Key features and details
- Rabbit polyclonal to IOP2
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-IOP2 antibody
See all IOP2 primary antibodies
DescriptionRabbit polyclonal to IOP2
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Cow
Recombinant fragment corresponding to Human IOP2 aa 1-239.
Database link: Q9UHQ1)
- A549, H1299, HCT116, MCF7 and Mouse muscle whole cell lysates; Mouse C2C12 xenograft tissue.
Previously labelled as NARF.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab153911 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 51 kDa.|
|IHC-P||1/100 - 1/1000.|
RelevanceSeveral proteins have been found to be prenylated and methylated at their carboxyl-terminal ends. Prenylation was initially believed to be important only for membrane attachment. However, another role for prenylation appears to be its importance in protein-protein interactions. The only nuclear proteins known to be prenylated in mammalian cells are prelamin A- and B-type lamins. Prelamin A is farnesylated and carboxymethylated on the cysteine residue of a carboxyl-terminal CaaX motif. This post-translationally modified cysteine residue is removed from prelamin A when it is endoproteolytically processed into mature lamin A. The protein encoded by this gene binds to the prenylated prelamin A carboxyl-terminal tail domain. It may be a component of a prelamin A endoprotease complex. The encoded protein is located in the nucleus, where it partially colocalizes with the nuclear lamina. It shares limited sequence similarity with iron-only bacterial hydrogenases. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene, including one with a novel exon that is generated by RNA editing.
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All lanes : Anti-IOP2 antibody (ab153911) at 1/1000 dilution
Lane 1 : A549 whole cell lysate
Lane 2 : H1299 whole cell lysate
Lane 3 : HCT116 whole cell lysate
Lane 4 : MCF7 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 51 kDa
7.5% SDS PAGE
Anti-IOP2 antibody (ab153911) at 1/1000 dilution + Mouse muscle whole cell lysate at 50 µg
Predicted band size: 51 kDa
7.5% SDS PAGE
Immunohistochemical analysis of paraffin-embedded Mouse C2C12 xenograft tissue labeling IOP2 with ab153911 at 1/500 dilution.
ab153911 has not yet been referenced specifically in any publications.