Anti-Interferon gamma antibody [IFNG/466] (ab218426)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Interferon gamma antibody [IFNG/466] (ab218426)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Interferon gamma antibody [IFNG/466] (ab218426)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Interferon gamma antibody [IFNG/466] (ab218426)

Key features and details

  • Mouse monoclonal [IFNG/466] to Interferon gamma
  • Suitable for: IHC-P
  • Reacts with: Human
  • Isotype: IgG2b

Overview

  • Product name

    Anti-Interferon gamma antibody [IFNG/466]
    See all Interferon gamma primary antibodies

  • Description

    Mouse monoclonal [IFNG/466] to Interferon gamma

  • Host species

    Mouse

  • Tested applications

    Suitable for: IHC-Pmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant full length protein within Human Interferon gamma. The exact sequence is proprietary.
    Database link: P01579

  • Positive control

    • IHC-P: Human renal cell carcinoma and testicular carcinoma tissue.

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab218426 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Notes
IHC-P
Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target

  • Function

    Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons.

  • Tissue specificity

    Released primarily from activated T lymphocytes.

  • Involvement in disease

    In Caucasians, genetic variation in IFNG is associated with the risk of aplastic anemia (AA) [MIM:609135]. AA is a rare disease in which the reduction of the circulating blood cells results from damage to the stem cell pool in bone marrow. In most patients, the stem cell lesion is caused by an autoimmune attack. T-lymphocytes, activated by an endogenous or exogenous, and most often unknown antigenic stimulus, secrete cytokines, including IFN-gamma, which would in turn be able to suppress hematopoiesis.

  • Sequence similarities

    Belongs to the type II (or gamma) interferon family.

  • Post-translational
    modifications

    Proteolytic processing produces C-terminal heterogeneity, with proteins ending alternatively at Gly-150, Met-157 or Gly-161.

  • Cellular localization

    Secreted.
  • Information by UniProt

  • Database links

    • Alternative names

      • IF 1 antibody
      • IFG antibody
      • IFI antibody
      • ifn g antibody
      • IFN gamma antibody
      • IFN immune antibody
      • IFN, immune antibody
      • IFN-gamma antibody
      • IFNG antibody
      • IFNG_HUMAN antibody
      • Immune interferon antibody
      • Interferon gamma antibody
      • Type II Interferon antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Interferon gamma antibody [IFNG/466] (ab218426)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Interferon gamma antibody [IFNG/466] (ab218426)

      Immunohistochemical analysis of paraffin embedded, formalin fixed human renal cell tissue labeling Interferon gamma using ab218426 at 2 µg/mL.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Interferon gamma antibody [IFNG/466] (ab218426)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Interferon gamma antibody [IFNG/466] (ab218426)

      Immunohistochemical analysis of paraffin embedded, formalin fixed human testicular tissue labeling Interferon gamma using ab218426 at 2 µg/mL.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Interferon gamma antibody [IFNG/466] (ab218426)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Interferon gamma antibody [IFNG/466] (ab218426)

      Immunohistochemical analysis of paraffin embedded, formalin fixed human renal cell tissue labeling Interferon gamma using ab218426 at 2 µg/mL.

    References (7)

    Publishing research using ab218426? Please let us know so that we can cite the reference in this datasheet.

    ab218426 has been referenced in 7 publications.

    • Fania L  et al. Paradoxical psoriasis induced by TNF-a blockade shows immunological features typical of the early phase of psoriasis development. J Pathol Clin Res 6:55-68 (2020). PubMed: 31577850
    • Liu Y  et al. Mucus production stimulated by IFN-AhR signaling triggers hypoxia of COVID-19. Cell Res 30:1078-1087 (2020). PubMed: 33159154
    • Fanoni D  et al. Evidence for a role of autoinflammation in early-phase psoriasis. Clin Exp Immunol 198:283-291 (2019). PubMed: 31509228
    • Li YD  et al. Systemic and local immunosuppression in patients with high-grade meningiomas. Cancer Immunol Immunother 68:999-1009 (2019). PubMed: 31030234
    • Ottaiano A  et al. Cetuximab, irinotecan and fluorouracile in fiRst-line treatment of immunologically-selected advanced colorectal cancer patients: the CIFRA study protocol. BMC Cancer 19:899 (2019). PubMed: 31500586
    • van Wageningen TA  et al. Regulation of microglial TMEM119 and P2RY12 immunoreactivity in multiple sclerosis white and grey matter lesions is dependent on their inflammatory environment. Acta Neuropathol Commun 7:206 (2019). PubMed: 31829283
    • Komura T  et al. Clinical features of cystatin A expression in patients with pancreatic ductal adenocarcinoma. Cancer Sci 108:2122-2129 (2017). IHC ; Human . PubMed: 28898495

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