Recombinant
RabMAb

Recombinant Anti-Intrinsic Factor antibody [EPR13488(B)] - BSA and Azide free (ab221500)

Overview

  • Product name

    Anti-Intrinsic Factor antibody [EPR13488(B)] - BSA and Azide free
    See all Intrinsic Factor primary antibodies
  • Description

    Rabbit monoclonal [EPR13488(B)] to Intrinsic Factor - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Intrinsic Factor aa 400 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
    Database link: P27352

  • Positive control

    • Human stomach lysate and Human stomach tissue
  • General notes

    ab221500 is a PBS-only buffer format of ab181045. Please refer to ab181045 for recommended dilutions, protocols, and image data.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab221500 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 45 kDa.
IHC-P Use at an assay dependent concentration.

Perform heat mediated antigen retrieval, heat up to 98 degrees C, below boiling, and then let cool for 10-20 min, before commencing with IHC staining.

Target

  • Relevance

    Intrinsic factor promotes absorption of the essential vitamin Cobalamin (Cbl) in the ileum by specific receptor mediated endocytosis. Defects in the gene GIF are the cause of hereditary intrinsic factor deficiency (IFD) also called congenital pernicious anemia. IFD is an autosomal recessive disorder characterized by megaloblastic anemia.
  • Cellular localization

    Secreted
  • Database links

  • Alternative names

    • Gastric Intrinsic Factor antibody
    • Gastric intrinsic factor (vitamin B synthesis) antibody
    • GIF antibody
    • IF antibody
    • IFMH antibody
    • INF antibody
    • Intrinsic factor antibody
    • TCM3 antibody
    • TCN3 antibody
    see all

References

ab221500 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab221500.
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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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