RecombinantRabMAb

Recombinant Anti-Involucrin antibody [EPR13054] (ab181980)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Involucrin antibody [EPR13054] (ab181980)
  • Western blot - Anti-Involucrin antibody [EPR13054] (ab181980)
  • Western blot - Anti-Involucrin antibody [EPR13054] (ab181980)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Involucrin antibody [EPR13054] (ab181980)
  • Flow Cytometry (Intracellular) - Anti-Involucrin antibody [EPR13054] (ab181980)
  • Anti-Involucrin antibody [EPR13054] (ab181980)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13054] to Involucrin
  • Suitable for: Flow Cyt (Intra), IHC-P, WB
  • Reacts with: Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

Overview

  • Product name

    Anti-Involucrin antibody [EPR13054]
    See all Involucrin primary antibodies

  • Description

    Rabbit monoclonal [EPR13054] to Involucrin

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt (Intra), IHC-P, WBmore details

  • Species reactivity

    Reacts with: Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • SW480 and Human fetal skin lysates; Human skin and tonsil tissues; HUVEC cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR13054

  • Isotype

    IgG

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab181980 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt (Intra)
1/10.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
IHC-P
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB
1/10000 - 1/50000. Detects a band of approximately 120 kDa (predicted molecular weight: 68 kDa).
Notes
Flow Cyt (Intra)
1/10.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
IHC-P
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB
1/10000 - 1/50000. Detects a band of approximately 120 kDa (predicted molecular weight: 68 kDa).

Target

  • Function

    Part of the insoluble cornified cell envelope (CE) of stratified squamous epithelia.

  • Tissue specificity

    Keratinocytes of epidermis and other stratified squamous epithelia.

  • Sequence similarities

    Belongs to the involucrin family.

  • Post-translational
    modifications

    Substrate of transglutaminase. Some glutamines and lysines are cross-linked to other involucrin molecules, to other proteins such as keratin, desmoplakin, periplakin and envoplakin, and to lipids like omega-hydroxyceramide.

  • Cellular localization

    Cytoplasm. Constituent of the scaffolding of the cornified envelope.
  • Information by UniProt

  • Database links

    • Alternative names

      • INVO_HUMAN antibody
      • Involucrin antibody
      • IVL antibody

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Involucrin antibody [EPR13054] (ab181980)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Involucrin antibody [EPR13054] (ab181980)

      Immunohistochemical analysis of paraffin-embedded Human skin tissue labeling Involucrin with ab181980 at 1/100 dilution, followed by prediluted ImmunoHistoprobe (Ready to use) HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

      Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

    • Western blot - Anti-Involucrin antibody [EPR13054] (ab181980)
      Western blot - Anti-Involucrin antibody [EPR13054] (ab181980)
      All lanes : Anti-Involucrin antibody [EPR13054] (ab181980) at 1/500000 dilution

      Lane 1 : HaCaT (Human keratinocyte cell line) whole cell lysate
      Lane 2 : A431 (Human epidermoid carcinoma cell line) whole cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

      Predicted band size: 68 kDa
      Observed band size: 120 kDa why is the actual band size different from the predicted?


      Exposure time: 1 minute


      Diluting and blocking buffer: 5% NFDM/TBST

    • Western blot - Anti-Involucrin antibody [EPR13054] (ab181980)
      Western blot - Anti-Involucrin antibody [EPR13054] (ab181980)
      All lanes :

      Lane 1 : Human fetal skin tissue
      Lane 2 : Rat skin tissue

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

      Predicted band size: 68 kDa
      Observed band size: 120 kDa why is the actual band size different from the predicted?


      Exposure time: 1 minute


      Diluting and blocking buffer: 5% NFDM/TBST

       

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Involucrin antibody [EPR13054] (ab181980)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Involucrin antibody [EPR13054] (ab181980)

      Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling Involucrin with ab181980 at 1/100 dilution, followed by prediluted ImmunoHistoprobe (Ready to use) HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

      Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

    • Flow Cytometry (Intracellular) - Anti-Involucrin antibody [EPR13054] (ab181980)
      Flow Cytometry (Intracellular) - Anti-Involucrin antibody [EPR13054] (ab181980)

      Intracellular flow cytometric analysis of 2% paraformaldehyde-fixed HUVEC cells labeling Involucrin with ab181980 at 1/10 dilution (red) compared to a Rabbit monoclonal IgG Isotype control (green), followed by Goat anti rabbit IgG (FITC) secondary antibody at 1/150 dilution. 

       

       

    • Anti-Involucrin antibody [EPR13054] (ab181980)
      Anti-Involucrin antibody [EPR13054] (ab181980)

    References (0)

    Publishing research using ab181980? Please let us know so that we can cite the reference in this datasheet.

    ab181980 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    Immunohistochemistry (Frozen sections) abreview for Anti-Involucrin antibody [EPR13054]

     Excellent
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Human Tissue sections (skin)
    Permeabilization
    No
    Specification
    skin
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 25°C
    Fixative
    Acetone
    Read More

    Mrs. Laurène ROMANO

    Verified customer

    Submitted May 26 2020

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