- Datasheet
- Specific References (24)
- Protocols
Overview
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Product nameAnti-Involucrin antibody [SY5]
See all Involucrin primary antibodies -
DescriptionMouse monoclonal [SY5] to Involucrin
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Host speciesMouse
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Tested applicationsSuitable for: ICC/IF, WB, IP, IHC-P, IHC-Fr, Flow Cytmore details
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Species reactivityReacts with: Dog, Human, Pig, Gorilla, Owl monkey
Does not react with: Mouse -
Immunogen
Pure involucrin from human keratinocytes.
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EpitopeThe epitope maps between codon 421-568 of human involucrin.
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Positive control
- MCF-7 cells. Localized to upper spinous and granular layers in Normal skin.
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General notes
Involucrin is the differentiation marker of human keratinocytes.
ab81216 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityMonoclonal
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Clone numberSY5
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MyelomaP3x63
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IsotypeIgG1
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Light chain typeunknown
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Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab68 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | Use a concentration of 1 µg/ml. | |
| WB | Use at an assay dependent concentration. | |
| IP | Use at an assay dependent concentration. | |
| IHC-P | Use a concentration of 0.1 - 0.2 µg/ml. | |
| IHC-Fr | 1/100. | |
| Flow Cyt | Use 1µg for 106 cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Target
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FunctionPart of the insoluble cornified cell envelope (CE) of stratified squamous epithelia.
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Tissue specificityKeratinocytes of epidermis and other stratified squamous epithelia.
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Sequence similaritiesBelongs to the involucrin family.
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Post-translational
modificationsSubstrate of transglutaminase. Some glutamines and lysines are cross-linked to other involucrin molecules, to other proteins such as keratin, desmoplakin, periplakin and envoplakin, and to lipids like omega-hydroxyceramide. -
Cellular localizationCytoplasm. Constituent of the scaffolding of the cornified envelope.
- Information by UniProt
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Database links
- Entrez Gene: 3713 Human
- Entrez Gene: 407242 Pig
- Omim: 147360 Human
- SwissProt: P07476 Human
- SwissProt: P18175 Pig
- Unigene: 516439 Human
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Alternative names
- INVO_HUMAN antibody
- Involucrin antibody
- IVL antibody
Images
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Involucrin antibody [SY5] (ab68)](https://www.abcam.com/ps/products/0/ab68/Images/ab68-262135-ab68-ihc.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Involucrin antibody [SY5] (ab68)Immunohistochemical analysis of Formalin fixed paraffin embedded human tonsil section labelling Involucrin with ab68 at dilution of 0.1 ug/mL.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Involucrin antibody [SY5] (ab68)](https://www.abcam.com/ps/products/0/ab68/Images/Involucrin-Primary-antibodies-ab68-3.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Involucrin antibody [SY5] (ab68)This image is courtesy of an anonymous abreview.ab68 at a 1/1000 dilution staining Involucrin in human normal skin tissue by Immunohistochemistry (formalin fixed, paraffin embedded sections), incubated for 2 hours at 21°C. Heat mediated antigen retrieval step performed using citrate buffer pH 6.0. Blocked with 10% serum for 30 minutes at 21°C. Secondary used at 1/100 polyclonal Donkey anti-mouse IgG conjugated to Alexa Fluor 594 (red). -
![Immunohistochemistry (Frozen sections) - Anti-Involucrin antibody [SY5] (ab68)](https://www.abcam.com/ps/products/0/ab68/Images/ab68-260888-Involucrin2.jpg)
Immunohistochemistry (Frozen sections) - Anti-Involucrin antibody [SY5] (ab68)Image courtesy of anonymous abreviewerImmunohistochemical analysis of frozen Human artifical skin tissue labeling Involucrin with ab68 at 1/200 dilution.
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![Immunocytochemistry/ Immunofluorescence - Anti-Involucrin antibody [SY5] (ab68)](https://www.abcam.com/ps/products/0/ab68/Images/Involucrin-Primary-antibodies-ab68-1.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Involucrin antibody [SY5] (ab68)ICC/IF image of ab68 stained MCF7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab68, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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![Flow Cytometry - Anti-Involucrin antibody [SY5] (ab68)](https://www.abcam.com/ps/products/0/ab68/Images/ab68-4-ab68FC.jpg)
Flow Cytometry - Anti-Involucrin antibody [SY5] (ab68)Overlay histogram showing A431 cells stained with ab68 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab68, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H&L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in A431 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
Protocols
References
This product has been referenced in:
- Dziunycz PJ et al. CYFIP1 is directly controlled by NOTCH1 and down-regulated in cutaneous squamous cell carcinoma. PLoS One 12:e0173000 (2017). WB ; Human . Read more (PubMed: 28410392) »
- Davies EG et al. Thymus transplantation for complete DiGeorge syndrome: European experience. J Allergy Clin Immunol 140:1660-1670.e16 (2017). IHC-P ; Human . Read more (PubMed: 28400115) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"