Recombinant
RabMAb

Recombinant Anti-Ionotropic Glutamate receptor 2 antibody [EP966Y] - BSA and Azide free (ab246356)

Overview

  • Product name

    Anti-Ionotropic Glutamate receptor 2 antibody [EP966Y] - BSA and Azide free
    See all Ionotropic Glutamate receptor 2 primary antibodies
  • Description

    Rabbit monoclonal [EP966Y] to Ionotropic Glutamate receptor 2 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, Flow Cyt, IPmore details
    Unsuitable for: ICC/IF,IHC-Fr or IHC-P
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Ionotropic Glutamate receptor 2 aa 1-100 (N terminal). The exact sequence is proprietary.

  • Positive control

    • WB: Human brain cell lysate. Human, mouse and rat brain lysate.
  • General notes

    Ab246356 is the carrier-free version of ab52932. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab246356 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab246356 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Detects a band of approximately 110 kDa (predicted molecular weight: 98 kDa).
Flow Cyt Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
  • Application notes
    Is unsuitable for ICC/IF,IHC-Fr or IHC-P.
  • Target

    • Function

      Ionotropic glutamate receptor. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist. In the presence of CACNG4 or CACNG7 or CACNG8, shows resensitization which is characterized by a delayed accumulation of current flux upon continued application of glutamate.
    • Sequence similarities

      Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. GRIA2 subfamily.
    • Post-translational
      modifications

      Palmitoylated. Depalmitoylated upon glutamate stimulation. Cys-610 palmitoylation leads to Golgi retention and decreased cell surface expression. In contrast, Cys-836 palmitoylation does not affect cell surface expression but regulates stimulation-dependent endocytosis.
    • Cellular localization

      Cell membrane. Endoplasmic reticulum membrane. Cell junction > synapse > postsynaptic cell membrane. Interaction with CACNG2, CNIH2 and CNIH3 promotes cell surface expression.
    • Information by UniProt
    • Database links

    • Alternative names

      • AMPA 2 antibody
      • AMPA selective glutamate receptor 2 antibody
      • AMPA-selective glutamate receptor 2 antibody
      • AMPA2 antibody
      • GluA2 antibody
      • GLUR 2 antibody
      • GLUR B antibody
      • GluR K2 antibody
      • GluR-2 antibody
      • GluR-B antibody
      • GluR-K2 antibody
      • GLUR2 antibody
      • GLURB antibody
      • Glutamate receptor 2 antibody
      • Glutamate receptor ionotropic AMPA 2 antibody
      • Glutamate receptor ionotropic antibody
      • Gria2 antibody
      • GRIA2_HUMAN antibody
      • HBGR2 antibody
      see all

    Images

    • All lanes : Anti-Ionotropic Glutamate receptor 2 antibody [EP966Y] (ab52932) at 1/500 dilution

      Lane 1 : Human brain
      Lane 2 : Rat brain
      Lane 3 : Mouse brain

      Predicted band size: 98 kDa



      Secondary antibody - anti-rabbit HRP (ab6721).

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol and sodium azide (ab52932).

    References

    ab246356 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab246356.
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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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