Anti-Interferon regulatory factor 9/IRF-9 antibody (ab126940)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-Interferon regulatory factor 9/IRF-9 antibody
See all Interferon regulatory factor 9/IRF-9 primary antibodies -
Description
Rabbit polyclonal to Interferon regulatory factor 9/IRF-9 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human Interferon regulatory factor 9/IRF-9 aa 171-354.
Database link: Q00978 -
Positive control
- A549, HeLa, HepG2 and HCT116 whole cell lysates; HeLa cells; Human Breast carcinoma tissue.
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General notes
Keep as concentrated solution.Previously labelled as Interferon regulatory factor 9.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab126940 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Predicted molecular weight: 44 kDa. | |
| IHC-P | 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| ICC/IF | 1/100 - 1/1000. |
Target
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Function
Transcription regulatory factor that mediates signaling by type I IFNs (IFN-alpha and IFN-beta). Following type I IFN binding to cell surface receptors, Jak kinases (TYK2 and JAK1) are activated, leading to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize, associate with IRF9/ISGF3G to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of interferon stimulated genes, which drive the cell in an antiviral state. -
Sequence similarities
Belongs to the IRF family.
Contains 1 IRF tryptophan pentad repeat DNA-binding domain. -
Cellular localization
Cytoplasm. Nucleus. Translocated into the nucleus upon activation by IFN-alpha/beta. - Information by UniProt
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Database links
- Entrez Gene: 10379 Human
- Omim: 147574 Human
- SwissProt: Q00978 Human
- Unigene: 1706 Human
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Alternative names
- IFN alpha responsive transcription factor subunit antibody
- IFN-alpha-responsive transcription factor subunit antibody
- Interferon regulatory factor 9 antibody
see all
Images
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Western blot - Anti-Interferon regulatory factor 9/IRF-9 antibody (ab126940)All lanes : Anti-Interferon regulatory factor 9/IRF-9 antibody (ab126940) at 1/5000 dilution
Lane 1 : A549 whole cell lysate
Lane 2 : HeLa whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 44 kDa
10% SDS PAGE -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Interferon regulatory factor 9/IRF-9 antibody (ab126940)ab126940, at 1/250, staining Interferon regulatory factor 9/IRF-9 in paraffin-embedded Human Breast carcinoma tissue by Immunohistochemistry.
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Immunocytochemistry/ Immunofluorescence - Anti-Interferon regulatory factor 9/IRF-9 antibody (ab126940)ab126940, at 1/500, staining Interferon regulatory factor 9/IRF-9 in paraformaldehyde-fixed HeLa cells by confocal immunofluorescence. Alpha-tubulin filaments are stained red.
Protocols
References
ab126940 has not yet been referenced specifically in any publications.
