Invertase Activity Assay Kit (Colorimetric) (ab197005)

Overview

  • Product name

    Invertase Activity Assay Kit (Colorimetric)
  • Detection method

    Colorimetric
  • Sample type

    Saliva, Other biological fluids, Purified protein
  • Assay type

    Enzyme activity
  • Sensitivity

    20 µU
  • Species reactivity

    Reacts with: Other species, Mammals
  • Product overview

    Invertase Activity Assay Kit (Colorimetric) (197005)  provides a simple, sensitive and high-throughput adaptable assay that detects relevant concentrations of Invertase in biological samples or purified/crude enzyme preparations. The assay is based on the enzymatic detection of glucose, one of the hydrolysis products. Detection limit: 20 µU.

  • Notes

    Invertase (EC 3.2.1.26), also known as sucrase or β -fructofuranosidase, catalyzes the hydrolysis of sucrose (table sugar) by cleaving its glycosidic bond and forming one molecule each of glucose and fructose. Invertase is widely expressed among plants and microorganisms including yeast and bacteria. It is extensively utilized in industrial processes, including food, ethanol production, cosmetics, drugs, paper and bioelectronics electrodes.

  • Platform

    Microplate reader

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components Identifier 100 tests
    Invertase Hydrolysis Buffer 1 x 25ml
    Glucose Standard (100 mM) 1 x 100µl
    Invertase Assay Buffer 1 x 25ml
    Invertase Enzyme Mix 1 vial
    Invertase Stop Solution 1 x 1ml
    Invertase Substrate 1 x 1.2ml
    OxiRed Probe Red 1 x 0.2ml
    Positive Control 1 vial

Images

  • Detection of bacterial Invertase activity in human saliva. Samples collected: 1-Fasting; 2-Low calorie meal and 3: High Sucrose meal. Undiluted samples (1 and 2 = 25 μl and 3 = 10 μl) were incubated for 20 min with Invertase substrate. Assay was performed following the kit protocol.
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Protocols

References

ab197005 has not yet been referenced specifically in any publications.

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