Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [IVRN/2113R] to Involucrin
- Suitable for: IHC-P
- Reacts with: Human
Product nameAnti-Involucrin antibody [IVRN/2113R]
See all Involucrin primary antibodies
DescriptionRabbit monoclonal [IVRN/2113R] to Involucrin
Tested applicationsSuitable for: IHC-Pmore details
Species reactivityReacts with: Human
Does not react with: Mouse
Recombinant full length protein corresponding to Human Involucrin aa 1-585.
MSQQHTLPVTLSPALSQELLKTVPPPVNTHQEQMKQPTPLPPPCQKVPVE LPVEVPSKQEEKHMTAVKGLPEQECEQQQKEPQEQELQQQHWEQHEEYQK AENPEQQLKQEKTQRDQQLNKQLEEEKKLLDQQLDQELVKRDEQLGMKKE QLLELPEQQEGHLKHLEQQEGQLKHPEQQEGQLELPEQQEGQLELPEQQE GQLELPEQQEGQLELPEQQEGQLELPEQQEGQLELPQQQEGQLELSEQQE GQLELSEQQEGQLKHLEHQEGQLEVPEEQMGQLKYLEQQEGQLKHLDQQE KQPELPEQQMGQLKHLEQQEGQPKHLEQQEGQLEQLEEQEGQLKHLEQQE GQLEHLEHQEGQLGLPEQQVLQLKQLEKQQGQPKHLEEEEGQLKHLVQQE GQLKHLVQQEGQLEQQERQVEHLEQQVGQLKHLEEQEGQLKHLEQQQGQL EVPEQQVGQPKNLEQEEKQLELPEQQEGQVKHLEKQEAQLELPEQQVGQP KHLEQQEKHLEHPEQQDGQLKHLEQQEGQLKDLEQQKGQLEQPVFAPAPG QVQDIQPALPTKGEVLLPVEHQQQKQEVQWPPKHK
Database link: P07476
- IHC-P: Human tonsil tissue.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.05% Sodium azide
Constituents: PBS, 0.05% BSA
Concentration information loading...
PurityProtein A/G purified
Purification notesPurified from bioreactor concentrate by Protein A/G.
Our Abpromise guarantee covers the use of ab234403 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 0.1 - 0.2 µg/ml.
Staining of formalin-fixed tissues is enhanced by digestion with trypsin or Protease XXV at 1 mg/ml PBS for 5 minutes at 37°C. (Note than enzyme digestion is better than citrate buffer based epitope unmasking). Incubate with primary antibody for 30 minutes at room temperature.
FunctionPart of the insoluble cornified cell envelope (CE) of stratified squamous epithelia.
Tissue specificityKeratinocytes of epidermis and other stratified squamous epithelia.
Sequence similaritiesBelongs to the involucrin family.
modificationsSubstrate of transglutaminase. Some glutamines and lysines are cross-linked to other involucrin molecules, to other proteins such as keratin, desmoplakin, periplakin and envoplakin, and to lipids like omega-hydroxyceramide.
Cellular localizationCytoplasm. Constituent of the scaffolding of the cornified envelope.
- Information by UniProt
- INVO_HUMAN antibody
- Involucrin antibody
- IVL antibody
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab234403 has not yet been referenced specifically in any publications.