Validated using a knockout cell line
Recombinant
RabMAb

Anti-Interferon Receptor alpha antibody [EPR6244] (ab124764)

Overview

  • Product name
    Anti-Interferon Receptor alpha antibody [EPR6244]
    See all Interferon Receptor alpha primary antibodies
  • Description
    Rabbit monoclonal [EPR6244] to Interferon Receptor alpha
  • Host species
    Rabbit
  • Specificity
    In mouse and rat tissue lysates this product detects a band in the region of 100 kDa, however we believe this band is non-specific and is not interferon receptor alpha as the immunogen for this antibody shares only 55% homology with the mouse and rat protein. In addition this band also migrates at a lower molecular weight than that detected in human samples.
  • Tested applications
    Suitable for: WBmore details
    Unsuitable for: ICC/IF or IHC-P
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Interferon Receptor alpha aa 100-200. The exact sequence is proprietary.

  • Positive control
    • HeLa, HeLa treated with IFN-alpha, K562 and U937 cell lysates.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
  • Dissociation constant (KD)
    KD = 2.40 x 10 -12 M
    Learn more about KD
  • Storage buffer
    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 50% Glycerol, 0.05% BSA, 49% PBS
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Monoclonal
  • Clone number
    EPR6244
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab124764 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 90-130 kDa (predicted molecular weight: 64 kDa).
  • Application notes
    Is unsuitable for ICC/IF or IHC-P.
  • Target

    • Relevance
      Interferon Receptor alpha (IFN-a R1) is a class II cytokine receptor which belongs to type I human interferons (IFNs) family. IFNs plays a role in antiviral, antiproliferative, immunomodulatory, antitumor, and antiparasitic activities by inducing transcription of IFN-stimuated genes (ISGs) through activation of the Jak-STAT pathway. Specifically, Interferon Receptor alpha is a 135-kda signal transducing subunit of the IFN alpha/beta receptor complex (IFN-a R). Interferon Receptor alpha is phospholyated through PTK- and PKC-mediated phosphorylation, which initiates docking of STAT proteins to IFNs. This leads to activation of STAT proteins by PTK-mediated phosphorylation. The mechanism behind the amplification of Interferon Receptor alpha complex mediated transmembrane signaling has been linked to tyrosine phosphorylation on Interferon Receptor alpha. IFNAR1 possesses a large N-glycosylated ectodomain.
    • Cellular localization
      Membrane; single-pass type I membrane protein.
    • Database links
    • Alternative names
      • AVP antibody
      • IFN alpha R1 antibody
      • IFN alpha REC antibody
      • IFNAR antibody
      • IFNAR1 antibody
      • IFNBR antibody
      • IFRC antibody
      • interferon (alpha beta and omega) receptor 1 antibody
      • Interferon alpha receptor 1 antibody
      • Interferon alpha/beta receptor alpha chain antibody
      see all

    Images

    • All lanes : Anti-Interferon Receptor alpha antibody [EPR6244] (ab124764) at 1/1000 dilution

      Lane 1 : Wild-type HeLa whole cell lysate
      Lane 2 : IFNAR1 (Interferon Receptor alpha) knockout HeLa whole cell lysate
      Lane 3 : HepG2 whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Predicted band size: 64 kDa



      Lanes 1 - 3: Merged signal (red and green). Green - ab124764 observed at 90 kDa. Red - loading control, ab9484, observed at 37 kDa.

      ab124764 was shown to specifically react with Interferon Receptor alpha in wild-type HeLa cells as signal was lost in IFNAR1 (Interferon Receptor alpha) knockout cells. Wild-type and IFNAR1 (Interferon Receptor alpha) knockout samples were subjected to SDS-PAGE. Ab124764 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

    • All lanes : Anti-Interferon Receptor alpha antibody [EPR6244] (ab124764) at 1/1000 dilution (purified)

      Lane 1 : Mouse brain lysate
      Lane 2 : Rat brain lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Rabbit monoclonal [EPR6244] to Interferon Receptor alpha (ab124764) at 1/1000 dilution

      Predicted band size: 64 kDa
      Observed band size: 90 kDa (why is the actual band size different from the predicted?)



      Blocking buffer: 5% NFDM/TBST
      Dilution buffer: 5% NFDM/TBST

      In mouse and rat tissue lysates this product detects a band in the region of 100 kDa, however we believe this band is non-specific and is not interferon receptor alpha as the immunogen for this antibody shares only 55% homology with the mouse and rat protein. In addition this band also migrates at a lower molecular weight than that detected in human samples.

    • All lanes : Anti-Interferon Receptor alpha antibody [EPR6244] (ab124764) at 1/1000 dilution (purified)

      Lane 1 : HEK293 whole cell lysate
      Lane 2 : HeLa whole cell lysate
      Lane 3 : LNCaP whole cell lysate
      Lane 4 : SH-SY5Y whole cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 64 kDa
      Observed band size: 90-130 kDa (why is the actual band size different from the predicted?)



      Blocking buffer: 5% NFDM/TBST
      Dilution buffer: 5% NFDM/TBST

    • All lanes : Anti-Interferon Receptor alpha antibody [EPR6244] (ab124764) at 1/1000 dilution (unpurified)

      Lane 1 : Mouse brain whole cell lysate
      Lane 2 : Mouse heart whole cell lysate
      Lane 3 : Mouse kidney whole cell lysate
      Lane 4 : Mouse spleen whole cell lysate
      Lane 5 : Rat brain whole cell lysate
      Lane 6 : Rat heart whole cell lysate
      Lane 7 : Rat kidney whole cell lysate
      Lane 8 : Rat spleen whole cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit IgG (H+L), peroxidase conjugated. at 1/2000 dilution

      Predicted band size: 64 kDa


      Exposure time: 3 minutes


      Blocking buffer: 5% NFDM/TBST.

      In mouse and rat tissue lysates this product detects a band in the region of 100 kDa, however we believe this band is non-specific and is not interferon receptor alpha as the immunogen for this antibody shares only 55% homology with the mouse and rat protein. In addition this band also migrates at a lower molecular weight than that detected in human samples.

    • All lanes : Anti-Interferon Receptor alpha antibody [EPR6244] (ab124764) at 1/1000 dilution (Unpurified)

      Lane 1 : HeLa cell lysate
      Lane 2 : HeLa cell lysate treated with IFN-alpha
      Lane 3 : K562 cell lysate
      Lane 4 : U937 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-Rabbit HRP at 1/200 dilution

      Predicted band size: 64 kDa

    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    This product has been referenced in:
    • Chmiest D  et al. Spatiotemporal control of interferon-induced JAK/STAT signalling and gene transcription by the retromer complex. Nat Commun 7:13476 (2016). Read more (PubMed: 27917878) »

    See 1 Publication for this product

    Customer reviews and Q&As

    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (human retinal epithelioïd cells)
    Specification
    human retinal epithelioïd cells
    Fixative
    Paraformaldehyde
    Permeabilization
    No
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 0.02% · Temperature: RT°C
    Username

    Abcam user community

    Verified customer

    Submitted May 03 2013

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (hTERT-RPE1)
    Loading amount
    150000 cells
    Specification
    hTERT-RPE1
    Gel Running Conditions
    Reduced Denaturing (8 % acrylamid)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Username

    Abcam user community

    Verified customer

    Submitted Apr 16 2013

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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