Product nameAnti-Intrinsic Factor antibody [EPR13488(B)]
See all Intrinsic Factor primary antibodies
DescriptionRabbit monoclonal [EPR13488(B)] to Intrinsic Factor
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Human
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human Intrinsic Factor aa 400 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
Database link: P27352
- Human stomach lysate and Human stomach tissue
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab181045 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Perform heat mediated antigen retrieval, heat up to 98 degrees C, below boiling, and then let cool for 10-20 min, before commencing with IHC staining.
|WB||1/5000 - 1/10000. Predicted molecular weight: 45 kDa.|
RelevanceIntrinsic factor promotes absorption of the essential vitamin Cobalamin (Cbl) in the ileum by specific receptor mediated endocytosis. Defects in the gene GIF are the cause of hereditary intrinsic factor deficiency (IFD) also called congenital pernicious anemia. IFD is an autosomal recessive disorder characterized by megaloblastic anemia.
- Gastric Intrinsic Factor antibody
- Gastric intrinsic factor (vitamin B synthesis) antibody
- GIF antibody
Immunohistochemical analysis of paraffin-embedded Human stomach tissue labeling Intrinsic Factor using ab181045 at a 1/100 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Anti-Intrinsic Factor antibody [EPR13488(B)] (ab181045) at 1/5000 dilution + Human stomach lysate at 10 µg
Predicted band size: 45 kDa
ab181045 has not yet been referenced specifically in any publications.