Recombinant
RabMAb

Recombinant Anti-Ionotropic Glutamate receptor 2 antibody [EPR5032] (ab133477)

Rabbit recombinant monoclonal Ionotropic Glutamate receptor 2 antibody [EPR5032]. Validated in WB, IP, Flow Cyt, ICC/IF and tested in Mouse, Rat, Human. Cited in 7 publication(s).

Overview

  • Product name
    Anti-Ionotropic Glutamate receptor 2 antibody [EPR5032]
    See all Ionotropic Glutamate receptor 2 primary antibodies
  • Description
    Rabbit monoclonal [EPR5032] to Ionotropic Glutamate receptor 2
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IP, Flow Cyt, WB, ICC/IFmore details
    Unsuitable for: IHC-P
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Ionotropic Glutamate receptor 2 aa 150-250. The exact sequence is proprietary.

  • Positive control
    • WB: Human fetal brain, adult brain, hippocampus, hypothalamus, and midbrain tissue lysates. ICC/IF: SK-N-SH cells. Flow Cyt: SH-SY5Y cells.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab133477 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP 1/10 - 1/100.
Flow Cyt 1/200.
WB 1/1000 - 1/10000. Predicted molecular weight: 98 kDa.
ICC/IF 1/100 - 1/250.
  • Application notes
    Is unsuitable for IHC-P.
  • Target

    • Function
      Ionotropic glutamate receptor. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist. In the presence of CACNG4 or CACNG7 or CACNG8, shows resensitization which is characterized by a delayed accumulation of current flux upon continued application of glutamate.
    • Sequence similarities
      Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. GRIA2 subfamily.
    • Post-translational
      modifications
      Palmitoylated. Depalmitoylated upon glutamate stimulation. Cys-610 palmitoylation leads to Golgi retention and decreased cell surface expression. In contrast, Cys-836 palmitoylation does not affect cell surface expression but regulates stimulation-dependent endocytosis.
    • Cellular localization
      Cell membrane. Endoplasmic reticulum membrane. Cell junction > synapse > postsynaptic cell membrane. Interaction with CACNG2, CNIH2 and CNIH3 promotes cell surface expression.
    • Information by UniProt
    • Database links
    • Alternative names
      • AMPA 2 antibody
      • AMPA selective glutamate receptor 2 antibody
      • AMPA-selective glutamate receptor 2 antibody
      • AMPA2 antibody
      • GluA2 antibody
      • GLUR 2 antibody
      • GLUR B antibody
      • GluR K2 antibody
      • GluR-2 antibody
      • GluR-B antibody
      • GluR-K2 antibody
      • GLUR2 antibody
      • GLURB antibody
      • Glutamate receptor 2 antibody
      • Glutamate receptor ionotropic AMPA 2 antibody
      • Glutamate receptor ionotropic antibody
      • Gria2 antibody
      • GRIA2_HUMAN antibody
      • HBGR2 antibody
      see all

    Images

    • All lanes : Anti-Ionotropic Glutamate receptor 2 antibody [EPR5032] (ab133477) at 1/1000 dilution

      Lane 1 : Fetal brain lysate
      Lane 2 : Human brain lysate
      Lane 3 : Human hippocampus lysate
      Lane 4 : Human hypothalamus lysate
      Lane 5 : Human midbrain lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 98 kDa

    • ab133477 staining GluR2 in SK-N-SH cells treated with VU 0360223 (ab120803), by ICC/IF. Decrease of GluR2 expression correlates with increased concentration of VU 0360223, as described in literature.
      The cells were incubated at 37°C for 6h in media containing different concentrations of ab120803 (VU 0360223) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab133477 (1/200 dilution was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
    • Flow cytometry analysis of SH-SY5Y ( Human neuroblastoma epithelial cell) cells labeling Ionotropic Glutamate receptor 2 (red) with ab133477 at a 1/200 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (Black) (ab172730). Blue (unlabeled control) - Cell without incubation with primary antibody and secondary antibody (Blue).

    References

    This product has been referenced in:
    • Lum JS  et al. Chronic Adolescent CDPPB Treatment Alters Short-Term, but not Long-Term, Glutamatergic Receptor Expression. Neurochem Res N/A:N/A (2018). Read more (PubMed: 29936568) »
    • Zhang Z  et al. METTL3-mediated N6-methyladenosine mRNA modification enhances long-term memory consolidation. Cell Res N/A:N/A (2018). WB ; Mouse . Read more (PubMed: 30297870) »
    See all 7 Publications for this product

    Customer reviews and Q&As

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    Application
    Western blot
    Loading amount
    10 µg
    Gel Running Conditions
    Reduced Denaturing (4-20%)
    Sample
    Mouse Cell lysate - whole cell (Oligodendrocyte- GluR2 WT and Null)
    Specification
    Oligodendrocyte- GluR2 WT and Null
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C

    Ms. Katherine Given

    Verified customer

    Submitted Mar 17 2015

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