Anti-INTS11 antibody (ab75276)
Key features and details
- Rabbit polyclonal to INTS11
- Suitable for: WB, IP, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-INTS11 antibody
See all INTS11 primary antibodies -
Description
Rabbit polyclonal to INTS11 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Horse, Chimpanzee, Rhesus monkey, Gorilla, Opossum, Orangutan, Elephant
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Immunogen
Synthetic peptide corresponding to Human INTS11.
Database link: NP_060341.2 -
Positive control
- HeLa, 293T and NIH3T3 whole cell lysates.
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General notes
This product was previously labelled as INT11
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab75276 was affinity purified using an epitope specific to INTS11 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab75276 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/2000 - 1/10000. Detects a band of approximately 68 kDa (predicted molecular weight: 68 kDa). | |
| IP | Use at 2-5 µg/mg of lysate. | |
| IHC-P | 1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Catalytic component of the Integrator complex, a complex involved in the small nuclear RNAs (snRNA) U1 and U2 transcription and in their 3'-box-dependent processing. The Integrator complex is associated with the C-terminal domain (CTD) of RNA polymerase II largest subunit (POLR2A) and is recruited to the U1 and U2 snRNAs genes. Mediates the snRNAs 3' cleavage. -
Sequence similarities
Belongs to the metallo-beta-lactamase superfamily. RNA-metabolizing metallo-beta-lactamase-like family. INTS11 subfamily. -
Domain
The HXHXDH motif is essential for the endoribonuclease activity of the CPSF complex. -
Cellular localization
Nucleus. Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 54973 Human
- Entrez Gene: 71957 Mouse
- Entrez Gene: 100171611 Orangutan
- Omim: 611354 Human
- SwissProt: Q5TA45 Human
- SwissProt: Q9CWS4 Mouse
- SwissProt: Q5NVE6 Orangutan
- Unigene: 6449 Human
see all -
Alternative names
- 2410006F12Rik antibody
- AU044035 antibody
- Cleavage and polyadenylation specific factor 3 like antibody
see all
Images
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Western blot - Anti-INTS11 antibody (ab75276)All lanes : Anti-INTS11 antibody (ab75276) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Lane 5 : NIH3T3 whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 68 kDa
Observed band size: 68 kDa
Exposure time: 3 minutes -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-INTS11 antibody (ab75276)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling INTS11 with ab75276 at 1/1000 (1µg/ml). Detection: DAB.
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Immunoprecipitation - Anti-INTS11 antibody (ab75276)Detection of INTS11 by Western Blot of Immunprecipitate.
ab75278 at 1µg/ml staining INTS11 in HeLa whole cell lysate immunoprecipitated using ab75276 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 30 seconds.
Protocols
Datasheets and documents
References (3)
ab75276 has been referenced in 3 publications.
- Xie M et al. The host Integrator complex acts in transcription-independent maturation of herpesvirus microRNA 3' ends. Genes Dev 29:1552-64 (2015). PubMed: 26220997
- O'Reilly D et al. Human snRNA genes use polyadenylation factors to promote efficient transcription termination. Nucleic Acids Res N/A:N/A (2013). WB ; Human . PubMed: 24097444
- Xie M et al. Mammalian 5'-capped microRNA precursors that generate a single microRNA. Cell 155:1568-80 (2013). PubMed: 24360278
