Product nameAnti-INTS6 antibody
DescriptionRabbit polyclonal to INTS6
Tested applicationsSuitable for: WB, IP, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Rabbit, Horse, Cow, Pig, Chimpanzee, Rhesus monkey, Gorilla, Opossum, Orangutan, Elephant
Synthetic peptide corresponding to Human INTS6.
Previously labelled as DDX26.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
- Epigenetics and Nuclear Signaling
- Polymerase associated factors
- Pol II Transcription
Our Abpromise guarantee covers the use of ab86369 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 100 kDa.|
|IP||Use a concentration of 2 - 5 µg/ml.|
|IHC-P||1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionComponent of the Integrator complex, a complex involved in the small nuclear RNAs (snRNA) U1 and U2 transcription and in their 3'-box-dependent processing. The Integrator complex is associated with the C-terminal domain (CTD) of RNA polymerase II largest subunit (POLR2A) and is recruited to the U1 and U2 snRNAs genes. May have a tumor suppressor role; an ectopic expression suppressing tumor cell growth.
Tissue specificityWidely expressed. Expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.
Sequence similaritiesBelongs to the Integrator subunit 6 family.
Contains 1 VWFA domain.
- Information by UniProt
- DBI 1 antibody
- DBI-1 antibody
- DBI1 antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human thyroid carcinoma tissue labelling INTS6 with ab86369 at 1/200 (1µg/ml). Detection: DAB.
All lanes : Anti-INTS6 antibody (ab86369) at 0.04 µg/ml
Lane 1 : Whole cell lysate from HeLa cells at 50 µg
Lane 2 : Whole cell lysate from HeLa cells at 15 µg
Lane 3 : Whole cell lysate from HeLa cells at 5 µg
Predicted band size: 100 kDa
Observed band size: 117 kDa why is the actual band size different from the predicted?
ab86369 has not yet been referenced specifically in any publications.