Key features and details
- Rabbit polyclonal to INTS5
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-INTS5 antibody
See all INTS5 primary antibodies
DescriptionRabbit polyclonal to INTS5
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Horse, Chimpanzee, Ferret, Rhesus monkey, Gorilla, Chinese hamster
Synthetic peptide corresponding to a region between residues 1000 and 1050 of human INTS5 (NP_085131.1)
- HeLa and NIH3T3 whole cell lysates.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab74405 was affinity purified using an epitope specific to INTS5 immobilized on solid support.
Our Abpromise guarantee covers the use of ab74405 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 108 kDa.|
|IP||Use at 2-5 µg/mg of lysate.|
RelevanceINTS5 is a subunit of the Integrator complex, a complex involved in the small nuclear RNAs (snRNA) U1 and U2 transcription and in their 3'-box-dependent processing. The Integrator complex is associated with the C-terminal domain (CTD) of RNA polymerase II largest subunit (POLR2A) and is recruited to the U1 and U2 snRNAs genes.
Cellular localizationNucleus. Membrane; Multi pass membrane protein.
- 1110055N21Rik antibody
- INT5 antibody
- integrator complex subunit 5 antibody
All lanes : Anti-INTS5 antibody (ab74405) at 0.4 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : NIH3T3 whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 108 kDa
Observed band size: 108 kDa
Additional bands at: 75 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 10 seconds
Detection of INTS5 by Western Blot of Immunprecipitate.
ab74405, at 1µg/ml, staining INTS5 in HeLa whole cell lysate immunoprecipitated using ab74405 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 30 seconds.
ab74405 has been referenced in 1 publication.
- Oegema R et al. Human mutations in integrator complex subunits link transcriptome integrity to brain development. PLoS Genet 13:e1006809 (2017). WB ; Human . PubMed: 28542170