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RecombinantRabMAb

Recombinant Anti-Ionotropic glutamate receptor 2 + 3 antibody [EP929Y] - BSA and Azide free (ab247334)

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Western blot - Anti-Ionotropic glutamate receptor 2 + 3 antibody [EP929Y] - BSA and Azide free (ab247334)
  • Western blot - Anti-Ionotropic glutamate receptor 2 + 3 antibody [EP929Y] - BSA and Azide free (ab247334)
  • Western blot - Anti-Ionotropic glutamate receptor 2 + 3 antibody [EP929Y] - BSA and Azide free (ab247334)
  • Anti-Ionotropic glutamate receptor 2 + 3 antibody [EP929Y] - BSA and Azide free (ab247334)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP929Y] to Ionotropic glutamate receptor 2 + 3 - BSA and Azide free
  • Suitable for: WB
  • Reacts with: Mouse, Rat, Human

You may also be interested in

Conjugation
Product image
HRP Conjugation Kit - Lightning-Link® (ab102890)

View more associated products

Overview

  • Product name

    Anti-Ionotropic glutamate receptor 2 + 3 antibody [EP929Y] - BSA and Azide free
    See all Ionotropic glutamate receptor 2 + 3 primary antibodies
  • Description

    Rabbit monoclonal [EP929Y] to Ionotropic glutamate receptor 2 + 3 - BSA and Azide free
  • Host species

    Rabbit
  • Specificity

    This antibody detects both GluR2 + 3 phosphorylated on Serine 880/891 of the mature human, and also unphosphorylated GluR2 + 3.

  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC/IF or IHC-P
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Ionotropic glutamate receptor 2 + 3 (phospho S880 + S891). The exact sequence is proprietary.
    (Peptide available as ab205220)

  • General notes

    Ab247334 is the carrier-free version of ab52896. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab247334 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EP929Y
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Neurotransmission
    • Receptors / Channels
    • GPCR
    • Glutamate Receptors
    • Neuroscience
    • Neurotransmission
    • Receptors / Channels
    • Ligand-Gated Ion Channels
    • AMPA / Kainate

Associated products

  • Alternative Versions

    • Biotin Anti-Ionotropic glutamate receptor 2 + 3 antibody [EP929Y] (ab201534)
    • Anti-Ionotropic glutamate receptor 2 + 3 antibody [EP929Y] (ab52896)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
  • Conjugation kits

    • HRP Conjugation Kit - Lightning-Link® (ab102890)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control - BSA and Azide Free (ab210849)
  • Positive Controls

    • Mouse brain tissue lysate - total protein (ab30151)
    • Mouse brain tissue lysate - total protein (0 days) (ab7188)
    • Mouse brain tissue lysate - total protein (14 days) (ab7189)
    • Mouse brain tissue lysate - total protein (7 days) (ab7190)

Applications

Our Abpromise guarantee covers the use of ab247334 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Detects a band of approximately 98 kDa (predicted molecular weight: 98 kDa).
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF or IHC-P.
  • Target

    • Relevance

      Ion channels activated by glutamate are typically divided into two classes. Those sensitive to N-methyl-D-aspartate (NMDA) are designated NMDA receptors (NMDAR) while those activated by a-amino-3-hydroxy-5-methyl-4-isoxalone propionic acid (AMPA) are known as AMPA receptors (AMPAR). AMPAR are comprised of four distinct Glutamate Receptor Subunits (GluR1-4) and they play key roles in almost all excitatory neurotransmission in the brain. The GluR2 subunit is widely expressed throughout the nervous system where it is thought to play key roles in synaptic plasticity and learning and memory.
    • Cellular localization

      Cell Membrane; multi-pass membrane protein.
    • Database links

      • Entrez Gene: 2891 Human
      • Entrez Gene: 2892 Human
      • Entrez Gene: 14800 Mouse
      • Entrez Gene: 29627 Rat
      • Omim: 138247 Human
      • Omim: 305915 Human
      • SwissProt: P42262 Human
      • SwissProt: P42263 Human
      • SwissProt: P23819 Mouse
      • SwissProt: Q4LG64 Mouse
      • SwissProt: Q80VM6 Mouse
      • SwissProt: P19491 Rat
      • Unigene: 32763 Human
      • Unigene: 377070 Human
      see all
    • Alternative names

      • AMPA selective glutamate receptor 2 antibody
      • AMPA selective glutamate receptor 3 antibody
      • GluR K2 antibody
      • GluR K3 antibody
      • GLURB antibody
      • GLURC antibody
      • GLUTAMATE RECEPTOR 2 antibody
      • GLUTAMATE RECEPTOR 3 antibody
      • Glutamate receptor ionotropic AMPA 2 antibody
      • Glutamate receptor ionotropic AMPA 3 antibody
      • GRIA2 antibody
      • GRIA3 antibody
      see all

    Images

    • Western blot - Anti-Ionotropic glutamate receptor 2 + 3 antibody [EP929Y] - BSA and Azide free (ab247334)
      Western blot - Anti-Ionotropic glutamate receptor 2 + 3 antibody [EP929Y] - BSA and Azide free (ab247334)
      All lanes : Anti-Ionotropic glutamate receptor 2 + 3 antibody [EP929Y] (ab52896) at 1/10000 dilution (purified)

      Lane 1 : Mouse brain tissue lysate
      Lane 2 : Rat brain tissue lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 98 kDa
      Observed band size: 100 kDa
      why is the actual band size different from the predicted?



      This data was developed using ab52896, the same antibody clone in a different buffer formulation.

      Blocking and dilution buffer: 5% NFDM/TBST.

    • Western blot - Anti-Ionotropic glutamate receptor 2 + 3 antibody [EP929Y] - BSA and Azide free (ab247334)
      Western blot - Anti-Ionotropic glutamate receptor 2 + 3 antibody [EP929Y] - BSA and Azide free (ab247334)
      Anti-Ionotropic glutamate receptor 2 + 3 antibody [EP929Y] (ab52896) at 1/2000 dilution (purified) + Human fetal brain tissue lysate at 10 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 98 kDa
      Observed band size: 100 kDa why is the actual band size different from the predicted?



      This data was developed using ab52896, the same antibody clone in a different buffer formulation.

      Blocking and dilution buffer: 5% NFDM/TBST.

    • Western blot - Anti-Ionotropic glutamate receptor 2 + 3 antibody [EP929Y] - BSA and Azide free (ab247334)
      Western blot - Anti-Ionotropic glutamate receptor 2 + 3 antibody [EP929Y] - BSA and Azide free (ab247334)
      Anti-Ionotropic glutamate receptor 2 + 3 antibody [EP929Y] (ab52896) at 1/5000 dilution (unpurified) + Human brain tissue lysate at 10 µg

      Secondary
      HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution

      Predicted band size: 98 kDa
      Observed band size: 98 kDa



      This data was developed using ab52896, the same antibody clone in a different buffer formulation.

    • Anti-Ionotropic glutamate receptor 2 + 3 antibody [EP929Y] - BSA and Azide free (ab247334)
      Anti-Ionotropic glutamate receptor 2 + 3 antibody [EP929Y] - BSA and Azide free (ab247334)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet

    Certificate of Compliance

    To download a Certificate of Compliance, please enter your Lot number below:

  • References (0)

    Publishing research using ab247334? Please let us know so that we can cite the reference in this datasheet.

    ab247334 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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