Intracellular glutathione (GSH) Detection Assay Kit (ab112132)


  • Product name
    Intracellular glutathione (GSH) Detection Assay Kit
    See all Glutathione kits
  • Sample type
    Adherent cells, Suspension cells
  • Product overview

    Intracellular glutathione (GSH) Detection Assay Kit (ab112132) uses our proprietary non-fluorescent Green Dye, which becomes strongly fluorescent upon reacting with thiol (including GSH in cells). In normal cells, the Green Dye is accumulated primarily in cytosol, but it is partially translocated to mitochondria in apoptotic cells while Green Dye staining intensity is decreased. Cells stained with Green Dye can be visualized with a flow cytometer at Ex/Em = 490/520 nm (FL1 channel).
    This kit has been designed to detect apoptosis is cells by measuring the decrease in reduced glutathione (GSH), which is an early hallmark in the progression of cell death in response to different apoptotic stimuli in many cells.

    This kit can be used together with other reagents, such as 7-AAD for multi-parametric study of cell viability and apoptosis. The kit is optimized for screening apoptosis activators and inhibitors with a flow cytometer.


    We do not recommend using this kit in a microplate. A more suitable option might be ab65322.


    Visit our FAQs page for tips and troubleshooting.

  • Notes

    Glutathione is a tripeptide that contains L-cysteine, L-glutamic acid and glycine. It is the smallest intracellular protein thiol molecule in the cells, which prevents cell damage caused by reactive oxygen species such as free radicals and peroxides. Glutathione exists in reduced (GSH) and oxidized (GSSG) states. Reduced glutathione (GSH) is a major tissue antioxidant that provides reducing equivalents for the glutathione peroxidase (GPx) catalyzed reduction of lipid hydroperoxides to their corresponding alcohols and hydrogen peroxide to water. In the GPx catalyzed reaction, the formation of a disulfide bond between two GSH molecules generates oxidized glutathione (GSSG). The enzyme glutathione reductase (GR) recycles GSSG to GSH with the simultaneous oxidation of β-nicotinamide adenine dinucleotide phosphate (β-NADPH2). In healthy cells, more than 90% of the total glutathione pool is in the reduced form (GSH). When cells are exposed to increased levels of oxidative stress, GSSG accumulates and the ratio of GSSG to GSH increases. An increased ratio of GSSG-to-GSH is an indication of oxidative stress. The monitoring of reduced and oxidized GSH in biological samples is essential for evaluating the redox and detoxification status of the cells and tissues against oxidative and free radicals mediated cell injury.

  • Tested applications
    Suitable for: Flow Cytmore details


Associated products


Our Abpromise guarantee covers the use of ab112132 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration.


  • Decrease in the fluorescence intensity of the Thiol Green Dye correlates with the addition of camptothecin in Jurkat cells. Jurkat cells were mock induced with 0.25% DMSO (blue line) and with 12.5 μM camptothecin (black line) in a 37ºC, 5% CO2 incubator for 24 hours and then dye loaded with ab112132 Green Dye for 30 minutes. The fluorescence intensity of Green Dye was measured with a flow cytometer using the FL1 channel.



This product has been referenced in:
  • Nance E  et al. Dendrimer-mediated delivery of N-acetyl cysteine to microglia in a mouse model of Rett syndrome. J Neuroinflammation 14:252 (2017). Read more (PubMed: 29258545) »
  • Veazey KJ  et al. Dose-dependent alcohol-induced alterations in chromatin structure persist beyond the window of exposure and correlate with fetal alcohol syndrome birth defects. Epigenetics Chromatin 8:39 (2015). Flow Cyt ; Mouse . Read more (PubMed: 26421061) »

See all 3 Publications for this product

Customer reviews and Q&As

The concentration of the dye used in the assay is toxic to cells, so these can not be further cultured.

Thank you for your enquiry and interest in our products.

MTT assay can be carried out on 96 well plates using either adherent or floating cells. You do not necessary need to remove (harvest) the cells by trypsinization. You can culture the ce...

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