RecombinantRabMAb

Recombinant Anti-Involucrin antibody [EPR13054] (ab181980)

Reviews (1) Submit a question

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13054] to Involucrin
  • Suitable for: Flow Cyt, IHC-P, WB
  • Reacts with: Rat, Human

Overview

  • Product name

    Anti-Involucrin antibody [EPR13054]
    See all Involucrin primary antibodies

  • Description

    Rabbit monoclonal [EPR13054] to Involucrin

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt, IHC-P, WBmore details

  • Species reactivity

    Reacts with: Rat, Human

  • Immunogen

    Synthetic peptide within Human Involucrin aa 150-250. The exact sequence is proprietary.
    Database link: P07476

  • Positive control

    • SW480 and Human fetal skin lysates; Human skin and tonsil tissues; HUVEC cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR13054

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab181980 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/10.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 
IHC-P 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB 1/10000 - 1/50000. Detects a band of approximately 120 kDa (predicted molecular weight: 68 kDa).

Target

  • Function

    Part of the insoluble cornified cell envelope (CE) of stratified squamous epithelia.

  • Tissue specificity

    Keratinocytes of epidermis and other stratified squamous epithelia.

  • Sequence similarities

    Belongs to the involucrin family.

  • Post-translational
    modifications

    Substrate of transglutaminase. Some glutamines and lysines are cross-linked to other involucrin molecules, to other proteins such as keratin, desmoplakin, periplakin and envoplakin, and to lipids like omega-hydroxyceramide.

  • Cellular localization

    Cytoplasm. Constituent of the scaffolding of the cornified envelope.
  • Information by UniProt

  • Database links

    • Alternative names

      • INVO_HUMAN antibody
      • Involucrin antibody
      • IVL antibody

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Involucrin antibody [EPR13054] (ab181980)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Involucrin antibody [EPR13054] (ab181980)

      Immunohistochemical analysis of paraffin-embedded Human skin tissue labeling Involucrin with ab181980 at 1/100 dilution, followed by prediluted ImmunoHistoprobe (Ready to use) HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

      Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

    • Western blot - Anti-Involucrin antibody [EPR13054] (ab181980)
      Western blot - Anti-Involucrin antibody [EPR13054] (ab181980)
      All lanes : Anti-Involucrin antibody [EPR13054] (ab181980) at 1/500000 dilution

      Lane 1 : HaCaT (Human keratinocyte cell line) whole cell lysate
      Lane 2 : A431 (Human epidermoid carcinoma cell line) whole cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

      Predicted band size: 68 kDa
      Observed band size: 120 kDa
      why is the actual band size different from the predicted?


      Exposure time: 1 minute


      Diluting and blocking buffer: 5% NFDM/TBST

    • Western blot - Anti-Involucrin antibody [EPR13054] (ab181980)
      Western blot - Anti-Involucrin antibody [EPR13054] (ab181980)
      All lanes :

      Lane 1 : Human fetal skin tissue
      Lane 2 : Rat skin tissue

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

      Predicted band size: 68 kDa
      Observed band size: 120 kDa why is the actual band size different from the predicted?


      Exposure time: 1 minute


      Diluting and blocking buffer: 5% NFDM/TBST

       

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Involucrin antibody [EPR13054] (ab181980)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Involucrin antibody [EPR13054] (ab181980)

      Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling Involucrin with ab181980 at 1/100 dilution, followed by prediluted ImmunoHistoprobe (Ready to use) HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

      Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

    • Flow Cytometry - Anti-Involucrin antibody [EPR13054] (ab181980)
      Flow Cytometry - Anti-Involucrin antibody [EPR13054] (ab181980)

      Flow cytometric analysis of 2% paraformaldehyde-fixed HUVEC cells labeling Involucrin with ab181980 at 1/10 dilution (red) compared to a Rabbit monoclonal IgG Isotype control (green), followed by Goat anti rabbit IgG (FITC) secondary antibody at 1/150 dilution.

    • Anti-Involucrin antibody [EPR13054] (ab181980)
      Anti-Involucrin antibody [EPR13054] (ab181980)

    References (0)

    Publishing research using ab181980? Please let us know so that we can cite the reference in this datasheet.

    ab181980 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Immunohistochemistry (Frozen sections) abreview for Anti-Involucrin antibody [EPR13054]

     Excellent
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Human Tissue sections (skin)
    Permeabilization
    No
    Specification
    skin
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 25°C
    Fixative
    Acetone
    Read More

    Mrs. Laurène ROMANO

    Verified customer

    Submitted May 26 2020

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com