Western blot abreview for Anti-Ionotropic Glutamate receptor 2 antibody [EPR5032]

Excellent
Abreviews
Application
Western blot
Loading amount
10 µg
Gel Running Conditions
Reduced Denaturing (4-20%)
Sample
Mouse Cell lysate - whole cell (Oligodendrocyte- GluR2 WT and Null)
Specification
Oligodendrocyte- GluR2 WT and Null
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C

Other product details

Incubation time
1 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: 5% BSA
Dilution
1/2000

Secondary antibody

Name
Non-Abcam antibody was used: Licor Goat Anti Rabbit IR 680
Host species: Goat
Clonality: Polyclonal
Conjugation: IRDye­ 680
Dilution
1/20000

Detection

Detection method
Licor IR
Bands
Specific: 100 kDa
Exposure
30 second(s)
Negative control
Lane 2 = GluR2 null animal
Positive control
We possess a GluR2 knockout animal. I have generated Mouse Oligodendrocyte Progenitor Cells (OPC's) from WT and Null animals and have also included Rat Brain lysate. Lane1 = GluR2 WT Lane2 = GluR2 Null Lane3 = Rat Brain Lysate

Ms. Katherine Given

Verified customer

Submitted Mar 17 2015

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