Recombinant Anti-Ionotropic glutamate receptor 2 + 3 antibody [EP929Y] (ab52896)
- Datasheet
- References (5)
- Protocols
Overview
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Product name
Anti-Ionotropic glutamate receptor 2 + 3 antibody [EP929Y]
See all Ionotropic glutamate receptor 2 + 3 primary antibodies -
Description
Rabbit monoclonal [EP929Y] to Ionotropic glutamate receptor 2 + 3 -
Host species
Rabbit -
Specificity
ab52896 detects both GluR2 + 3 phosphorylated on Serine 880/891 of the mature human, and also unphosphorylated GluR2 + 3. -
Tested applications
Suitable for: WB, IPmore details
Unsuitable for: Flow Cyt,ICC/IF or IHC-P -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human Ionotropic glutamate receptor 2 + 3 (phospho S880 + S891). The exact sequence is proprietary.
(Peptide available asab205220) -
Positive control
- WB: Human brain and fetal tissue lysates and mouse and rat brain tissue lysates.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
This product is a recombinant rabbit monoclonal antibody.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP929Y -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab52896 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/2000 - 1/5000. Detects a band of approximately 98 kDa (predicted molecular weight: 98 kDa).Can be blocked with Ionotropic glutamate receptor 2 + 3 peptide (ab205220). | |
| IP | 1/100. |
Target
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Relevance
Ion channels activated by glutamate are typically divided into two classes. Those sensitive to N-methyl-D-aspartate (NMDA) are designated NMDA receptors (NMDAR) while those activated by a-amino-3-hydroxy-5-methyl-4-isoxalone propionic acid (AMPA) are known as AMPA receptors (AMPAR). AMPAR are comprised of four distinct Glutamate Receptor Subunits (GluR1-4) and they play key roles in almost all excitatory neurotransmission in the brain. The GluR2 subunit is widely expressed throughout the nervous system where it is thought to play key roles in synaptic plasticity and learning and memory. -
Cellular localization
Cell Membrane; multi-pass membrane protein. -
Database links
- Entrez Gene: 2891 Human
- Entrez Gene: 2892 Human
- Entrez Gene: 14800 Mouse
- Entrez Gene: 29627 Rat
- Omim: 138247 Human
- Omim: 305915 Human
- SwissProt: P42262 Human
- SwissProt: P42263 Human
see all -
Alternative names
- AMPA selective glutamate receptor 2 antibody
- AMPA selective glutamate receptor 3 antibody
- GluR K2 antibody
see all
Images
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![Western blot - Anti-Ionotropic glutamate receptor 2 + 3 antibody [EP929Y] (ab52896)](https://www.abcam.com/ps/products/52/ab52896/Images/ab52896-253596-anti-ionotropic-glutamate-receptor-2-3-antibody-ep929y-western-blot.jpg)
Western blot - Anti-Ionotropic glutamate receptor 2 + 3 antibody [EP929Y] (ab52896)All lanes : Anti-Ionotropic glutamate receptor 2 + 3 antibody [EP929Y] (ab52896) at 1/10000 dilution (purified)
Lane 1 : Mouse brain tissue lysate
Lane 2 : Rat brain tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 98 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?Blocking and dilution buffer: 5% NFDM /TBST.
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![Western blot - Anti-Ionotropic glutamate receptor 2 + 3 antibody [EP929Y] (ab52896)](https://www.abcam.com/ps/products/52/ab52896/Images/ab52896-253595-anti-ionotropic-glutamate-receptor-2-3-antibody-ep929y-western-blot.jpg)
Western blot - Anti-Ionotropic glutamate receptor 2 + 3 antibody [EP929Y] (ab52896)Anti-Ionotropic glutamate receptor 2 + 3 antibody [EP929Y] (ab52896) at 1/2000 dilution (purified) + Human fetal brain tissue lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 98 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?Blocking and dilution buffer: 5% NFDM /TBST.
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![Western blot - Anti-Ionotropic glutamate receptor 2 + 3 antibody [EP929Y] (ab52896)](https://www.abcam.com/ps/products/52/ab52896/Images/ab52896-65367-anti-ionotropic-glutamate-receptor-2-3-antibody-ep929y-western-blot.gif)
Western blot - Anti-Ionotropic glutamate receptor 2 + 3 antibody [EP929Y] (ab52896)Anti-Ionotropic glutamate receptor 2 + 3 antibody [EP929Y] (ab52896) at 1/5000 dilution (unpurified) + Human brain tissue lysate at 10 µg
Secondary
HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution
Predicted band size: 98 kDa
Observed band size: 98 kDa
Datasheets and documents
References
This product has been referenced in:
- Yang T et al. Functions of CaBP1 and CaBP2 in the peripheral auditory system. Hear Res 364:48-58 (2018). IHC-P ; Mouse . Read more (PubMed: 29661613) »
- Zhou JY et al. Individual and combined effects of rhynchophylline and ketamine on proliferation, NMDAR1 and GluA2/3 protein expression in PC12 cells. Fitoterapia 85:125-9 (2013). ICC/IF ; Rat . Read more (PubMed: 23352746) »
