Overview

  • Product name

    Anti-Involucrin antibody - N-terminal
    See all Involucrin primary antibodies
  • Description

    Rabbit polyclonal to Involucrin - N-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IF, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Involucrin (N terminal). The exact sequence is proprietary. Conjugated to a protein carrier.
    Database link: P07476

  • Positive control

    • WB: Involucrin transfected HEK-293T whole cell extract. Jurkat, Raji and NCI-H929 whole cell extract. ICC/IF: A431 cells. IHC-P: H661 xenograft.

Properties

Applications

Our Abpromise guarantee covers the use of ab227530 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 68 kDa.
ICC/IF 1/100 - 1/1000.
IHC-P 1/100 - 1/1000.

Target

  • Function

    Part of the insoluble cornified cell envelope (CE) of stratified squamous epithelia.
  • Tissue specificity

    Keratinocytes of epidermis and other stratified squamous epithelia.
  • Sequence similarities

    Belongs to the involucrin family.
  • Post-translational
    modifications

    Substrate of transglutaminase. Some glutamines and lysines are cross-linked to other involucrin molecules, to other proteins such as keratin, desmoplakin, periplakin and envoplakin, and to lipids like omega-hydroxyceramide.
  • Cellular localization

    Cytoplasm. Constituent of the scaffolding of the cornified envelope.
  • Information by UniProt
  • Database links

  • Alternative names

    • INVO_HUMAN antibody
    • Involucrin antibody
    • IVL antibody

Images

  • Paraffin-embedded H661 xenograft stained for Involucrin with ab227530 (1/250 dilution) in immunohistochemical analysis.

  • All lanes : Anti-Involucrin antibody - N-terminal (ab227530) at 1/1000 dilution

    Lane 1 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell extracts
    Lane 2 : Raji (human Burkitt's lymphoma cell line) whole cell extracts
    Lane 3 : NCI-H929 (human bone marrow lymphoblast cell line) whole cell extracts

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG

    Developed using the ECL technique.

    Predicted band size: 68 kDa



    7.5% SDS-PAGE

  • A431 (human epidermoid carcinoma cell line) cells stained for Involucrin (green) using ab227530 (1/200 dilution) in ICC/IF. Cells were fixed in methanol.

    Counterstain: Hoechst 33342 (blue).

  • All lanes : Anti-Involucrin antibody - N-terminal (ab227530) at 1/1000 dilution

    Lane 1 : Non-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extracts
    Lane 2 : Involucrin-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extracts

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG

    Developed using the ECL technique.

    Predicted band size: 68 kDa



    7.5% SDS-PAGE

References

ab227530 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab227530.
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