Anti-Involucrin antibody (ab53112)
Key features and details
- Rabbit polyclonal to Involucrin
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Involucrin antibody
See all Involucrin primary antibodies -
Description
Rabbit polyclonal to Involucrin -
Host species
Rabbit -
Specificity
ab53112 detects endogenous levels of total Involucrin protein. -
Tested Applications & Species
Application Species IHC-P HumanWB Human -
Immunogen
A synthetic peptide derived from human Involucrin.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 0.87% Sodium chloride, PBS
Without Mg+2 and Ca+2 -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab53112 was affinity purified from rabbit antiserum by affinity chromatography using epitope-specific immunogen. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab53112 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
Application | Species |
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IHC-P |
Human
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WB |
Human
|
Application | Abreviews | Notes |
---|---|---|
WB |
1/300 - 1/1000. Detects a band of approximately 68 kDa (predicted molecular weight: 68 kDa).
|
|
IHC-P | (5) |
Use at an assay dependent concentration.
|
Notes |
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WB
1/300 - 1/1000. Detects a band of approximately 68 kDa (predicted molecular weight: 68 kDa). |
IHC-P
Use at an assay dependent concentration. |
Target
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Function
Part of the insoluble cornified cell envelope (CE) of stratified squamous epithelia. -
Tissue specificity
Keratinocytes of epidermis and other stratified squamous epithelia. -
Sequence similarities
Belongs to the involucrin family. -
Post-translational
modificationsSubstrate of transglutaminase. Some glutamines and lysines are cross-linked to other involucrin molecules, to other proteins such as keratin, desmoplakin, periplakin and envoplakin, and to lipids like omega-hydroxyceramide. -
Cellular localization
Cytoplasm. Constituent of the scaffolding of the cornified envelope. - Information by UniProt
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Database links
- Entrez Gene: 3713 Human
- Omim: 147360 Human
- SwissProt: P07476 Human
- Unigene: 516439 Human
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Alternative names
- INVO_HUMAN antibody
- Involucrin antibody
- IVL antibody
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Involucrin antibody (ab53112)This image is courtesy of an Abreview submitted by Carl Hobbs
ab53112 staining human skin sections by IHC-P. The tissue was fixed with formaldehyde and a heat mediated antigen retrival step was performed with citric acid pH 6. Blocking of the sample was done with 1% BSA for 10 minutes at 21°C, followed by staining with ab53112 at 1/150 in TBS/BSA/azide for 16h at 21°C. A biotinylated goat anti-rabbit polyclonal antibody at 1/200 was used as the secondary antibody.
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All lanes : Anti-Involucrin antibody (ab53112) at 1/300 dilution
Lane 1 : HuvEc cell extract
Lane 2 : HuvEc cell extract with immunising peptide
Predicted band size: 68 kDa
Observed band size: 68 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Involucrin antibody (ab53112)ab53112 at 1/50 dilution staining Involucrin in human breast carcinoma by Immunohistochemistry, Paraffin embedded tissue, in the absence and presence of the immunising peptide.
Protocols
References (16)
ab53112 has been referenced in 16 publications.
- Arasteh S et al. Efficient Wound Healing Using a Synthetic Nanofibrous Bilayer Skin Substitute in Murine Model. J Surg Res 245:31-44 (2020). PubMed: 31400575
- Fortunel NO et al. Iterative Three-Dimensional Epidermis Bioengineering and Xenografting to Assess Long-Term Regenerative Potential in Human Keratinocyte Precursor Cells. Methods Mol Biol N/A:N/A (2019). PubMed: 31309517
- Meunier M et al. From stem cells protection to skin microbiota balance: Orobanche rapum extract, a new natural strategy. J Cosmet Dermatol 18:1140-1154 (2019). PubMed: 30485658
- Ya C et al. Substrate softness promotes terminal differentiation of human keratinocytes without altering their ability to proliferate back into a rigid environment. Arch Dermatol Res N/A:N/A (2019). PubMed: 31392392
- Horvath S et al. Rapamycin retards epigenetic ageing of keratinocytes independently of its effects on replicative senescence, proliferation and differentiation. Aging (Albany NY) 11:3238-3249 (2019). PubMed: 31136303