Product nameAnti-Ionotropic Glutamate receptor 2 antibody [EP966Y]
See all Ionotropic Glutamate receptor 2 primary antibodies
DescriptionRabbit monoclonal [EP966Y] to Ionotropic Glutamate receptor 2
Tested applicationsSuitable for: WB, IP, Flow Cytmore details
Unsuitable for: ICC/IF,IHC-Fr or IHC-P
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human Ionotropic Glutamate receptor 2 aa 1-100 (N terminal). The exact sequence is proprietary.
- WB: Human brain cell lysate. Human, mouse and rat brain lysate.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol, 0.5% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab52932 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000. Detects a band of approximately 110 kDa (predicted molecular weight: 99 kDa).|
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionIonotropic glutamate receptor. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist. In the presence of CACNG4 or CACNG7 or CACNG8, shows resensitization which is characterized by a delayed accumulation of current flux upon continued application of glutamate.
Sequence similaritiesBelongs to the glutamate-gated ion channel (TC 1.A.10.1) family. GRIA2 subfamily.
modificationsPalmitoylated. Depalmitoylated upon glutamate stimulation. Cys-610 palmitoylation leads to Golgi retention and decreased cell surface expression. In contrast, Cys-836 palmitoylation does not affect cell surface expression but regulates stimulation-dependent endocytosis.
Cellular localizationCell membrane. Endoplasmic reticulum membrane. Cell junction > synapse > postsynaptic cell membrane. Interaction with CACNG2, CNIH2 and CNIH3 promotes cell surface expression.
- Information by UniProt
- AMPA 2 antibody
- AMPA selective glutamate receptor 2 antibody
- AMPA-selective glutamate receptor 2 antibody
Anti-Ionotropic Glutamate receptor 2 antibody [EP966Y] (ab52932) at 1/2000 dilution + Human brain cell lysate at 10 µg
Goat anti-Rabbit HRP labeled at 1/2000 dilution
Predicted band size: 99 kDa
Observed band size: 110 kDa why is the actual band size different from the predicted?
This product has been referenced in:
- Huang X et al. Methamphetamine abuse impairs motor cortical plasticity and function. Mol Psychiatry 22:1274-1281 (2017). Read more (PubMed: 28831198) »
- Zhang RX et al. EphB2 in the Medial Prefrontal Cortex Regulates Vulnerability to Stress. Neuropsychopharmacology 41:2541-56 (2016). Read more (PubMed: 27103064) »