Anti-Interferon gamma antibody [MD-1] (ab25014)
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Overview
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Product name
Anti-Interferon gamma antibody [MD-1]
See all Interferon gamma primary antibodies -
Description
Mouse monoclonal [MD-1] to Interferon gamma -
Host species
Mouse -
Specificity
The antibody recognises IFN-gamma but does not react IFN-alpha and beta. -
Tested applications
Suitable for: ELISA, ELISpot, Neutralising, WB, Sandwich ELISA, Flow Cyt, IHC-Frmore details -
Species reactivity
Reacts with: Human, Monkey
Does not react with: Mouse, Rat -
Immunogen
Recombinant full length protein (Human)
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Positive control
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: PBS, 4.275% Trehalose -
Concentration information loading...
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Purity
Ion Exchange Chromatography -
Clonality
Monoclonal -
Clone number
MD-1 -
Isotype
IgG1 -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
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sELISA pair antibody
Applications
Our Abpromise guarantee covers the use of ab25014 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ELISA | Use at an assay dependent concentration. Use at an assay dependent dilution. |
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ELISpot | Use at an assay dependent concentration. Use at an assay dependent dilution. |
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Neutralising | Use at an assay dependent concentration. Antibody activity is 15,000 neutralizing units/mg protein. One neutralizing unit is defined as the total amount of antibodies sufficient for neutralizing one antiviral unit of human IFN-gamma as calibrated against NIH standard Gg 23-901-530. |
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WB | Use at an assay dependent concentration. Predicted molecular weight: 19 kDa. Use at an assay dependent dilution. |
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Sandwich ELISA | Use a concentration of 5 µg/ml. Can be paired for Sandwich ELISA with Rabbit polyclonal to Interferon gamma (ab25101). For sandwich ELISA, use this antibody as Capture at 5µg/ml with ab25101 as Detection. |
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Flow Cyt | Use at an assay dependent concentration. Good for intracellular flow cytometric staining. Use at an assay dependent dilution. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
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IHC-Fr | Use at an assay dependent concentration. Use at an assay dependent dilution. |
Target
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Function
Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons. -
Tissue specificity
Released primarily from activated T lymphocytes. -
Involvement in disease
In Caucasians, genetic variation in IFNG is associated with the risk of aplastic anemia (AA) [MIM:609135]. AA is a rare disease in which the reduction of the circulating blood cells results from damage to the stem cell pool in bone marrow. In most patients, the stem cell lesion is caused by an autoimmune attack. T-lymphocytes, activated by an endogenous or exogenous, and most often unknown antigenic stimulus, secrete cytokines, including IFN-gamma, which would in turn be able to suppress hematopoiesis. -
Sequence similarities
Belongs to the type II (or gamma) interferon family. -
Post-translational
modificationsProteolytic processing produces C-terminal heterogeneity, with proteins ending alternatively at Gly-150, Met-157 or Gly-161. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 3458 Human
- Omim: 147570 Human
- SwissProt: P01579 Human
- Unigene: 856 Human
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Alternative names
- IF 1 antibody
- IFG antibody
- IFI antibody
see all
Images
Protocols
References
ab25014 has not yet been referenced specifically in any publications.