For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Customized Products & Partnerships
    Customized Products & Partnerships

    Customized products and commercial partnerships to accelerate your diagnostic and therapeutic programs.

    Customized products

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

  1. Link

    interferon-gamma-antibody-ifng466-ab218426.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Immunology Innate Immunity Cytokines Interferons
Share by email

Anti-Interferon gamma antibody [IFNG/466] (ab218426)

  • Datasheet
Submit a review Submit a question References (7)

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Shipping info

Promotion Information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Interferon gamma antibody [IFNG/466] (ab218426)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Interferon gamma antibody [IFNG/466] (ab218426)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Interferon gamma antibody [IFNG/466] (ab218426)

Key features and details

  • Mouse monoclonal [IFNG/466] to Interferon gamma
  • Suitable for: IHC-P
  • Reacts with: Human
  • Isotype: IgG2b

You may also be interested in

Primary
Product image
Anti-CD3 antibody [CD3-12] (ab11089)
Primary
Product image
Anti-CD35 antibody [EPR6602] (ab126737)
ELISA
Product image
Human IFN gamma ELISA Kit (ab174443)

View more associated products

Overview

  • Product name

    Anti-Interferon gamma antibody [IFNG/466]
    See all Interferon gamma primary antibodies
  • Description

    Mouse monoclonal [IFNG/466] to Interferon gamma
  • Host species

    Mouse
  • Tested applications

    Suitable for: IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant full length protein within Human Interferon gamma. The exact sequence is proprietary.
    Database link: P01579

  • Positive control

    • IHC-P: Human renal cell carcinoma and testicular carcinoma tissue.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.05% Sodium azide
    Constituents: 99% PBS, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    IFNG/466
  • Isotype

    IgG2b
  • Light chain type

    kappa
  • Research areas

    • Immunology
    • Innate Immunity
    • Cytokines
    • Interferons
    • Stem Cells
    • Signaling Pathways
    • TGF beta
    • Interferon gamma
    • Cancer
    • Tumor immunology
    • Cytokines
    • Interferons
    • Immunology
    • Adaptive Immunity
    • Regulatory T Cells
    • Neuroscience
    • Processes

Associated products

  • Alternative Versions

    • Anti-Interferon gamma antibody [IFNG/466] - BSA and Azide free (ab218890)
  • Isotype control

    • Mouse IgG2b, kappa monoclonal [7E10G10] - Isotype Control (ab170192)
  • Recombinant Protein

    • Recombinant Human Interferon gamma protein (Active) (ab259377)
  • Related Products

    • Recombinant human Interferon gamma protein (Active) (ab9659)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab218426 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Notes
IHC-P
Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target

  • Function

    Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons.
  • Tissue specificity

    Released primarily from activated T lymphocytes.
  • Involvement in disease

    In Caucasians, genetic variation in IFNG is associated with the risk of aplastic anemia (AA) [MIM:609135]. AA is a rare disease in which the reduction of the circulating blood cells results from damage to the stem cell pool in bone marrow. In most patients, the stem cell lesion is caused by an autoimmune attack. T-lymphocytes, activated by an endogenous or exogenous, and most often unknown antigenic stimulus, secrete cytokines, including IFN-gamma, which would in turn be able to suppress hematopoiesis.
  • Sequence similarities

    Belongs to the type II (or gamma) interferon family.
  • Post-translational
    modifications

    Proteolytic processing produces C-terminal heterogeneity, with proteins ending alternatively at Gly-150, Met-157 or Gly-161.
  • Cellular localization

    Secreted.
  • Target information above from: UniProt accession P01579 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 3458 Human
    • Omim: 147570 Human
    • SwissProt: P01579 Human
    • Unigene: 856 Human
    • Alternative names

      • IF 1 antibody
      • IFG antibody
      • IFI antibody
      • ifn g antibody
      • IFN gamma antibody
      • IFN immune antibody
      • IFN, immune antibody
      • IFN-gamma antibody
      • IFNG antibody
      • IFNG_HUMAN antibody
      • Immune interferon antibody
      • Interferon gamma antibody
      • Type II Interferon antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Interferon gamma antibody [IFNG/466] (ab218426)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Interferon gamma antibody [IFNG/466] (ab218426)

      Immunohistochemical analysis of paraffin embedded, formalin fixed human renal cell tissue labeling Interferon gamma using ab218426 at 2 µg/mL.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Interferon gamma antibody [IFNG/466] (ab218426)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Interferon gamma antibody [IFNG/466] (ab218426)

      Immunohistochemical analysis of paraffin embedded, formalin fixed human testicular tissue labeling Interferon gamma using ab218426 at 2 µg/mL.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Interferon gamma antibody [IFNG/466] (ab218426)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Interferon gamma antibody [IFNG/466] (ab218426)

      Immunohistochemical analysis of paraffin embedded, formalin fixed human renal cell tissue labeling Interferon gamma using ab218426 at 2 µg/mL.

    Protocols

    • Immunohistochemistry protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet download

      Download

    References (7)

    Publishing research using ab218426? Please let us know so that we can cite the reference in this datasheet.

    ab218426 has been referenced in 7 publications.

    • Fania L  et al. Paradoxical psoriasis induced by TNF-a blockade shows immunological features typical of the early phase of psoriasis development. J Pathol Clin Res 6:55-68 (2020). PubMed: 31577850
    • Liu Y  et al. Mucus production stimulated by IFN-AhR signaling triggers hypoxia of COVID-19. Cell Res 30:1078-1087 (2020). PubMed: 33159154
    • Fanoni D  et al. Evidence for a role of autoinflammation in early-phase psoriasis. Clin Exp Immunol 198:283-291 (2019). PubMed: 31509228
    • Li YD  et al. Systemic and local immunosuppression in patients with high-grade meningiomas. Cancer Immunol Immunother 68:999-1009 (2019). PubMed: 31030234
    • Ottaiano A  et al. Cetuximab, irinotecan and fluorouracile in fiRst-line treatment of immunologically-selected advanced colorectal cancer patients: the CIFRA study protocol. BMC Cancer 19:899 (2019). PubMed: 31500586
    • van Wageningen TA  et al. Regulation of microglial TMEM119 and P2RY12 immunoreactivity in multiple sclerosis white and grey matter lesions is dependent on their inflammatory environment. Acta Neuropathol Commun 7:206 (2019). PubMed: 31829283
    • Komura T  et al. Clinical features of cystatin A expression in patients with pancreatic ductal adenocarcinoma. Cancer Sci 108:2122-2129 (2017). IHC ; Human . PubMed: 28898495

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    There are currently no Customer reviews or Questions for ab218426.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Get resources and offers direct to your inbox Sign up
    A-Z by research area
    • Cancer
    • Cardiovascular
    • Cell biology
    • Developmental biology
    • Epigenetics & Nuclear signaling
    • Immunology
    • Metabolism
    • Microbiology
    • Neuroscience
    • Signal transduction
    • Stem cells
    A-Z by product type
    • Primary antibodies
    • Secondary antibodies
    • Biochemicals
    • Isotype controls
    • Flow cytometry multi-color selector
    • Kits
    • Loading controls
    • Lysates
    • Peptides
    • Proteins
    • Slides
    • Tags and cell markers
    • Tools & Reagents
    Help & support
    • Support
    • Make an Inquiry
    • Protocols & troubleshooting
    • Placing an order
    • RabMAb products
    • Biochemical product FAQs
    • Training
    • Browse by Target
    Company
    • Corporate site
    • Investor relations
    • Company news
    • Careers
    • About us
    • Blog
    Events
    • Tradeshows
    • Conferences
    International websites
    • abcam.cn
    • abcam.co.jp

    Join with us

    • LinkedIn
    • facebook
    • Twitter
    • YouTube
    • Terms of sale
    • Website terms of use
    • Cookie policy
    • Privacy policy
    • Legal
    • Modern slavery statement
    © 1998-2022 Abcam plc. All rights reserved.