Key features and details
- Rabbit polyclonal to INTS11
- Suitable for: WB, IP, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-INTS11 antibody
See all INTS11 primary antibodies
DescriptionRabbit polyclonal to INTS11
Tested applicationsSuitable for: WB, IP, IHC-Pmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Horse, Chimpanzee, Rhesus monkey, Gorilla, Opossum, Orangutan, Elephant
Synthetic peptide corresponding to Human INTS11.
Database link: NP_060341.2
- HeLa, 293T and NIH3T3 whole cell lysates.
This product was previously labelled as INT11
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab75276 was affinity purified using an epitope specific to INTS11 immobilized on solid support.
Our Abpromise guarantee covers the use of ab75276 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Detects a band of approximately 68 kDa (predicted molecular weight: 68 kDa).|
|IP||Use at 2-5 µg/mg of lysate.|
|IHC-P||1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionCatalytic component of the Integrator complex, a complex involved in the small nuclear RNAs (snRNA) U1 and U2 transcription and in their 3'-box-dependent processing. The Integrator complex is associated with the C-terminal domain (CTD) of RNA polymerase II largest subunit (POLR2A) and is recruited to the U1 and U2 snRNAs genes. Mediates the snRNAs 3' cleavage.
Sequence similaritiesBelongs to the metallo-beta-lactamase superfamily. RNA-metabolizing metallo-beta-lactamase-like family. INTS11 subfamily.
DomainThe HXHXDH motif is essential for the endoribonuclease activity of the CPSF complex.
Cellular localizationNucleus. Cytoplasm.
- Information by UniProt
- 2410006F12Rik antibody
- AU044035 antibody
- Cleavage and polyadenylation specific factor 3 like antibody
All lanes : Anti-INTS11 antibody (ab75276) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Lane 5 : NIH3T3 whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 68 kDa
Observed band size: 68 kDa
Exposure time: 3 minutes
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling INTS11 with ab75276 at 1/1000 (1µg/ml). Detection: DAB.
Detection of INTS11 by Western Blot of Immunprecipitate.
ab75278 at 1µg/ml staining INTS11 in HeLa whole cell lysate immunoprecipitated using ab75276 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 30 seconds.
ab75276 has been referenced in 3 publications.
- Xie M et al. The host Integrator complex acts in transcription-independent maturation of herpesvirus microRNA 3' ends. Genes Dev 29:1552-64 (2015). PubMed: 26220997
- O'Reilly D et al. Human snRNA genes use polyadenylation factors to promote efficient transcription termination. Nucleic Acids Res N/A:N/A (2013). WB ; Human . PubMed: 24097444
- Xie M et al. Mammalian 5'-capped microRNA precursors that generate a single microRNA. Cell 155:1568-80 (2013). PubMed: 24360278