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  1. Link

    interferon-gamma-antibody-gif-3-ab9801.pdf

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Immunology Innate Immunity Cytokines Interferons
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Anti-Interferon gamma antibody [GIF-3] (ab9801)

  • Datasheet
  • SDS
Submit a review Q&A (3)References (6)

Product price, shipping and contact information

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Promotion Information

Abpromise

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Find out more.

Key features and details

  • Mouse monoclonal [GIF-3] to Interferon gamma
  • Suitable for: Sandwich ELISA, Indirect ELISA, Inhibition Assay, WB
  • Reacts with: Human
  • Isotype: IgG1

Get better batch-to-batch reproducibility with a recombinant antibody

Product image
Anti-Interferon gamma antibody [EPR21704] (ab231036)
  • Research with confidence – consistent and reproducible results with every batch
  • Long-term and scalable supply – powered by recombinant technology for fast production
  • Success from the first experiment – confirmed specificity through extensive validation
  • Ethical standards compliant – production is animal-free

Overview

  • Product name

    Anti-Interferon gamma antibody [GIF-3]
    See all Interferon gamma primary antibodies
  • Description

    Mouse monoclonal [GIF-3] to Interferon gamma
  • Host species

    Mouse
  • Tested applications

    Suitable for: Sandwich ELISA, Indirect ELISA, Inhibition Assay, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant full length protein corresponding to Interferon gamma.

  • Positive control

    • Purchase matching WB positive control:Recombinant Human Interferon gamma protein
  • General notes

    This product was changed from ascites to tissue culture supernatant on 28/11/2017. Please note that the dilutions may need to be adjusted accordingly.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.1% Sodium azide
    Constituent: PBS

    No preservative, sterile filtered
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    GIF-3
  • Myeloma

    NS0/1
  • Isotype

    IgG1
  • Light chain type

    unknown
  • Research areas

    • Immunology
    • Innate Immunity
    • Cytokines
    • Interferons
    • Stem Cells
    • Signaling Pathways
    • TGF beta
    • Interferon gamma
    • Cancer
    • Tumor immunology
    • Cytokines
    • Interferons
    • Immunology
    • Adaptive Immunity
    • Regulatory T Cells
    • Neuroscience
    • Processes

Associated products

  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
  • Recombinant Protein

    • Recombinant Human Interferon gamma protein (Active) (ab259377)
  • sELISA pair antibody

    • Anti-Interferon gamma antibody [GIF-10] (ab9805)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab9801 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Mouse monoclonal [GIF-10] to Interferon gamma (ab9805). PubMed: 9458493

Used as the detection antibody in conjuction with recommended pair.  sELISA can be used to determine IFN-g concentration in the cultures of human peripheral blood mononuclear cells.

Indirect ELISA
Use at an assay dependent concentration.
Inhibition Assay
Use at an assay dependent concentration.

Can be used to neutralize the antiviral activity of IFN-g: The bioassay was performed using the L41 cell line and mouse encephalomyocarditis virus (EMCV). Briefly, interferon was pre-incubated with the ab for 2 h at RT and then added to the cell monolayer. 24 h later cells were infected with EMCV and cultured for further 24 h. Antiviral activity of interferon was evaluated using crystal-violet staining. PAbs to respective interferons and MAb isotype control were used as controls.

WB
Use at an assay dependent concentration.
Notes
Sandwich ELISA
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Mouse monoclonal [GIF-10] to Interferon gamma (ab9805). PubMed: 9458493

Used as the detection antibody in conjuction with recommended pair.  sELISA can be used to determine IFN-g concentration in the cultures of human peripheral blood mononuclear cells.

Indirect ELISA
Use at an assay dependent concentration.
Inhibition Assay
Use at an assay dependent concentration.

Can be used to neutralize the antiviral activity of IFN-g: The bioassay was performed using the L41 cell line and mouse encephalomyocarditis virus (EMCV). Briefly, interferon was pre-incubated with the ab for 2 h at RT and then added to the cell monolayer. 24 h later cells were infected with EMCV and cultured for further 24 h. Antiviral activity of interferon was evaluated using crystal-violet staining. PAbs to respective interferons and MAb isotype control were used as controls.

WB
Use at an assay dependent concentration.

Target

  • Function

    Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons.
  • Tissue specificity

    Released primarily from activated T lymphocytes.
  • Involvement in disease

    In Caucasians, genetic variation in IFNG is associated with the risk of aplastic anemia (AA) [MIM:609135]. AA is a rare disease in which the reduction of the circulating blood cells results from damage to the stem cell pool in bone marrow. In most patients, the stem cell lesion is caused by an autoimmune attack. T-lymphocytes, activated by an endogenous or exogenous, and most often unknown antigenic stimulus, secrete cytokines, including IFN-gamma, which would in turn be able to suppress hematopoiesis.
  • Sequence similarities

    Belongs to the type II (or gamma) interferon family.
  • Post-translational
    modifications

    Proteolytic processing produces C-terminal heterogeneity, with proteins ending alternatively at Gly-150, Met-157 or Gly-161.
  • Cellular localization

    Secreted.
  • Target information above from: UniProt accession P01579 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 3458 Human
    • Omim: 147570 Human
    • SwissProt: P01579 Human
    • Unigene: 856 Human
    • Alternative names

      • IF 1 antibody
      • IFG antibody
      • IFI antibody
      • ifn g antibody
      • IFN gamma antibody
      • IFN immune antibody
      • IFN, immune antibody
      • IFN-gamma antibody
      • IFNG antibody
      • IFNG_HUMAN antibody
      • Immune interferon antibody
      • Interferon gamma antibody
      • Type II Interferon antibody
      see all

    Protocols

    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (6)

    Publishing research using ab9801? Please let us know so that we can cite the reference in this datasheet.

    ab9801 has been referenced in 6 publications.

    • Liang Z & Tang F The potency of lncRNA MALAT1/miR-155/CTLA4 axis in altering Th1/Th2 balance of asthma. Biosci Rep 40:N/A (2020). PubMed: 31909418
    • Hou J  et al. PTPROt maintains T cell immunity in the microenvironment of hepatocellular carcinoma. J Mol Cell Biol 7:338-50 (2015). PubMed: 26117839
    • Huang H  et al. A specific docking site for DNA polymerase {alpha}-primase on the SV40 helicase is required for viral primosome activity, but helicase activity is dispensable. J Biol Chem 285:33475-84 (2010). WB . PubMed: 20685648
    • Reddy PK  et al. Increased yield of high purity recombinant human interferon-gamma utilizing reversed phase column chromatography. Protein Expr Purif 52:123-30 (2007). PubMed: 17049266
    • Poluektova L  et al. Immunoregulatory effects of N9-benzyl- and N7-benzyl-8-bromoguanines. Int J Immunopharmacol 21:777-92 (1999). PubMed: 10576622
    • Osna N  et al. Chronic hepatitis C: T-helper1/T-helper2 imbalance could cause virus persistence in peripheral blood. Scand J Clin Lab Invest 57:703-10 (1997). Sandwich ELISA . PubMed: 9458493

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-3 of 3 Abreviews or Q&A

    Question

    Unpublished compared to last week ab9657, ab9658. Another possible pair: ab9801, ab9805 (monoclones with different epitopes)? Has this specifically been tested? Sensitivity? Problems in the past with Inteferon gamma detection.

    Read More

    Abcam community

    Verified customer

    Asked on Dec 01 2010

    Answer

    Yes, antibodies of clones GIF3 and GIF-10 have been used in a sandwich ELISA. The sensitivity of the assay is 300 pg/ml. For the sandwich ELISA, antibody GIF-3 was labelled with biotin. Specific reference for this is Osna N et al. Chronic hepatitis C: T-helper1/T-helper2 imbalance could cause virus persistence in peripheral blood. Scand J Clin Lab Invest 57:703-10 (1997). sELISA. PubMed: 9458493.

    Read More

    Abcam Scientific Support

    Answered on Dec 01 2010

    Question

    Customer has reported getting a very faint band with the lot# 200502. A Previous lt had worked well for her.

    Read More

    Abcam community

    Verified customer

    Asked on May 25 2004

    Answer

    Thank you very much for your patience and for sending me the images of your blots. I have looked into this issue and for your two orders for this antibody you received two different lots. As we have had no other complaints regarding this antibody, my hunch is that your vial went off during shipping. With both vials of ab9801, you used the same protocol and samples? We have a newer lot in stock and I will send you a free of charge replacement if you would like. Just let me know.

    Read More

    Abcam Scientific Support

    Answered on Jun 10 2004

    Question

    Dear Sir, We bought this antibody in September last year. May I ask your help to check the problems as below. (1) ab9801 Mouse anti-Human IFN-r ---------> the kd size to be identified of IFN-r Kind Regards,

    Read More

    Abcam community

    Verified customer

    Asked on Feb 26 2003

    Answer

    The MW of IFN-gamma is approximately 17 kDa.

    Read More

    Abcam Scientific Support

    Answered on Mar 03 2003

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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