Anti-Interferon gamma antibody [MD-1] (ab25014)

Reviews (1)Q&A (1)

Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab25014 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.

Use at an assay dependent dilution.
ELISpot Use at an assay dependent concentration.

Use at an assay dependent dilution.
Neutralising Use at an assay dependent concentration.

Antibody activity is 15,000 neutralizing units/mg protein. One neutralizing unit is defined as the total amount of antibodies sufficient for neutralizing one antiviral unit of human IFN-gamma as calibrated against NIH standard Gg 23-901-530.
WB Use at an assay dependent concentration. Predicted molecular weight: 19 kDa.

Use at an assay dependent dilution.
Sandwich ELISA Use a concentration of 5 µg/ml. Can be paired for Sandwich ELISA with Rabbit polyclonal to Interferon gamma (ab25101).

For sandwich ELISA, use this antibody as Capture at 5µg/ml with ab25101 as Detection.
Flow Cyt Use at an assay dependent concentration.

Good for intracellular flow cytometric staining. Use at an assay dependent dilution.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
IHC-Fr Use at an assay dependent concentration.

Use at an assay dependent dilution.

Target

  • Function
    Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons.
  • Tissue specificity
    Released primarily from activated T lymphocytes.
  • Involvement in disease
    In Caucasians, genetic variation in IFNG is associated with the risk of aplastic anemia (AA) [MIM:609135]. AA is a rare disease in which the reduction of the circulating blood cells results from damage to the stem cell pool in bone marrow. In most patients, the stem cell lesion is caused by an autoimmune attack. T-lymphocytes, activated by an endogenous or exogenous, and most often unknown antigenic stimulus, secrete cytokines, including IFN-gamma, which would in turn be able to suppress hematopoiesis.
  • Sequence similarities
    Belongs to the type II (or gamma) interferon family.
  • Post-translational
    modifications
    Proteolytic processing produces C-terminal heterogeneity, with proteins ending alternatively at Gly-150, Met-157 or Gly-161.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
    • Alternative names
      • IFG antibody
      • IFI antibody
      • IFN gamma antibody
      • IFN, immune antibody
      • IFN-gamma antibody
      • IFNG antibody
      • IFNG_HUMAN antibody
      • Immune interferon antibody
      • Interferon gamma antibody
      see all

    Images

    • Sandwich ELISA - Anti-Interferon gamma antibody [MD-1] (ab25014)
      Sandwich ELISA - Anti-Interferon gamma antibody [MD-1] (ab25014)
      Standard curve for Interferon gamma (Analyte: ab51240); dilution range 1pg/ml to 1µg/ml using Capture Antibody ab25014 at 5µg/ml and Detector Antibody ab25101 at 0.5µg/ml.

    References

    ab25014 has not yet been referenced specifically in any publications.

    Publishing research using ab25014? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    1-2 of 2 Abreviews or Q&A

    Question

    Dear Sir/Madam, Our customer would like to inquire whether ab25014 and ab25101 have the larger customerized package, for example, 500ug or 1mg. Would you please help us provide this information? Thanks and best regards,

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    Answer

    Thank you for contacting us. We can certainly provide these in 500ug or 1mg size as a special request. Please let me know if you would like to place an order. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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    ELISA abreview for Anti-Interferon gamma antibody [MD-1]

     Good
    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    ELISA
    Sample
    Human Purified protein (Plasma)
    Specification
    Plasma
    Blocking step
    BSA as blocking agent for 4 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
    Type
    Direct
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    Ms. Angel Morrow

    Verified customer

    Submitted Jul 26 2007

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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