Key features and details
- Rabbit polyclonal to Interferon regulatory factor 9/IRF-9
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Interferon regulatory factor 9/IRF-9 antibody
DescriptionRabbit polyclonal to Interferon regulatory factor 9/IRF-9
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Human
Recombinant fragment corresponding to Human Interferon regulatory factor 9/IRF-9 aa 171-354.
Database link: Q00978
- A549, HeLa, HepG2 and HCT116 whole cell lysates; HeLa cells; Human Breast carcinoma tissue.
General notesKeep as concentrated solution.
Previously labelled as Interferon regulatory factor 9.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab126940 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 44 kDa.|
|IHC-P||1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||1/100 - 1/1000.|
FunctionTranscription regulatory factor that mediates signaling by type I IFNs (IFN-alpha and IFN-beta). Following type I IFN binding to cell surface receptors, Jak kinases (TYK2 and JAK1) are activated, leading to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize, associate with IRF9/ISGF3G to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of interferon stimulated genes, which drive the cell in an antiviral state.
Sequence similaritiesBelongs to the IRF family.
Contains 1 IRF tryptophan pentad repeat DNA-binding domain.
Cellular localizationCytoplasm. Nucleus. Translocated into the nucleus upon activation by IFN-alpha/beta.
- Information by UniProt
- IFN alpha responsive transcription factor subunit antibody
- IFN-alpha-responsive transcription factor subunit antibody
- Interferon regulatory factor 9 antibody
ab126940, at 1/250, staining Interferon regulatory factor 9/IRF-9 in paraffin-embedded Human Breast carcinoma tissue by Immunohistochemistry.
All lanes : Anti-Interferon regulatory factor 9/IRF-9 antibody (ab126940) at 1/5000 dilution
Lane 1 : A549 whole cell lysate
Lane 2 : HeLa whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 44 kDa
10% SDS PAGE
ab126940, at 1/500, staining Interferon regulatory factor 9/IRF-9 in paraformaldehyde-fixed HeLa cells by confocal immunofluorescence. Alpha-tubulin filaments are stained red.
ab126940 has not yet been referenced specifically in any publications.