Product nameIodoacetamide, Alkylating agent
Alkylating agent used in peptide mapping because it covalently binds with thiols in cysteine residues, thereby preventing disulfide bond formation. By virtue of reaction with cysteine, it is an irreversible inhibitor of enzymes with cysteine at the active site. Also reacts with histidine residues though much more slowly, and this activity is responsible for inhibition of ribonuclease.
Storage instructionsStore at +4°C. The product can be stored for up to 12 months.
Solubility overviewSoluble in water to 100 mM
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
This product has been referenced in:
- Stowell SR et al. Alkylation of galectin-1 with iodoacetamide and mass spectrometric mapping of the sites of incorporation. Methods Mol Biol 1207:51-62 (2015). Read more (PubMed: 25253132) »
- Joliot P & Alric J Inhibition of CO2 fixation by iodoacetamide stimulates cyclic electron flow and non-photochemical quenching upon far-red illumination. Photosynth Res 115:55-63 (2013). Read more (PubMed: 23625532) »
- Ohgi K et al. Alkylation of a ribonuclease from Streptomyces erythreus with iodoacetate and iodoacetamide. J Biochem 90:113-23 (1981). Read more (PubMed: 7287672) »
- Hurlbert RE Effect of thiol-binding reagents on the metabolism of Chromatium D. J Bacteriol 95:1706-12 (1968). Read more (PubMed: 5650077) »