Product nameIntestinal Permeability Assay Kit
Intestinal Permeability Assay Kit (ab272540) is based on measuring the ratio of the absorption of two non-metabolized sugars through the intestines. Test subjects drink a prescribed amount of lactulose and mannitol and the % absorption of these sugars is determined by the amount of excreted lactulose and mannitol measured during the first 6 hours after ingestion. The degree of intestinal permeability is reflected by the ratio of the % absorption of lactulose to % absorption of mannitol. An increase in this ratio indicates increased intestinal permeability since lactulose is only absorbed though intercellular spaces. Lactulose and mannitol are measured in separate assays using the included Lactulose Assay Kit and Mannitol Assay Kit, respectively.
Simple and convenient: Both assays require addition of single working reagent and can be completed within 60 minutes. Both assays are performed at room temperature. No 37°C heater is needed.
High-throughput: Can be readily automated as a high-throughput 96- well plate assay for thousands of samples per day.
Detection Limit: Mannitol - 7 μM; Lactulose - 3 μM.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Tested applicationsSuitable for: Functional Studiesmore details
Storage instructionsStore at -20°C. Please refer to protocols.
Components 100 tests Lactulose Assay Buffer 1 x 6ml Lactulose Enzyme A 1 vial Lactulose Enzyme B 1 x 120µl Lactulose Enzyme Buffer 1 x 150µl Lactulose PMS Solution 1 x 1.5ml Lactulose Standard 1 x 400µl Mannitiol Standard 1 x 500µl Mannitol Assay Buffer 1 x 10ml Mannitol Enzyme 1 x 120µl
Our Abpromise guarantee covers the use of ab272540 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent concentration.|
ab272540 has not yet been referenced specifically in any publications.