Anti-Interferon gamma antibody [MD-1] (ab25014)
Key features and details
- Mouse monoclonal [MD-1] to Interferon gamma
- Suitable for: Sandwich ELISA
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-Interferon gamma antibody [MD-1]
See all Interferon gamma primary antibodies -
Description
Mouse monoclonal [MD-1] to Interferon gamma -
Host species
Mouse -
Specificity
The antibody recognises IFN-gamma but does not react IFN-alpha and beta. -
Tested applications
Suitable for: Sandwich ELISAmore details -
Species reactivity
Reacts with: Human
Does not react with: Mouse, Rat -
Immunogen
Recombinant full length protein (Human)
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: PBS, 4.275% Trehalose -
Concentration information loading...
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Purity
Ion Exchange Chromatography -
Clonality
Monoclonal -
Clone number
MD-1 -
Isotype
IgG1 -
Research areas
Associated products
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Conjugation kits
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Isotype control
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Recombinant Protein
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sELISA pair antibody
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab25014 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Sandwich ELISA |
Use a concentration of 5 µg/ml. Can be paired for Sandwich ELISA with Rabbit polyclonal to Interferon gamma (ab25101).
For sandwich ELISA, use this antibody as Capture at 5µg/ml with ab25101 as Detection. |
Notes |
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Sandwich ELISA
Use a concentration of 5 µg/ml. Can be paired for Sandwich ELISA with Rabbit polyclonal to Interferon gamma (ab25101). For sandwich ELISA, use this antibody as Capture at 5µg/ml with ab25101 as Detection. |
Target
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Function
Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons. -
Tissue specificity
Released primarily from activated T lymphocytes. -
Involvement in disease
In Caucasians, genetic variation in IFNG is associated with the risk of aplastic anemia (AA) [MIM:609135]. AA is a rare disease in which the reduction of the circulating blood cells results from damage to the stem cell pool in bone marrow. In most patients, the stem cell lesion is caused by an autoimmune attack. T-lymphocytes, activated by an endogenous or exogenous, and most often unknown antigenic stimulus, secrete cytokines, including IFN-gamma, which would in turn be able to suppress hematopoiesis. -
Sequence similarities
Belongs to the type II (or gamma) interferon family. -
Post-translational
modificationsProteolytic processing produces C-terminal heterogeneity, with proteins ending alternatively at Gly-150, Met-157 or Gly-161. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 3458 Human
- Omim: 147570 Human
- SwissProt: P01579 Human
- Unigene: 856 Human
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Alternative names
- IF 1 antibody
- IFG antibody
- IFI antibody
see all
Images
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab25014 has been referenced in 2 publications.
- Chua KV et al. Octyl Gallate Induces Pancreatic Ductal Adenocarcinoma Cell Apoptosis and Suppresses Endothelial-Mesenchymal Transition-Promoted M2-Macrophages, HSP90a Secretion, and Tumor Growth. Cells 9:N/A (2019). PubMed: 31905895
- Aebischer J et al. Elevated levels of IFNγ and LIGHT in the spinal cord of patients with sporadic amyotrophic lateral sclerosis. Eur J Neurol 19:752-9, e45-6 (2012). PubMed: 22221541