.

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