Anti-Ionotropic Glutamate receptor 2 antibody [EPR18115] (ab206293)
- Datasheet
- References (6)
- Protocols
Overview
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Product nameAnti-Ionotropic Glutamate receptor 2 antibody [EPR18115]
See all Ionotropic Glutamate receptor 2 primary antibodies -
DescriptionRabbit monoclonal [EPR18115] to Ionotropic Glutamate receptor 2
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Host speciesRabbit
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Tested applicationsSuitable for: ICC/IF, IP, IHC-P, WBmore details
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Species reactivityReacts with: Mouse, Rat, Human
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Immunogen
Recombinant fragment within Human Ionotropic Glutamate receptor 2 aa 300-450. The exact sequence is proprietary.
Database link: P42262 -
Positive control
- WB: Mouse, rat and Human brain lysates; Human fetal brain lysate. IHC-P: Human solitary fibrous tumor tissue. ICC/IF: HT1080 cells. IP: C6 whole cell lysates.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
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Storage bufferPreservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
PurityProtein A purified
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ClonalityMonoclonal
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Clone numberEPR18115
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IsotypeIgG
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Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Related Products
Applications
Our Abpromise guarantee covers the use of ab206293 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | 1/700. | |
| IP | 1/40. | |
| IHC-P | 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. IHC is recommended for human only. |
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| WB | 1/2000. Detects a band of approximately 99 kDa (predicted molecular weight: 99 kDa). |
Target
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FunctionIonotropic glutamate receptor. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist. In the presence of CACNG4 or CACNG7 or CACNG8, shows resensitization which is characterized by a delayed accumulation of current flux upon continued application of glutamate.
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Sequence similaritiesBelongs to the glutamate-gated ion channel (TC 1.A.10.1) family. GRIA2 subfamily.
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Post-translational
modificationsPalmitoylated. Depalmitoylated upon glutamate stimulation. Cys-610 palmitoylation leads to Golgi retention and decreased cell surface expression. In contrast, Cys-836 palmitoylation does not affect cell surface expression but regulates stimulation-dependent endocytosis. -
Cellular localizationCell membrane. Endoplasmic reticulum membrane. Cell junction > synapse > postsynaptic cell membrane. Interaction with CACNG2, CNIH2 and CNIH3 promotes cell surface expression.
- Information by UniProt
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Database links
- Entrez Gene: 2891 Human
- Entrez Gene: 14800 Mouse
- Entrez Gene: 29627 Rat
- Omim: 138247 Human
- SwissProt: P42262 Human
- SwissProt: P23819 Mouse
- SwissProt: P19491 Rat
- Unigene: 32763 Human
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Alternative names
- AMPA 2 antibody
- AMPA selective glutamate receptor 2 antibody
- AMPA-selective glutamate receptor 2 antibody
see all
Images
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![Western blot - Anti-Ionotropic Glutamate receptor 2 antibody [EPR18115] (ab206293)](https://www.abcam.com/ps/products/206/ab206293/Images/ab206293-253187-206293WBa.jpg)
Western blot - Anti-Ionotropic Glutamate receptor 2 antibody [EPR18115] (ab206293)All lanes : Anti-Ionotropic Glutamate receptor 2 antibody [EPR18115] (ab206293) at 1/5000 dilution
Lane 1 : Mouse brain lysate
Lane 2 : Rat brain lysate
Lane 3 : Human fetal brain lysate
Lane 4 : Rat spleen lysate
Lane 5 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 6 : Human fetal kidney lysate
Lane 7 : HT1080 (Human fibrosarcoma cell line) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution
Predicted band size: 99 kDa
Observed band size: 99 kDaBlocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: Lane 1: 1 second; Lane 2,3,4,5,6 and 7: 10 seconds.
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![Western blot - Anti-Ionotropic Glutamate receptor 2 antibody [EPR18115] (ab206293)](https://www.abcam.com/ps/products/206/ab206293/Images/ab206293-253185-ab206293.jpg)
Western blot - Anti-Ionotropic Glutamate receptor 2 antibody [EPR18115] (ab206293)All lanes : Anti-Ionotropic Glutamate receptor 2 antibody [EPR18115] (ab206293) at 1/2000 dilution
Lane 1 : Human brain lysate
Lane 2 : Mouse brain lysate
Lane 3 : Rat brain lysate
Lane 4 : C6 (Rat glial tumor cell line) whole cell lysate
Lane 5 : Human fetal kidney lysate
Lane 6 : HT1080 (Human fibrosarcoma cell line) whole cell lysate
Lane 7 : Mouse heart lysate
Lane 8 : Rat spleen lysate
Lane 9 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution
Predicted band size: 99 kDa
Observed band size: 99 kDa
Exposure time: 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ionotropic Glutamate receptor 2 antibody [EPR18115] (ab206293)](https://www.abcam.com/ps/products/206/ab206293/Images/ab206293-253183-206293IHC.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ionotropic Glutamate receptor 2 antibody [EPR18115] (ab206293)Immunohistochemical analysis of paraffin-embedded Human solitary fibrous tumor tissue labeling Ionotropic Glutamate receptor 2 with ab206293 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Membrane staining on Human solitary fibrous tumor is observed.
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
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![Immunocytochemistry/ Immunofluorescence - Anti-Ionotropic Glutamate receptor 2 antibody [EPR18115] (ab206293)](https://www.abcam.com/ps/products/206/ab206293/Images/ab206293-253175-206293IF.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Ionotropic Glutamate receptor 2 antibody [EPR18115] (ab206293)Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HT1080 (Human fibrosarcoma cell line) cells labeling Ionotropic Glutamate receptor 2 with ab206293 at 1/700 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).
Confocal image showing cytoplasmic staining on HT1080 cell line.
The nuclear counterstain is DAPI (blue).
Tubulin is detected with Anti-alpha Tubulin antibody [EPR18115] - Loading Control (ab7291) at 1/1000 dilution and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed (ab150120) at 1/1000 dilution (red).
The negative controls are as follows:-
-ve control 1: ab206293 at 1/700 dilution followed by ab150120 at 1/1000 dilution.
-ve control 2: ab7291 at 1/1000 dilution followed by ab150077 at 1/1000 dilution.
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![Immunoprecipitation - Anti-Ionotropic Glutamate receptor 2 antibody [EPR18115] (ab206293)](https://www.abcam.com/ps/products/206/ab206293/Images/ab206293-253173-206293IP.jpg)
Immunoprecipitation - Anti-Ionotropic Glutamate receptor 2 antibody [EPR18115] (ab206293)Ionotropic Glutamate receptor 2 was immunoprecipitated from 1mg of C6 (Rat glial tumor cell line) whole cell lysate with ab206293 at 1/40 dilution.
Western blot was performed from the immunoprecipitate using ab206293 at 1/1000 dilution.
VeriBlot for IP secondary antibody (HRP) (ab131366), was used as secondary antibody at 1/10000 dilution.
Lane 1: C6 whole cell lysate 10µg (Input).
Lane 2: ab206293 IP in C6 whole cell lysate.
Lane 3:Rabbit IgG, monoclonal [EPR18115] - Isotype Control (ab172730) instead of ab206293 in C6 whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 10 seconds.
Protocols
Datasheets and documents
References
This product has been referenced in:
- Wong JH et al. Differential expression of entorhinal cortex and hippocampal subfields a-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) and N-methyl-D-aspartate (NMDA) receptors enhanced learning and memory of rats following administration of Centella asiatica. Biomed Pharmacother 110:168-180 (2019). Read more (PubMed: 30469081) »
- Yu X et al. Endophilin A1 mediates seizure activity via regulation of AMPARs in a PTZ-kindled epileptic mouse model. Exp Neurol 304:41-57 (2018). WB . Read more (PubMed: 29481784) »
