Recombinant
RabMAb

Recombinant Anti-Involucrin antibody [EPR13054] (ab181980)

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Overview

  • Product name

    Anti-Involucrin antibody [EPR13054]
    See all Involucrin primary antibodies

  • Description

    Rabbit monoclonal [EPR13054] to Involucrin

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt, IHC-P, WBmore details

  • Species reactivity

    Reacts with: Rat, Human

  • Immunogen

    Synthetic peptide within Human Involucrin aa 150-250. The exact sequence is proprietary.
    Database link: P07476

  • Positive control

    • SW480 and Human fetal skin lysates; Human skin and tonsil tissues; HUVEC cells.

  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab181980 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/10.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 
IHC-P 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB 1/10000 - 1/50000. Detects a band of approximately 120 kDa (predicted molecular weight: 68 kDa).

Target

  • Function

    Part of the insoluble cornified cell envelope (CE) of stratified squamous epithelia.

  • Tissue specificity

    Keratinocytes of epidermis and other stratified squamous epithelia.

  • Sequence similarities

    Belongs to the involucrin family.

  • Post-translational
    modifications

    Substrate of transglutaminase. Some glutamines and lysines are cross-linked to other involucrin molecules, to other proteins such as keratin, desmoplakin, periplakin and envoplakin, and to lipids like omega-hydroxyceramide.

  • Cellular localization

    Cytoplasm. Constituent of the scaffolding of the cornified envelope.
  • Information by UniProt

  • Database links

    • Alternative names

      • INVO_HUMAN antibody
      • Involucrin antibody
      • IVL antibody

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Involucrin antibody [EPR13054] (ab181980)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Involucrin antibody [EPR13054] (ab181980)

      Immunohistochemical analysis of paraffin-embedded Human skin tissue labeling Involucrin with ab181980 at 1/100 dilution, followed by prediluted ImmunoHistoprobe (Ready to use) HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

      Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

    • Western blot - Anti-Involucrin antibody [EPR13054] (ab181980)
      Western blot - Anti-Involucrin antibody [EPR13054] (ab181980)
      All lanes : Anti-Involucrin antibody [EPR13054] (ab181980) at 1/500000 dilution

      Lane 1 : HaCaT (Human keratinocyte cell line) whole cell lysate
      Lane 2 : A431 (Human epidermoid carcinoma cell line) whole cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

      Predicted band size: 68 kDa
      Observed band size: 120 kDa
      why is the actual band size different from the predicted?


      Exposure time: 1 minute


      Diluting and blocking buffer: 5% NFDM/TBST

    • Western blot - Anti-Involucrin antibody [EPR13054] (ab181980)
      Western blot - Anti-Involucrin antibody [EPR13054] (ab181980)
      All lanes :

      Lane 1 : Human fetal skin tissue
      Lane 2 : Rat skin tissue

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

      Predicted band size: 68 kDa
      Observed band size: 120 kDa why is the actual band size different from the predicted?


      Exposure time: 1 minute


      Diluting and blocking buffer: 5% NFDM/TBST

       

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Involucrin antibody [EPR13054] (ab181980)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Involucrin antibody [EPR13054] (ab181980)

      Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling Involucrin with ab181980 at 1/100 dilution, followed by prediluted ImmunoHistoprobe (Ready to use) HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

      Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

    • Flow Cytometry - Anti-Involucrin antibody [EPR13054] (ab181980)
      Flow Cytometry - Anti-Involucrin antibody [EPR13054] (ab181980)

      Flow cytometric analysis of 2% paraformaldehyde-fixed HUVEC cells labeling Involucrin with ab181980 at 1/10 dilution (red) compared to a Rabbit monoclonal IgG Isotype control (green), followed by Goat anti rabbit IgG (FITC) secondary antibody at 1/150 dilution.

    References

    ab181980 has not yet been referenced specifically in any publications.

    Publishing research using ab181980? Please let us know so that we can cite the reference in this datasheet.

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