Recombinant
RabMAb

Anti-Involucrin antibody [EPR13054] (ab181980)

Overview

  • Product name
    Anti-Involucrin antibody [EPR13054]
    See all Involucrin primary antibodies
  • Description
    Rabbit monoclonal [EPR13054] to Involucrin
  • Host species
    Rabbit
  • Tested applications
    Suitable for: Flow Cyt, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Rat, Human
  • Immunogen

    Synthetic peptide within Human Involucrin aa 150-250. The exact sequence is proprietary.
    Database link: P07476

  • Positive control
    • SW480 and Human fetal skin lysates; Human skin and tonsil tissues; HUVEC cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab181980 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/10.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

IHC-P 1/100.
WB 1/10000 - 1/50000. Detects a band of approximately 120 kDa (predicted molecular weight: 68 kDa).

Target

  • Function
    Part of the insoluble cornified cell envelope (CE) of stratified squamous epithelia.
  • Tissue specificity
    Keratinocytes of epidermis and other stratified squamous epithelia.
  • Sequence similarities
    Belongs to the involucrin family.
  • Post-translational
    modifications
    Substrate of transglutaminase. Some glutamines and lysines are cross-linked to other involucrin molecules, to other proteins such as keratin, desmoplakin, periplakin and envoplakin, and to lipids like omega-hydroxyceramide.
  • Cellular localization
    Cytoplasm. Constituent of the scaffolding of the cornified envelope.
  • Information by UniProt
  • Database links
  • Alternative names
    • INVO_HUMAN antibody
    • Involucrin antibody
    • IVL antibody

Images

  • All lanes : Anti-Involucrin antibody [EPR13054] (ab181980) at 1/500000 dilution

    Lane 1 : HaCaT (Human keratinocyte cell line) whole cell lysate
    Lane 2 : A431 (Human epidermoid carcinoma cell line) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 68 kDa
    Observed band size: 120 kDa (why is the actual band size different from the predicted?)


    Exposure time: 1 minute


    Diluting and blocking buffer: 5% NFDM/TBST

  • All lanes :

    Lane 1 : Human fetal skin tissue
    Lane 2 : Rat skin tissue

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 68 kDa
    Observed band size: 120 kDa (why is the actual band size different from the predicted?)


    Exposure time: 1 minute


    Diluting and blocking buffer: 5% NFDM/TBST

     

  • Immunohistochemical analysis of paraffin-embedded Human skin tissue labeling Involucrin with ab181980 at 1/100 dilution, followed by prediluted ImmunoHistoprobe (Ready to use) HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

  • Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling Involucrin with ab181980 at 1/100 dilution, followed by prediluted ImmunoHistoprobe (Ready to use) HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

  • Flow cytometric analysis of 2% paraformaldehyde-fixed HUVEC cells labeling Involucrin with ab181980 at 1/10 dilution (red) compared to a Rabbit monoclonal IgG Isotype control (green), followed by Goat anti rabbit IgG (FITC) secondary antibody at 1/150 dilution.

References

ab181980 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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