Recombinant
RabMAb

Recombinant Anti-Ionotropic Glutamate receptor 2 antibody [EPR5032] - BSA and Azide free (ab240061)

Overview

  • Product name
    Anti-Ionotropic Glutamate receptor 2 antibody [EPR5032] - BSA and Azide free
    See all Ionotropic Glutamate receptor 2 primary antibodies
  • Description
    Rabbit monoclonal [EPR5032] to Ionotropic Glutamate receptor 2 - BSA and Azide free
  • Host species
    Rabbit
  • Tested applications
    Suitable for: Flow Cyt, ICC/IF, IP, WBmore details
    Unsuitable for: IHC-P
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Ionotropic Glutamate receptor 2 aa 150-250. The exact sequence is proprietary.

  • General notes

    The formulation and the concentration of this product is compatible for metal-conjugation for mass cytometry (CyTOF®).

    ab240061 is a PBS-only buffer format of ab133477. Please refer to ab133477 for recommended dilutions, protocols, and image data.

     

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab240061 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 98 kDa.
  • Application notes
    Is unsuitable for IHC-P.
  • Target

    • Function
      Ionotropic glutamate receptor. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist. In the presence of CACNG4 or CACNG7 or CACNG8, shows resensitization which is characterized by a delayed accumulation of current flux upon continued application of glutamate.
    • Sequence similarities
      Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. GRIA2 subfamily.
    • Post-translational
      modifications
      Palmitoylated. Depalmitoylated upon glutamate stimulation. Cys-610 palmitoylation leads to Golgi retention and decreased cell surface expression. In contrast, Cys-836 palmitoylation does not affect cell surface expression but regulates stimulation-dependent endocytosis.
    • Cellular localization
      Cell membrane. Endoplasmic reticulum membrane. Cell junction > synapse > postsynaptic cell membrane. Interaction with CACNG2, CNIH2 and CNIH3 promotes cell surface expression.
    • Information by UniProt
    • Database links
    • Alternative names
      • AMPA 2 antibody
      • AMPA selective glutamate receptor 2 antibody
      • AMPA-selective glutamate receptor 2 antibody
      • AMPA2 antibody
      • GluA2 antibody
      • GLUR 2 antibody
      • GLUR B antibody
      • GluR K2 antibody
      • GluR-2 antibody
      • GluR-B antibody
      • GluR-K2 antibody
      • GLUR2 antibody
      • GLURB antibody
      • Glutamate receptor 2 antibody
      • Glutamate receptor ionotropic AMPA 2 antibody
      • Glutamate receptor ionotropic antibody
      • Gria2 antibody
      • GRIA2_HUMAN antibody
      • HBGR2 antibody
      see all

    Images

    • Flow cytometry analysis of SH-SY5Y ( Human neuroblastoma epithelial cell) cells labeling Ionotropic Glutamate receptor 2 (red) with ab133477 at a 1/200 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (Black) (ab172730). Blue (unlabeled control) - Cell without incubation with primary antibody and secondary antibody (Blue).

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133477).

    • ab133477 staining GluR2 in SK-N-SH cells treated with VU 0360223 (ab120803), by ICC/IF. Decrease of GluR2 expression correlates with increased concentration of VU 0360223, as described in literature.
      The cells were incubated at 37°C for 6h in media containing different concentrations of ab120803 (VU 0360223) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab133477 (1/200 dilution was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133477).

    References

    ab240061 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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