Anti-Interferon gamma antibody [GIF-3] (ab9801)
Key features and details
- Mouse monoclonal [GIF-3] to Interferon gamma
- Suitable for: Sandwich ELISA, Indirect ELISA, Inhibition Assay, WB
- Reacts with: Human
- Isotype: IgG1
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-Interferon gamma antibody [GIF-3]
See all Interferon gamma primary antibodies -
Description
Mouse monoclonal [GIF-3] to Interferon gamma -
Host species
Mouse -
Tested applications
Suitable for: Sandwich ELISA, Indirect ELISA, Inhibition Assay, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein corresponding to Interferon gamma.
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Positive control
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General notes
This product was changed from ascites to tissue culture supernatant on 28/11/2017. Please note that the dilutions may need to be adjusted accordingly.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.1% Sodium azide
Constituent: PBS
No preservative, sterile filtered -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
GIF-3 -
Myeloma
NS0/1 -
Isotype
IgG1 -
Light chain type
unknown -
Research areas
Associated products
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Isotype control
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Recombinant Protein
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sELISA pair antibody
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab9801 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Sandwich ELISA |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Mouse monoclonal [GIF-10] to Interferon gamma (ab9805). PubMed: 9458493
Used as the detection antibody in conjuction with recommended pair. sELISA can be used to determine IFN-g concentration in the cultures of human peripheral blood mononuclear cells. |
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| Indirect ELISA |
Use at an assay dependent concentration.
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| Inhibition Assay |
Use at an assay dependent concentration.
Can be used to neutralize the antiviral activity of IFN-g: The bioassay was performed using the L41 cell line and mouse encephalomyocarditis virus (EMCV). Briefly, interferon was pre-incubated with the ab for 2 h at RT and then added to the cell monolayer. 24 h later cells were infected with EMCV and cultured for further 24 h. Antiviral activity of interferon was evaluated using crystal-violet staining. PAbs to respective interferons and MAb isotype control were used as controls. |
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| WB |
Use at an assay dependent concentration.
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| Notes |
|---|
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Sandwich ELISA
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Mouse monoclonal [GIF-10] to Interferon gamma (ab9805). PubMed: 9458493 Used as the detection antibody in conjuction with recommended pair. sELISA can be used to determine IFN-g concentration in the cultures of human peripheral blood mononuclear cells. |
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Indirect ELISA
Use at an assay dependent concentration. |
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Inhibition Assay
Use at an assay dependent concentration. Can be used to neutralize the antiviral activity of IFN-g: The bioassay was performed using the L41 cell line and mouse encephalomyocarditis virus (EMCV). Briefly, interferon was pre-incubated with the ab for 2 h at RT and then added to the cell monolayer. 24 h later cells were infected with EMCV and cultured for further 24 h. Antiviral activity of interferon was evaluated using crystal-violet staining. PAbs to respective interferons and MAb isotype control were used as controls. |
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WB
Use at an assay dependent concentration. |
Target
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Function
Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons. -
Tissue specificity
Released primarily from activated T lymphocytes. -
Involvement in disease
In Caucasians, genetic variation in IFNG is associated with the risk of aplastic anemia (AA) [MIM:609135]. AA is a rare disease in which the reduction of the circulating blood cells results from damage to the stem cell pool in bone marrow. In most patients, the stem cell lesion is caused by an autoimmune attack. T-lymphocytes, activated by an endogenous or exogenous, and most often unknown antigenic stimulus, secrete cytokines, including IFN-gamma, which would in turn be able to suppress hematopoiesis. -
Sequence similarities
Belongs to the type II (or gamma) interferon family. -
Post-translational
modificationsProteolytic processing produces C-terminal heterogeneity, with proteins ending alternatively at Gly-150, Met-157 or Gly-161. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 3458 Human
- Omim: 147570 Human
- SwissProt: P01579 Human
- Unigene: 856 Human
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Alternative names
- IF 1 antibody
- IFG antibody
- IFI antibody
see all
Datasheets and documents
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SDS download
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Datasheet download
References (6)
ab9801 has been referenced in 6 publications.
- Liang Z & Tang F The potency of lncRNA MALAT1/miR-155/CTLA4 axis in altering Th1/Th2 balance of asthma. Biosci Rep 40:N/A (2020). PubMed: 31909418
- Hou J et al. PTPROt maintains T cell immunity in the microenvironment of hepatocellular carcinoma. J Mol Cell Biol 7:338-50 (2015). PubMed: 26117839
- Huang H et al. A specific docking site for DNA polymerase {alpha}-primase on the SV40 helicase is required for viral primosome activity, but helicase activity is dispensable. J Biol Chem 285:33475-84 (2010). WB . PubMed: 20685648
- Reddy PK et al. Increased yield of high purity recombinant human interferon-gamma utilizing reversed phase column chromatography. Protein Expr Purif 52:123-30 (2007). PubMed: 17049266
- Poluektova L et al. Immunoregulatory effects of N9-benzyl- and N7-benzyl-8-bromoguanines. Int J Immunopharmacol 21:777-92 (1999). PubMed: 10576622
- Osna N et al. Chronic hepatitis C: T-helper1/T-helper2 imbalance could cause virus persistence in peripheral blood. Scand J Clin Lab Invest 57:703-10 (1997). Sandwich ELISA . PubMed: 9458493