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Rabbit polyclonal to INTS5
Predicted to work with:
Horse, Chimpanzee, Ferret, Rhesus monkey, Gorilla, Chinese hamster
Synthetic peptide corresponding to a region between residues 1000 and 1050 of human INTS5 (NP_085131.1)
HeLa and NIH3T3 whole cell lysates.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS
Concentration information loading...
Immunogen affinity purified
ab74405 was affinity purified using an epitope specific to INTS5 immobilized on solid support.
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/2000 - 1/10000. Predicted molecular weight: 108 kDa.
Use at 2-5 µg/mg of lysate.
INTS5 is a subunit of the Integrator complex, a complex involved in the small nuclear RNAs (snRNA) U1 and U2 transcription and in their 3'-box-dependent processing. The Integrator complex is associated with the C-terminal domain (CTD) of RNA polymerase II largest subunit (POLR2A) and is recruited to the U1 and U2 snRNAs genes.
Nucleus. Membrane; Multi pass membrane protein.
integrator complex subunit 5 antibody
Western blot - Anti-INTS5 antibody (ab74405)
All lanes : Anti-INTS5 antibody (ab74405) at 0.4 µg/ml Lane 1 : HeLa whole cell lysate at 50 µg Lane 2 : HeLa whole cell lysate at 15 µg Lane 3 : HeLa whole cell lysate at 5 µg Lane 4 : NIH3T3 whole cell lysate at 50 µg Developed using the ECL technique. Predicted band size: 108 kDa Observed band size: 108 kDa Additional bands at: 75 kDa. We are unsure as to the identity of these extra bands. Exposure time: 10 seconds
Immunoprecipitation - Anti-INTS5 antibody (ab74405)
Detection of INTS5 by Western Blot of Immunprecipitate.
ab74405, at 1µg/ml, staining INTS5 in HeLa whole cell lysate immunoprecipitated using ab74405 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with exposure time of 30 seconds.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"