Anti-Interferon gamma antibody [MD-1] (ab25014)

Reviews (1)Q&A (1)
Sandwich ELISA - Anti-Interferon gamma antibody [MD-1] (ab25014)

    Key features and details

    • Mouse monoclonal [MD-1] to Interferon gamma
    • Suitable for: Sandwich ELISA
    • Reacts with: Human
    • Isotype: IgG1

    Overview

    • Product name

      Anti-Interferon gamma antibody [MD-1]
      See all Interferon gamma primary antibodies

    • Description

      Mouse monoclonal [MD-1] to Interferon gamma

    • Host species

      Mouse

    • Specificity

      The antibody recognises IFN-gamma but does not react IFN-alpha and beta.

    • Tested applications

      Suitable for: Sandwich ELISAmore details

    • Species reactivity

      Reacts with: Human
      Does not react with: Mouse, Rat

    • Immunogen

      Recombinant full length protein (Human)

    • Positive control

    • General notes

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

      One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

      Learn more here.

    Properties

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab25014 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Sandwich ELISA
    Use a concentration of 5 µg/ml. Can be paired for Sandwich ELISA with Rabbit polyclonal to Interferon gamma (ab25101).

    For sandwich ELISA, use this antibody as Capture at 5µg/ml with ab25101 as Detection.
    Notes
    Sandwich ELISA
    Use a concentration of 5 µg/ml. Can be paired for Sandwich ELISA with Rabbit polyclonal to Interferon gamma (ab25101).

    For sandwich ELISA, use this antibody as Capture at 5µg/ml with ab25101 as Detection.

    Target

    • Function

      Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons.

    • Tissue specificity

      Released primarily from activated T lymphocytes.

    • Involvement in disease

      In Caucasians, genetic variation in IFNG is associated with the risk of aplastic anemia (AA) [MIM:609135]. AA is a rare disease in which the reduction of the circulating blood cells results from damage to the stem cell pool in bone marrow. In most patients, the stem cell lesion is caused by an autoimmune attack. T-lymphocytes, activated by an endogenous or exogenous, and most often unknown antigenic stimulus, secrete cytokines, including IFN-gamma, which would in turn be able to suppress hematopoiesis.

    • Sequence similarities

      Belongs to the type II (or gamma) interferon family.

    • Post-translational
      modifications

      Proteolytic processing produces C-terminal heterogeneity, with proteins ending alternatively at Gly-150, Met-157 or Gly-161.

    • Cellular localization

      Secreted.
    • Information by UniProt

    • Database links

      • Alternative names

        • IF 1 antibody
        • IFG antibody
        • IFI antibody
        • IFN gamma antibody
        • IFN immune antibody
        • IFN, immune antibody
        • IFN-gamma antibody
        • IFNG antibody
        • IFNG_HUMAN antibody
        • Immune interferon antibody
        • Interferon gamma antibody
        • Type II Interferon antibody
        see all

      Images

      • Sandwich ELISA - Anti-Interferon gamma antibody [MD-1] (ab25014)
        Sandwich ELISA - Anti-Interferon gamma antibody [MD-1] (ab25014)
        Standard curve for Interferon gamma (Analyte: ab51240); dilution range 1pg/ml to 1µg/ml using Capture Antibody ab25014 at 5µg/ml and Detector Antibody ab25101 at 0.5µg/ml.

      References (0)

      Publishing research using ab25014? Please let us know so that we can cite the reference in this datasheet.

      ab25014 has not yet been referenced specifically in any publications.

      Customer reviews and Q&As

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      1-2 of 2 Abreviews or Q&A

      Question

      Dear Sir/Madam, Our customer would like to inquire whether ab25014 and ab25101 have the larger customerized package, for example, 500ug or 1mg. Would you please help us provide this information? Thanks and best regards,

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      Answer

      Thank you for contacting us. We can certainly provide these in 500ug or 1mg size as a special request. Please let me know if you would like to place an order. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

      Read More

      ELISA abreview for Anti-Interferon gamma antibody [MD-1]

       Good
      Abreviews
      Application
      ELISA
      Sample
      Human Purified protein (Plasma)
      Specification
      Plasma
      Blocking step
      BSA as blocking agent for 4 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
      Type
      Direct
      Read More

      Ms. Angel Morrow

      Verified customer

      Submitted Jul 26 2007

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