Recombinant Anti-Involucrin antibody [IVRN/2113R] - BSA and Azide free (ab236273)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [IVRN/2113R] to Involucrin - BSA and Azide free
- Suitable for: IHC-P
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Involucrin antibody [IVRN/2113R] - BSA and Azide free
See all Involucrin primary antibodies -
Description
Rabbit monoclonal [IVRN/2113R] to Involucrin - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Human
Does not react with: Mouse -
Immunogen
Recombinant full length protein corresponding to Human Involucrin aa 1 to the C-terminus.
Database link: P07476 -
Positive control
- IHC-P: Human tonsil tissue.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A/G purified -
Purification notes
Purified from bioreactor concentrate by Protein A/G. -
Clonality
Monoclonal -
Clone number
IVRN/2113R -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab236273 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
Use a concentration of 0.1 - 0.2 µg/ml.
Staining of formalin-fixed tissues is enhanced by digestion with trypsin or Protease XXV at 1 mg/ml PBS for 5 minutes at 37°C. (Note than enzyme digestion is better than citrate buffer based epitope unmasking). Incubate with primary antibody for 30 minutes at room temperature. |
Notes |
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IHC-P
Use a concentration of 0.1 - 0.2 µg/ml. Staining of formalin-fixed tissues is enhanced by digestion with trypsin or Protease XXV at 1 mg/ml PBS for 5 minutes at 37°C. (Note than enzyme digestion is better than citrate buffer based epitope unmasking). Incubate with primary antibody for 30 minutes at room temperature. |
Target
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Function
Part of the insoluble cornified cell envelope (CE) of stratified squamous epithelia. -
Tissue specificity
Keratinocytes of epidermis and other stratified squamous epithelia. -
Sequence similarities
Belongs to the involucrin family. -
Post-translational
modificationsSubstrate of transglutaminase. Some glutamines and lysines are cross-linked to other involucrin molecules, to other proteins such as keratin, desmoplakin, periplakin and envoplakin, and to lipids like omega-hydroxyceramide. -
Cellular localization
Cytoplasm. Constituent of the scaffolding of the cornified envelope. - Information by UniProt
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Database links
- Entrez Gene: 3713 Human
- Omim: 147360 Human
- SwissProt: P07476 Human
- Unigene: 516439 Human
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Alternative names
- INVO_HUMAN antibody
- Involucrin antibody
- IVL antibody
Images
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (0)
ab236273 has not yet been referenced specifically in any publications.