Anti-INTS9 antibody (ab234700)
Key features and details
- Rabbit polyclonal to INTS9
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-INTS9 antibody -
Description
Rabbit polyclonal to INTS9 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Cow, Cynomolgus monkey -
Immunogen
Recombinant fragment corresponding to Human INTS9 aa 350 to the C-terminus.
Database link: Q9NV88 -
Positive control
- WB: Jurkat, HeLa and MCF7 whole cell lysates. IHC-P: Human prostate cancer and ovarian cancer tissue. ICC/IF: HeLa cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.03% Proclin 300
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
Purity >95%. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab234700 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/20 - 1/200.
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WB |
1/2000 - 1/5000. Predicted molecular weight: 74, 71 kDa.
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ICC/IF |
1/50 - 1/200.
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Notes |
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IHC-P
1/20 - 1/200. |
WB
1/2000 - 1/5000. Predicted molecular weight: 74, 71 kDa. |
ICC/IF
1/50 - 1/200. |
Target
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Function
Component of the Integrator complex, a complex involved in the small nuclear RNAs (snRNA) U1 and U2 transcription and in their 3'-box-dependent processing. The Integrator complex is associated with the C-terminal domain (CTD) of RNA polymerase II largest subunit (POLR2A) and is recruited to the U1 and U2 snRNAs genes. -
Sequence similarities
Belongs to the metallo-beta-lactamase superfamily. RNA-metabolizing metallo-beta-lactamase-like family. INTS9 subfamily. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 533964 Cow
- Entrez Gene: 101867367 Cynomolgus monkey
- Entrez Gene: 55756 Human
- Omim: 611352 Human
- SwissProt: Q2KJA6 Cow
- SwissProt: Q4R5Z4 Cynomolgus monkey
- SwissProt: Q9NV88 Human
- Unigene: 162397 Human
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Alternative names
- CPSF antibody
- CPSF2 antibody
- CPSF2L antibody
see all
Images
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All lanes : Anti-INTS9 antibody (ab234700) at 1/2000 dilution
Lane 1 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 3 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate
Secondary
All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 74, 71 kDa -
Paraffin-embedded human prostate cancer tissue stained for INTS9 using ab234700 at 1/100 dilution in immunohistochemical analysis.
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Paraffin-embedded human ovarian cancer tissue stained for INTS9 using ab234700 at 1/100 dilution in immunohistochemical analysis.
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HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for INTS9 (green) using ab234700 at 1/100 dilution in ICC/IF, followed by Alexa Fluor 488® congugated Goat Anti-Rabbit IgG (H+L).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab234700 has not yet been referenced specifically in any publications.