Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13488(B)] to Intrinsic Factor - BSA and Azide free
- Suitable for: WB, IHC-P
- Reacts with: Human
Product nameAnti-Intrinsic Factor antibody [EPR13488(B)] - BSA and Azide free
See all Intrinsic Factor primary antibodies
DescriptionRabbit monoclonal [EPR13488(B)] to Intrinsic Factor - BSA and Azide free
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Synthetic peptide within Human Intrinsic Factor aa 400 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
Database link: P27352
- Human stomach lysate and Human stomach tissue
ab221500 is the carrier-free version of ab181045 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Ab221500 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C. Do Not Freeze.
Storage bufferpH: 7.2
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab221500 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration. Predicted molecular weight: 45 kDa.|
|IHC-P||Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Perform heat mediated antigen retrieval, heat up to 98 degrees C, below boiling, and then let cool for 10-20 min, before commencing with IHC staining.
RelevanceIntrinsic factor promotes absorption of the essential vitamin Cobalamin (Cbl) in the ileum by specific receptor mediated endocytosis. Defects in the gene GIF are the cause of hereditary intrinsic factor deficiency (IFD) also called congenital pernicious anemia. IFD is an autosomal recessive disorder characterized by megaloblastic anemia.
- Gastric Intrinsic Factor antibody
- Gastric intrinsic factor (vitamin B synthesis) antibody
- GIF antibody
This data was developed using ab181045, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human stomach tissue labeling Intrinsic Factor using ab181045 at a 1/100 dilution.Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab221500 has not yet been referenced specifically in any publications.