Anti-Interferon regulatory factor 9/IRF-9 antibody (ab126940)
Key features and details
- Rabbit polyclonal to Interferon regulatory factor 9/IRF-9
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Interferon regulatory factor 9/IRF-9 antibody -
Description
Rabbit polyclonal to Interferon regulatory factor 9/IRF-9 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human Interferon regulatory factor 9/IRF-9 aa 171-354.
Database link: Q00978 -
Positive control
- A549, HeLa, HepG2 and HCT116 whole cell lysates; HeLa cells; Human Breast carcinoma tissue.
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General notes
Keep as concentrated solution.Previously labelled as Interferon regulatory factor 9.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab126940 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Predicted molecular weight: 44 kDa. | |
| IHC-P | 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| ICC/IF | 1/100 - 1/1000. |
Target
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Function
Transcription regulatory factor that mediates signaling by type I IFNs (IFN-alpha and IFN-beta). Following type I IFN binding to cell surface receptors, Jak kinases (TYK2 and JAK1) are activated, leading to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize, associate with IRF9/ISGF3G to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of interferon stimulated genes, which drive the cell in an antiviral state. -
Sequence similarities
Belongs to the IRF family.
Contains 1 IRF tryptophan pentad repeat DNA-binding domain. -
Cellular localization
Cytoplasm. Nucleus. Translocated into the nucleus upon activation by IFN-alpha/beta. - Information by UniProt
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Database links
- Entrez Gene: 10379 Human
- Omim: 147574 Human
- SwissProt: Q00978 Human
- Unigene: 1706 Human
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Alternative names
- IFN alpha responsive transcription factor subunit antibody
- IFN-alpha-responsive transcription factor subunit antibody
- Interferon regulatory factor 9 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Interferon regulatory factor 9/IRF-9 antibody (ab126940)ab126940, at 1/250, staining Interferon regulatory factor 9/IRF-9 in paraffin-embedded Human Breast carcinoma tissue by Immunohistochemistry.
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Western blot - Anti-Interferon regulatory factor 9/IRF-9 antibody (ab126940)All lanes : Anti-Interferon regulatory factor 9/IRF-9 antibody (ab126940) at 1/5000 dilution
Lane 1 : A549 whole cell lysate
Lane 2 : HeLa whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 44 kDa
10% SDS PAGE -
Immunocytochemistry/ Immunofluorescence - Anti-Interferon regulatory factor 9/IRF-9 antibody (ab126940)ab126940, at 1/500, staining Interferon regulatory factor 9/IRF-9 in paraformaldehyde-fixed HeLa cells by confocal immunofluorescence. Alpha-tubulin filaments are stained red.
Protocols
References (0)
ab126940 has not yet been referenced specifically in any publications.
