
Recombinant Anti-Involucrin antibody [EPR13054] (ab181980)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-Involucrin antibody [EPR13054]
See all Involucrin primary antibodies -
Description
Rabbit monoclonal [EPR13054] to Involucrin -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt, IHC-P, WBmore details -
Species reactivity
Reacts with: Rat, Human -
Immunogen
Synthetic peptide within Human Involucrin aa 150-250. The exact sequence is proprietary.
Database link: P07476 -
Positive control
- SW480 and Human fetal skin lysates; Human skin and tonsil tissues; HUVEC cells.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR13054 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab181980 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt | 1/10. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
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IHC-P | 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. | |
WB | 1/10000 - 1/50000. Detects a band of approximately 120 kDa (predicted molecular weight: 68 kDa). |
Target
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Function
Part of the insoluble cornified cell envelope (CE) of stratified squamous epithelia. -
Tissue specificity
Keratinocytes of epidermis and other stratified squamous epithelia. -
Sequence similarities
Belongs to the involucrin family. -
Post-translational
modificationsSubstrate of transglutaminase. Some glutamines and lysines are cross-linked to other involucrin molecules, to other proteins such as keratin, desmoplakin, periplakin and envoplakin, and to lipids like omega-hydroxyceramide. -
Cellular localization
Cytoplasm. Constituent of the scaffolding of the cornified envelope. - Information by UniProt
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Database links
- Entrez Gene: 3713 Human
- Entrez Gene: 60583 Rat
- Omim: 147360 Human
- SwissProt: P07476 Human
- SwissProt: P48998 Rat
- Unigene: 516439 Human
- Unigene: 142591 Rat
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Alternative names
- INVO_HUMAN antibody
- Involucrin antibody
- IVL antibody
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Involucrin antibody [EPR13054] (ab181980)
Immunohistochemical analysis of paraffin-embedded Human skin tissue labeling Involucrin with ab181980 at 1/100 dilution, followed by prediluted ImmunoHistoprobe (Ready to use) HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
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All lanes : Anti-Involucrin antibody [EPR13054] (ab181980) at 1/500000 dilution
Lane 1 : HaCaT (Human keratinocyte cell line) whole cell lysate
Lane 2 : A431 (Human epidermoid carcinoma cell line) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 68 kDa
Observed band size: 120 kDa why is the actual band size different from the predicted?
Exposure time: 1 minuteDiluting and blocking buffer: 5% NFDM/TBST
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All lanes :
Lane 1 : Human fetal skin tissue
Lane 2 : Rat skin tissue
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 68 kDa
Observed band size: 120 kDa why is the actual band size different from the predicted?
Exposure time: 1 minuteDiluting and blocking buffer: 5% NFDM/TBST
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Involucrin antibody [EPR13054] (ab181980)
Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling Involucrin with ab181980 at 1/100 dilution, followed by prediluted ImmunoHistoprobe (Ready to use) HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
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Flow cytometric analysis of 2% paraformaldehyde-fixed HUVEC cells labeling Involucrin with ab181980 at 1/10 dilution (red) compared to a Rabbit monoclonal IgG Isotype control (green), followed by Goat anti rabbit IgG (FITC) secondary antibody at 1/150 dilution.
Datasheets and documents
References
ab181980 has not yet been referenced specifically in any publications.