Recombinant
RabMAb

Recombinant Anti-Involucrin antibody [EPR13060(N)] - C-terminal (ab184969)

Overview

  • Product name

    Anti-Involucrin antibody [EPR13060(N)] - C-terminal
    See all Involucrin primary antibodies
  • Description

    Rabbit monoclonal [EPR13060(N)] to Involucrin - C-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, Flow Cytmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human Involucrin aa 500 to the C-terminus. The exact sequence is proprietary.
    Database link: P07476

  • Positive control

    • Human fetal skin and HT-1080 cell lysates; HACAT cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR13060(N)
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab184969 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Detects a band of approximately 140 kDa (predicted molecular weight: 68 kDa).
Flow Cyt 1/40.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

Target

  • Function

    Part of the insoluble cornified cell envelope (CE) of stratified squamous epithelia.
  • Tissue specificity

    Keratinocytes of epidermis and other stratified squamous epithelia.
  • Sequence similarities

    Belongs to the involucrin family.
  • Post-translational
    modifications

    Substrate of transglutaminase. Some glutamines and lysines are cross-linked to other involucrin molecules, to other proteins such as keratin, desmoplakin, periplakin and envoplakin, and to lipids like omega-hydroxyceramide.
  • Cellular localization

    Cytoplasm. Constituent of the scaffolding of the cornified envelope.
  • Information by UniProt
  • Database links

  • Alternative names

    • INVO_HUMAN antibody
    • Involucrin antibody
    • IVL antibody

Images

  • Anti-Involucrin antibody [EPR13060(N)] - C-terminal (ab184969) + Human fetal skin lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 68 kDa

  • Flow cytometric analysis of Involucrin expression in paraformaldehyde-fixed HACAT cells using ab184969 at 1/40 dilution (red) and a rabbit IgG as negative control (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.

  • Anti-Involucrin antibody [EPR13060(N)] - C-terminal (ab184969) at 1/50000 dilution + HT-1080 cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 68 kDa

References

ab184969 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab184969.
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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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