Key features and details
- Rabbit polyclonal to Ionotropic Glutamate receptor 2 (phospho S880)
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-Ionotropic Glutamate receptor 2 (phospho S880) antibody
See all Ionotropic Glutamate receptor 2 primary antibodies
DescriptionRabbit polyclonal to Ionotropic Glutamate receptor 2 (phospho S880)
SpecificityDetects endogenous levels of Glutamate receptor 2 only when phosphorylated at serine 880.
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Mouse, Human
Synthetic phosphopeptide derived from human Glutamate receptor 2 around the phosphorylation site of serine 880 (I-E-SP-V-K).
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab52180 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use at an assay dependent concentration.|
|WB||1/500 - 1/1000. Detects a band of approximately 99 kDa (predicted molecular weight: 99 kDa).|
FunctionIonotropic glutamate receptor. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist. In the presence of CACNG4 or CACNG7 or CACNG8, shows resensitization which is characterized by a delayed accumulation of current flux upon continued application of glutamate.
Sequence similaritiesBelongs to the glutamate-gated ion channel (TC 1.A.10.1) family. GRIA2 subfamily.
modificationsPalmitoylated. Depalmitoylated upon glutamate stimulation. Cys-610 palmitoylation leads to Golgi retention and decreased cell surface expression. In contrast, Cys-836 palmitoylation does not affect cell surface expression but regulates stimulation-dependent endocytosis.
Cellular localizationCell membrane. Endoplasmic reticulum membrane. Cell junction > synapse > postsynaptic cell membrane. Interaction with CACNG2, CNIH2 and CNIH3 promotes cell surface expression.
- Information by UniProt
- AMPA 2 antibody
- AMPA selective glutamate receptor 2 antibody
- AMPA-selective glutamate receptor 2 antibody
All lanes : Anti-Ionotropic Glutamate receptor 2 (phospho S880) antibody (ab52180) at 1/500 dilution
Lane 1 : extracts from mouse brain
Lane 2 : extracts from mouse brain with blocking phosphopeptide
Predicted band size: 99 kDa
Ab52180 at 1/50 dilution staining Human brain tissue, with and without blocking peptide; paraffin embedded.
ab52180 has been referenced in 13 publications.
- Lauretti E et al. Extra virgin olive oil improves synaptic activity, short-term plasticity, memory, and neuropathology in a tauopathy model. Aging Cell 19:e13076 (2020). PubMed: 31762202
- Taoro-Gonzalez L et al. Extracellular cGMP Reverses Altered Membrane Expression of AMPA Receptors in Hippocampus of Hyperammonemic Rats: Underlying Mechanisms. Mol Neurobiol 56:4428-4439 (2019). PubMed: 30328550
- Han K et al. Islet-cell autoantigen 69 mediates the antihyperalgesic effects of electroacupuncture on inflammatory pain by regulating spinal glutamate receptor subunit 2 phosphorylation through protein interacting with C-kinase 1 in mice. Pain 160:712-723 (2019). PubMed: 30699097
- Chopra R et al. Protein kinase C activity is a protective modifier of Purkinje neuron degeneration in cerebellar ataxia. Hum Mol Genet 27:1396-1410 (2018). PubMed: 29432535
- Khlghatyan J et al. Mental Illnesses-Associated Fxr1 and Its Negative Regulator Gsk3ß Are Modulators of Anxiety and Glutamatergic Neurotransmission. Front Mol Neurosci 11:119 (2018). PubMed: 29706865