Recombinant
RabMAb

Recombinant Anti-Ionotropic Glutamate receptor 2 antibody [EPR18115] (ab206293)

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Overview

  • Product name

    Anti-Ionotropic Glutamate receptor 2 antibody [EPR18115]
    See all Ionotropic Glutamate receptor 2 primary antibodies

  • Description

    Rabbit monoclonal [EPR18115] to Ionotropic Glutamate receptor 2

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC/IF, IP, IHC-P, WBmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment within Human Ionotropic Glutamate receptor 2 aa 300-450. The exact sequence is proprietary.
    Database link: P42262

  • Positive control

    • WB: Mouse, rat and Human brain lysates; Human fetal brain lysate. IHC-P: Human solitary fibrous tumor tissue. ICC/IF: HT1080 cells. IP: C6 whole cell lysates.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

Our Abpromise guarantee covers the use of ab206293 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/700.
IP 1/40.
IHC-P 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

IHC is recommended for human only.
WB 1/2000. Detects a band of approximately 99 kDa (predicted molecular weight: 99 kDa).

Target

  • Function

    Ionotropic glutamate receptor. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist. In the presence of CACNG4 or CACNG7 or CACNG8, shows resensitization which is characterized by a delayed accumulation of current flux upon continued application of glutamate.

  • Sequence similarities

    Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. GRIA2 subfamily.

  • Post-translational
    modifications

    Palmitoylated. Depalmitoylated upon glutamate stimulation. Cys-610 palmitoylation leads to Golgi retention and decreased cell surface expression. In contrast, Cys-836 palmitoylation does not affect cell surface expression but regulates stimulation-dependent endocytosis.

  • Cellular localization

    Cell membrane. Endoplasmic reticulum membrane. Cell junction > synapse > postsynaptic cell membrane. Interaction with CACNG2, CNIH2 and CNIH3 promotes cell surface expression.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • AMPA 2 antibody
    • AMPA selective glutamate receptor 2 antibody
    • AMPA-selective glutamate receptor 2 antibody
    • AMPA2 antibody
    • GluA2 antibody
    • GLUR 2 antibody
    • GLUR B antibody
    • GluR K2 antibody
    • GluR-2 antibody
    • GluR-B antibody
    • GluR-K2 antibody
    • GLUR2 antibody
    • GLURB antibody
    • Glutamate receptor 2 antibody
    • Glutamate receptor ionotropic AMPA 2 antibody
    • Glutamate receptor ionotropic antibody
    • Gria2 antibody
    • GRIA2_HUMAN antibody
    • HBGR2 antibody
    see all

Images

  • Western blot - Anti-Ionotropic Glutamate receptor 2 antibody [EPR18115] (ab206293)
    Western blot - Anti-Ionotropic Glutamate receptor 2 antibody [EPR18115] (ab206293)
    All lanes : Anti-Ionotropic Glutamate receptor 2 antibody [EPR18115] (ab206293) at 1/5000 dilution

    Lane 1 : Mouse brain lysate
    Lane 2 : Rat brain lysate
    Lane 3 : Human fetal brain lysate
    Lane 4 : Rat spleen lysate
    Lane 5 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 6 : Human fetal kidney lysate
    Lane 7 : HT1080 (Human fibrosarcoma cell line) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution

    Predicted band size: 99 kDa
    Observed band size: 99 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: Lane 1: 1 second; Lane 2,3,4,5,6 and 7: 10 seconds.

  • Western blot - Anti-Ionotropic Glutamate receptor 2 antibody [EPR18115] (ab206293)
    Western blot - Anti-Ionotropic Glutamate receptor 2 antibody [EPR18115] (ab206293)
    All lanes : Anti-Ionotropic Glutamate receptor 2 antibody [EPR18115] (ab206293) at 1/2000 dilution

    Lane 1 : Human brain lysate
    Lane 2 : Mouse brain lysate
    Lane 3 : Rat brain lysate
    Lane 4 : C6 (Rat glial tumor cell line) whole cell lysate
    Lane 5 : Human fetal kidney lysate
    Lane 6 : HT1080 (Human fibrosarcoma cell line) whole cell lysate
    Lane 7 : Mouse heart lysate
    Lane 8 : Rat spleen lysate
    Lane 9 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution

    Predicted band size: 99 kDa
    Observed band size: 99 kDa


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ionotropic Glutamate receptor 2 antibody [EPR18115] (ab206293)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ionotropic Glutamate receptor 2 antibody [EPR18115] (ab206293)

    Immunohistochemical analysis of paraffin-embedded Human solitary fibrous tumor tissue labeling Ionotropic Glutamate receptor 2 with ab206293 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Membrane staining on Human solitary fibrous tumor is observed.

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-Ionotropic Glutamate receptor 2 antibody [EPR18115] (ab206293)
    Immunocytochemistry/ Immunofluorescence - Anti-Ionotropic Glutamate receptor 2 antibody [EPR18115] (ab206293)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HT1080 (Human fibrosarcoma cell line) cells labeling Ionotropic Glutamate receptor 2 with ab206293 at 1/700 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

     

    Confocal image showing cytoplasmic staining on HT1080 cell line.

     

    The nuclear counterstain is DAPI (blue).

     

    Tubulin is detected with Anti-alpha Tubulin antibody [EPR18115] - Loading Control (ab7291) at 1/1000 dilution and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed (ab150120) at 1/1000 dilution (red).

     

    The negative controls are as follows:-

    -ve control 1: ab206293 at 1/700 dilution followed by ab150120  at 1/1000 dilution.

    -ve control 2: ab7291  at 1/1000 dilution followed by ab150077  at 1/1000 dilution.

  • Immunoprecipitation - Anti-Ionotropic Glutamate receptor 2 antibody [EPR18115] (ab206293)
    Immunoprecipitation - Anti-Ionotropic Glutamate receptor 2 antibody [EPR18115] (ab206293)

    Ionotropic Glutamate receptor 2 was immunoprecipitated from 1mg of C6 (Rat glial tumor cell line) whole cell lysate with ab206293 at 1/40 dilution.

    Western blot was performed from the immunoprecipitate using ab206293 at 1/1000 dilution.

    VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

    Lane 1: C6 whole cell lysate 10µg (Input).

    Lane 2: ab206293 IP in C6 whole cell lysate.

    Lane 3:Rabbit IgG, monoclonal [EPR18115] - Isotype Control (ab172730) instead of ab206293 in C6 whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 10 seconds.

References

This product has been referenced in:

  • Wong JH  et al. Differential expression of entorhinal cortex and hippocampal subfields a-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) and N-methyl-D-aspartate (NMDA) receptors enhanced learning and memory of rats following administration of Centella asiatica. Biomed Pharmacother 110:168-180 (2019). Read more (PubMed: 30469081) »
  • Tabassum S  et al. Jujuboside A prevents sleep loss-induced disturbance of hippocampal neuronal excitability and memory impairment in young APP/PS1 mice. Sci Rep 9:4512 (2019). Read more (PubMed: 30872728) »
See all 8 Publications for this product

Publishing research using ab206293? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Ionotropic Glutamate receptor 2 antibody [EPR18115]

 Inconclusive
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Dog Tissue sections (cranial cruciate ligament, meniscus and brain)
Antigen retrieval step
Enzymatic - Buffer/Enzyme Used: trypsin from porcine pancreas
Permeabilization
No
Specification
cranial cruciate ligament, meniscus and brain
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
Fixative
Formaldehyde
Read More

Dr. Joel Alves

Verified customer

Submitted Jan 11 2019

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