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  1. Link

    ionotropic-glutamate-receptor-2-antibody-epr18115-ab206293.pdf

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Neuroscience Neurotransmitter Amino Acids Glutamate
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RecombinantRabMAb

Recombinant Anti-Ionotropic Glutamate receptor 2 antibody [EPR18115] (ab206293)

  • Datasheet
  • SDS
  • Certificate of Compliance
Reviews (3) Submit a question References (15)

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Western blot - Anti-Ionotropic Glutamate receptor 2 antibody [EPR18115] (ab206293)
  • Western blot - Anti-Ionotropic Glutamate receptor 2 antibody [EPR18115] (ab206293)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ionotropic Glutamate receptor 2 antibody [EPR18115] (ab206293)
  • Immunoprecipitation - Anti-Ionotropic Glutamate receptor 2 antibody [EPR18115] (ab206293)
  • Anti-Ionotropic Glutamate receptor 2 antibody [EPR18115] (ab206293)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR18115] to Ionotropic Glutamate receptor 2
  • Suitable for: IP, IHC-P, WB
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

You may also be interested in

Primary
Product image
Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR5479] (ab109450)
Secondary
Product image
Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
Peptide
Human PSMA peptide (ab41442)

View more associated products

Overview

  • Product name

    Anti-Ionotropic Glutamate receptor 2 antibody [EPR18115]
    See all Ionotropic Glutamate receptor 2 primary antibodies
  • Description

    Rabbit monoclonal [EPR18115] to Ionotropic Glutamate receptor 2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IP, IHC-P, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Mouse, rat and Human brain lysates; Human fetal brain lysate. IHC-P: Human solitary fibrous tumor tissue. IP: C6 whole cell lysates.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR18115
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Neurotransmitter
    • Amino Acids
    • Glutamate
    • Neuroscience
    • Neurotransmission
    • Receptors / Channels
    • GPCR
    • Glutamate Receptors
    • Neuroscience
    • Processes

Associated products

  • Alternative Versions

    • Anti-Ionotropic Glutamate receptor 2 antibody [EPR18115] - BSA and Azide free (ab251444)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control - BSA and Azide Free (ab210849)
  • Related Products

    • VeriBlot for IP Detection Reagent (HRP) (ab131366)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab206293 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP
1/40.
IHC-P
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

IHC is recommended for human only.

WB (2)
1/2000. Detects a band of approximately 99 kDa (predicted molecular weight: 99 kDa).
Notes
IP
1/40.
IHC-P
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

IHC is recommended for human only.

WB
1/2000. Detects a band of approximately 99 kDa (predicted molecular weight: 99 kDa).

Target

  • Function

    Ionotropic glutamate receptor. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist. In the presence of CACNG4 or CACNG7 or CACNG8, shows resensitization which is characterized by a delayed accumulation of current flux upon continued application of glutamate.
  • Sequence similarities

    Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. GRIA2 subfamily.
  • Post-translational
    modifications

    Palmitoylated. Depalmitoylated upon glutamate stimulation. Cys-610 palmitoylation leads to Golgi retention and decreased cell surface expression. In contrast, Cys-836 palmitoylation does not affect cell surface expression but regulates stimulation-dependent endocytosis.
  • Cellular localization

    Cell membrane. Endoplasmic reticulum membrane. Cell junction > synapse > postsynaptic cell membrane. Interaction with CACNG2, CNIH2 and CNIH3 promotes cell surface expression.
  • Target information above from: UniProt accession P42262 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 2891 Human
    • Entrez Gene: 14800 Mouse
    • Entrez Gene: 29627 Rat
    • Omim: 138247 Human
    • SwissProt: P42262 Human
    • SwissProt: P23819 Mouse
    • SwissProt: P19491 Rat
    • Unigene: 32763 Human
    • Unigene: 220224 Mouse
    • Unigene: 91361 Rat
    see all
  • Alternative names

    • AMPA 2 antibody
    • AMPA selective glutamate receptor 2 antibody
    • AMPA-selective glutamate receptor 2 antibody
    • AMPA2 antibody
    • GluA2 antibody
    • GLUR 2 antibody
    • GLUR B antibody
    • GluR K2 antibody
    • GluR-2 antibody
    • GluR-B antibody
    • GluR-K2 antibody
    • GLUR2 antibody
    • GLURB antibody
    • Glutamate receptor 2 antibody
    • Glutamate receptor ionotropic AMPA 2 antibody
    • Glutamate receptor ionotropic antibody
    • Gria2 antibody
    • GRIA2_HUMAN antibody
    • HBGR2 antibody
    see all

Images

  • Western blot - Anti-Ionotropic Glutamate receptor 2 antibody [EPR18115] (ab206293)
    Western blot - Anti-Ionotropic Glutamate receptor 2 antibody [EPR18115] (ab206293)
    All lanes : Anti-Ionotropic Glutamate receptor 2 antibody [EPR18115] (ab206293) at 1/5000 dilution

    Lane 1 : Mouse brain lysate
    Lane 2 : Rat brain lysate
    Lane 3 : Human fetal brain lysate
    Lane 4 : Rat spleen lysate
    Lane 5 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 6 : Human fetal kidney lysate
    Lane 7 : HT1080 (Human fibrosarcoma cell line) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution

    Predicted band size: 99 kDa
    Observed band size: 99 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: Lane 1: 1 second; Lane 2,3,4,5,6 and 7: 10 seconds.

  • Western blot - Anti-Ionotropic Glutamate receptor 2 antibody [EPR18115] (ab206293)
    Western blot - Anti-Ionotropic Glutamate receptor 2 antibody [EPR18115] (ab206293)
    All lanes : Anti-Ionotropic Glutamate receptor 2 antibody [EPR18115] (ab206293) at 1/2000 dilution

    Lane 1 : Human brain lysate
    Lane 2 : Mouse brain lysate
    Lane 3 : Rat brain lysate
    Lane 4 : C6 (Rat glial tumor cell line) whole cell lysate
    Lane 5 : Human fetal kidney lysate
    Lane 6 : HT1080 (Human fibrosarcoma cell line) whole cell lysate
    Lane 7 : Mouse heart lysate
    Lane 8 : Rat spleen lysate
    Lane 9 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution

    Predicted band size: 99 kDa
    Observed band size: 99 kDa


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ionotropic Glutamate receptor 2 antibody [EPR18115] (ab206293)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ionotropic Glutamate receptor 2 antibody [EPR18115] (ab206293)

    Immunohistochemical analysis of paraffin-embedded Human solitary fibrous tumor tissue labeling Ionotropic Glutamate receptor 2 with ab206293 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Membrane staining on Human solitary fibrous tumor is observed.

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunoprecipitation - Anti-Ionotropic Glutamate receptor 2 antibody [EPR18115] (ab206293)
    Immunoprecipitation - Anti-Ionotropic Glutamate receptor 2 antibody [EPR18115] (ab206293)

    Ionotropic Glutamate receptor 2 was immunoprecipitated from 1mg of C6 (Rat glial tumor cell line) whole cell lysate with ab206293 at 1/40 dilution.

    Western blot was performed from the immunoprecipitate using ab206293 at 1/1000 dilution.

    VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

    Lane 1: C6 whole cell lysate 10µg (Input).

    Lane 2: ab206293 IP in C6 whole cell lysate.

    Lane 3:Rabbit IgG, monoclonal [EPR18115] - Isotype Control (ab172730) instead of ab206293 in C6 whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 10 seconds.

  • Anti-Ionotropic Glutamate receptor 2 antibody [EPR18115] (ab206293)
    Anti-Ionotropic Glutamate receptor 2 antibody [EPR18115] (ab206293)

Protocols

  • Immunoprecipitation protocols
  • Immunohistochemistry protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

References (15)

Publishing research using ab206293? Please let us know so that we can cite the reference in this datasheet.

ab206293 has been referenced in 15 publications.

  • Li J  et al. Protection of hUC-MSCs against neuronal complement C3a receptor-mediated NLRP3 activation in CUMS-induced mice. Neurosci Lett 741:135485 (2021). PubMed: 33161108
  • González-Calvo I  et al. Sushi domain-containing protein 4 controls synaptic plasticity and motor learning. Elife 10:N/A (2021). PubMed: 33661101
  • Chen M  et al. Enhancing GABAergic signaling ameliorates aberrant gamma oscillations of olfactory bulb in AD mouse models. Mol Neurodegener 16:14 (2021). PubMed: 33663578
  • Cisani F  et al. Antibodies Against the NH2-Terminus of the GluA Subunits Affect the AMPA-Evoked Releasing Activity: The Role of Complement. Front Immunol 12:586521 (2021). PubMed: 33717067
  • Verhaeghe R  et al. Maternal stress programs a demasculinization of glutamatergic transmission in stress-related brain regions of aged rats. Geroscience N/A:N/A (2021). PubMed: 33983623
View all Publications for this product

Customer reviews and Q&As

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Submit a review Submit a question

1-2 of 2 Abreviews or Q&A

Western blot abreview for Anti-Ionotropic Glutamate receptor 2 antibody [EPR18115]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (293 HEK cells trasfected with human GluA2)
Gel Running Conditions
Reduced Denaturing (10%)
Loading amount
20 µg
Specification
293 HEK cells trasfected with human GluA2
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted May 11 2020

Western blot abreview for Anti-Ionotropic Glutamate receptor 2 antibody [EPR18115]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Cell lysate - other (mouse cultured neurons)
Gel Running Conditions
Reduced Denaturing (10% gel)
Loading amount
20 µg
Specification
mouse cultured neurons
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted May 04 2020

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