Recombinant Anti-Ionotropic Glutamate receptor 2 antibody [EPR18115] (ab206293)
![Western blot - Anti-Ionotropic Glutamate receptor 2 antibody [EPR18115] (ab206293)](https://www.abcam.com/ps/products/206/ab206293/Images/ab206293-253187-anti-ionotropic-glutamate-receptor-2-antibody-epr18115-western-blot.jpg "Western blot - Anti-Ionotropic Glutamate receptor 2 antibody [EPR18115] (ab206293)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR18115] to Ionotropic Glutamate receptor 2
- Suitable for: IP, IHC-P, WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-Ionotropic Glutamate receptor 2 antibody [EPR18115]
See all Ionotropic Glutamate receptor 2 primary antibodies -
Description
Rabbit monoclonal [EPR18115] to Ionotropic Glutamate receptor 2 -
Host species
Rabbit -
Tested applications
Suitable for: IP, IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Mouse, rat and Human brain lysates; Human fetal brain lysate. IHC-P: Human solitary fibrous tumor tissue. IP: C6 whole cell lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR18115 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab206293 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IP |
1/40.
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| IHC-P |
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IHC is recommended for human only. |
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| WB | (2) |
1/2000. Detects a band of approximately 99 kDa (predicted molecular weight: 99 kDa).
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| Notes |
|---|
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IP
1/40. |
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IHC-P
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. IHC is recommended for human only. |
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WB
1/2000. Detects a band of approximately 99 kDa (predicted molecular weight: 99 kDa). |
Target
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Function
Ionotropic glutamate receptor. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist. In the presence of CACNG4 or CACNG7 or CACNG8, shows resensitization which is characterized by a delayed accumulation of current flux upon continued application of glutamate. -
Sequence similarities
Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. GRIA2 subfamily. -
Post-translational
modificationsPalmitoylated. Depalmitoylated upon glutamate stimulation. Cys-610 palmitoylation leads to Golgi retention and decreased cell surface expression. In contrast, Cys-836 palmitoylation does not affect cell surface expression but regulates stimulation-dependent endocytosis. -
Cellular localization
Cell membrane. Endoplasmic reticulum membrane. Cell junction > synapse > postsynaptic cell membrane. Interaction with CACNG2, CNIH2 and CNIH3 promotes cell surface expression. - Information by UniProt
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Database links
- Entrez Gene: 2891 Human
- Entrez Gene: 14800 Mouse
- Entrez Gene: 29627 Rat
- Omim: 138247 Human
- SwissProt: P42262 Human
- SwissProt: P23819 Mouse
- SwissProt: P19491 Rat
- Unigene: 32763 Human
see all -
Alternative names
- AMPA 2 antibody
- AMPA selective glutamate receptor 2 antibody
- AMPA-selective glutamate receptor 2 antibody
see all
Images
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Western blot - Anti-Ionotropic Glutamate receptor 2 antibody [EPR18115] (ab206293)All lanes : Anti-Ionotropic Glutamate receptor 2 antibody [EPR18115] (ab206293) at 1/5000 dilution
Lane 1 : Mouse brain lysate
Lane 2 : Rat brain lysate
Lane 3 : Human fetal brain lysate
Lane 4 : Rat spleen lysate
Lane 5 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 6 : Human fetal kidney lysate
Lane 7 : HT1080 (Human fibrosarcoma cell line) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution
Predicted band size: 99 kDa
Observed band size: 99 kDaBlocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: Lane 1: 1 second; Lane 2,3,4,5,6 and 7: 10 seconds.
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![Western blot - Anti-Ionotropic Glutamate receptor 2 antibody [EPR18115] (ab206293)](https://www.abcam.com/ps/products/206/ab206293/Images/ab206293-253185-anti-ionotropic-glutamate-receptor-2-antibody-epr18115-western-blot.jpg)
Western blot - Anti-Ionotropic Glutamate receptor 2 antibody [EPR18115] (ab206293)All lanes : Anti-Ionotropic Glutamate receptor 2 antibody [EPR18115] (ab206293) at 1/2000 dilution
Lane 1 : Human brain lysate
Lane 2 : Mouse brain lysate
Lane 3 : Rat brain lysate
Lane 4 : C6 (Rat glial tumor cell line) whole cell lysate
Lane 5 : Human fetal kidney lysate
Lane 6 : HT1080 (Human fibrosarcoma cell line) whole cell lysate
Lane 7 : Mouse heart lysate
Lane 8 : Rat spleen lysate
Lane 9 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution
Predicted band size: 99 kDa
Observed band size: 99 kDa
Exposure time: 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ionotropic Glutamate receptor 2 antibody [EPR18115] (ab206293)](https://www.abcam.com/ps/products/206/ab206293/Images/ab206293-253183-anti-ionotropic-glutamate-receptor-2-antibody-epr18115-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ionotropic Glutamate receptor 2 antibody [EPR18115] (ab206293)Immunohistochemical analysis of paraffin-embedded Human solitary fibrous tumor tissue labeling Ionotropic Glutamate receptor 2 with ab206293 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Membrane staining on Human solitary fibrous tumor is observed.
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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![Immunoprecipitation - Anti-Ionotropic Glutamate receptor 2 antibody [EPR18115] (ab206293)](https://www.abcam.com/ps/products/206/ab206293/Images/ab206293-253173-anti-ionotropic-glutamate-receptor-2-antibody-epr18115-immunoprecipitation.jpg)
Immunoprecipitation - Anti-Ionotropic Glutamate receptor 2 antibody [EPR18115] (ab206293)Ionotropic Glutamate receptor 2 was immunoprecipitated from 1mg of C6 (Rat glial tumor cell line) whole cell lysate with ab206293 at 1/40 dilution.
Western blot was performed from the immunoprecipitate using ab206293 at 1/1000 dilution.
VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.
Lane 1: C6 whole cell lysate 10µg (Input).
Lane 2: ab206293 IP in C6 whole cell lysate.
Lane 3:Rabbit IgG, monoclonal [EPR18115] - Isotype Control (ab172730) instead of ab206293 in C6 whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 10 seconds.
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![Anti-Ionotropic Glutamate receptor 2 antibody [EPR18115] (ab206293)](https://www.abcam.com/ps/products/206/ab206293/Images/ab206293-7-benefits-of-recombinant-antibodies.png)
Anti-Ionotropic Glutamate receptor 2 antibody [EPR18115] (ab206293)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (15)
ab206293 has been referenced in 15 publications.
- Li J et al. Protection of hUC-MSCs against neuronal complement C3a receptor-mediated NLRP3 activation in CUMS-induced mice. Neurosci Lett 741:135485 (2021). PubMed: 33161108
- González-Calvo I et al. Sushi domain-containing protein 4 controls synaptic plasticity and motor learning. Elife 10:N/A (2021). PubMed: 33661101
- Chen M et al. Enhancing GABAergic signaling ameliorates aberrant gamma oscillations of olfactory bulb in AD mouse models. Mol Neurodegener 16:14 (2021). PubMed: 33663578
- Cisani F et al. Antibodies Against the NH2-Terminus of the GluA Subunits Affect the AMPA-Evoked Releasing Activity: The Role of Complement. Front Immunol 12:586521 (2021). PubMed: 33717067
- Verhaeghe R et al. Maternal stress programs a demasculinization of glutamatergic transmission in stress-related brain regions of aged rats. Geroscience N/A:N/A (2021). PubMed: 33983623