RecombinantRabMAb

Recombinant Anti-Ionotropic glutamate receptor 2 + 3 antibody [EP929Y] (ab52896)

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Western blot - Anti-Ionotropic glutamate receptor 2 + 3 antibody [EP929Y] (ab52896)
  • Western blot - Anti-Ionotropic glutamate receptor 2 + 3 antibody [EP929Y] (ab52896)
  • Western blot - Anti-Ionotropic glutamate receptor 2 + 3 antibody [EP929Y] (ab52896)
  • Anti-Ionotropic glutamate receptor 2 + 3 antibody [EP929Y] (ab52896)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP929Y] to Ionotropic glutamate receptor 2 + 3
  • Suitable for: WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Ionotropic glutamate receptor 2 + 3 antibody [EP929Y]
    See all Ionotropic glutamate receptor 2 + 3 primary antibodies

  • Description

    Rabbit monoclonal [EP929Y] to Ionotropic glutamate receptor 2 + 3

  • Host species

    Rabbit

  • Specificity

    ab52896 detects both GluR2 + 3 phosphorylated on Serine 880/891 of the mature human, and also unphosphorylated GluR2 + 3.

  • Tested Applications & Species

    Application Species
    WB
    Mouse
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human Ionotropic glutamate receptor 2 + 3 (phospho S880 + S891). The exact sequence is proprietary.
    (Peptide available as ab205220)

  • Positive control

    • WB: Human brain and fetal tissue lysates and mouse and rat brain tissue lysates.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab52896 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
WB
Mouse
Human
Application Abreviews Notes
WB
1/2000 - 1/5000. Detects a band of approximately 98 kDa (predicted molecular weight: 98 kDa).
Notes
WB
1/2000 - 1/5000. Detects a band of approximately 98 kDa (predicted molecular weight: 98 kDa).
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF or IHC-P.

    • Target

    • Relevance

      Ion channels activated by glutamate are typically divided into two classes. Those sensitive to N-methyl-D-aspartate (NMDA) are designated NMDA receptors (NMDAR) while those activated by a-amino-3-hydroxy-5-methyl-4-isoxalone propionic acid (AMPA) are known as AMPA receptors (AMPAR). AMPAR are comprised of four distinct Glutamate Receptor Subunits (GluR1-4) and they play key roles in almost all excitatory neurotransmission in the brain. The GluR2 subunit is widely expressed throughout the nervous system where it is thought to play key roles in synaptic plasticity and learning and memory.

    • Cellular localization

      Cell Membrane; multi-pass membrane protein.

    • Database links

      see all

    • Alternative names

      • AMPA selective glutamate receptor 2 antibody
      • AMPA selective glutamate receptor 3 antibody
      • GluR K2 antibody
      • GluR K3 antibody
      • GLURB antibody
      • GLURC antibody
      • GLUTAMATE RECEPTOR 2 antibody
      • GLUTAMATE RECEPTOR 3 antibody
      • Glutamate receptor ionotropic AMPA 2 antibody
      • Glutamate receptor ionotropic AMPA 3 antibody
      • GRIA2 antibody
      • GRIA3 antibody
      see all

    Images

    • Western blot - Anti-Ionotropic glutamate receptor 2 + 3 antibody [EP929Y] (ab52896)
      Western blot - Anti-Ionotropic glutamate receptor 2 + 3 antibody [EP929Y] (ab52896)
      All lanes : Anti-Ionotropic glutamate receptor 2 + 3 antibody [EP929Y] (ab52896) at 1/10000 dilution (purified)

      Lane 1 : Mouse brain tissue lysate
      Lane 2 : Rat brain tissue lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 98 kDa
      Observed band size: 100 kDa why is the actual band size different from the predicted?



      Blocking and dilution buffer: 5% NFDM /TBST.

    • Western blot - Anti-Ionotropic glutamate receptor 2 + 3 antibody [EP929Y] (ab52896)
      Western blot - Anti-Ionotropic glutamate receptor 2 + 3 antibody [EP929Y] (ab52896)
      Anti-Ionotropic glutamate receptor 2 + 3 antibody [EP929Y] (ab52896) at 1/2000 dilution (purified) + Human fetal brain tissue lysate at 10 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 98 kDa
      Observed band size: 100 kDa why is the actual band size different from the predicted?



      Blocking and dilution buffer: 5% NFDM /TBST.

    • Western blot - Anti-Ionotropic glutamate receptor 2 + 3 antibody [EP929Y] (ab52896)
      Western blot - Anti-Ionotropic glutamate receptor 2 + 3 antibody [EP929Y] (ab52896)
      Anti-Ionotropic glutamate receptor 2 + 3 antibody [EP929Y] (ab52896) at 1/5000 dilution (unpurified) + Human brain tissue lysate at 10 µg

      Secondary
      HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution

      Predicted band size: 98 kDa
      Observed band size: 98 kDa

    • Anti-Ionotropic glutamate receptor 2 + 3 antibody [EP929Y] (ab52896)
      Anti-Ionotropic glutamate receptor 2 + 3 antibody [EP929Y] (ab52896)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (6)

    Publishing research using ab52896? Please let us know so that we can cite the reference in this datasheet.

    ab52896 has been referenced in 6 publications.

    • Wei C  et al. MicroRNA-330-3p promotes brain metastasis and epithelial-mesenchymal transition via GRIA3 in non-small cell lung cancer. Aging (Albany NY) 11:6734-6761 (2019). PubMed: 31498117
    • Yang T  et al. Functions of CaBP1 and CaBP2 in the peripheral auditory system. Hear Res 364:48-58 (2018). IHC-P ; Mouse . PubMed: 29661613
    • Zhou JY  et al. Individual and combined effects of rhynchophylline and ketamine on proliferation, NMDAR1 and GluA2/3 protein expression in PC12 cells. Fitoterapia 85:125-9 (2013). ICC/IF ; Rat . PubMed: 23352746
    • Krosnowski K  et al. Diverse populations of intrinsic cholinergic interneurons in the mouse olfactory bulb. Neuroscience 213:161-78 (2012). PubMed: 22525133
    • Ogino K  et al. Duplicated gephyrin genes showing distinct tissue distribution and alternative splicing patterns mediate molybdenum cofactor biosynthesis, glycine receptor clustering, and escape behavior in zebrafish. J Biol Chem 286:806-17 (2011). IHC-Fr ; Zebrafish . PubMed: 20843816
    • Graham CE  et al. A corticotropin-releasing factor system expressed in the cochlea modulates hearing sensitivity and protects against noise-induced hearing loss. Neurobiol Dis 38:246-58 (2010). IP ; Mouse . PubMed: 20109547

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