Anti-IP10 antibody [6D4] (Biotin) (ab8099)

Mouse monoclonal IP10 antibody [6D4] conjugated to Biotin. Validated in WB, ELISA, Flow Cyt and tested in Human. Independently reviewed in 2 review(s).

Overview

  • Product name

    Anti-IP10 antibody [6D4] (Biotin)
    See all IP10 primary antibodies
  • Description

    Mouse monoclonal [6D4] to IP10 (Biotin)
  • Host species

    Mouse
  • Conjugation

    Biotin
  • Specificity

    The antibody reacts specifically with Human IP-10.
  • Tested applications

    Suitable for: ELISA, Flow Cyt, WBmore details
  • Species reactivity

    Reacts with: Human

Properties

Applications

Our Abpromise guarantee covers the use of ab8099 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.

ab97679 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

 

WB Use at an assay dependent concentration.

Target

  • Function

    Chemotactic for monocytes and T-lymphocytes. Binds to CXCR3.
  • Sequence similarities

    Belongs to the intercrine alpha (chemokine CxC) family.
  • Post-translational
    modifications

    CXCL10(1-73) is produced by proteolytic cleavage after secretion from keratinocytes.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Database links

  • Alternative names

    • Interferon gamma induced factor MOB1, mouse, homolog of antibody
    • Interferon gamma induced protein 10 antibody
    • 10 kDa interferon gamma induced protein antibody
    • 10 kDa interferon gamma-induced protein antibody
    • C X C motif chemokine 10 antibody
    • C7 antibody
    • Chemokine (C X C motif) ligand 10 antibody
    • Chemokine CXC motif ligand 10 antibody
    • Crg 2 antibody
    • CRG2 antibody
    • CXCL10 antibody
    • CXCL10(1-73) antibody
    • CXL10_HUMAN antibody
    • Gamma IP10 antibody
    • Gamma-IP10 antibody
    • gIP 10 antibody
    • GIP10 antibody
    • IFI10 antibody
    • INP 10 antibody
    • INP10 antibody
    • Interferon activated gene 10 antibody
    • Interferon activated gene 10 antibody
    • Interferon gamma induced cell line antibody
    • Interferon inducible cytokine IP 10 antibody
    • Interferon inducible cytokine IP10 antibody
    • IP 10 antibody
    • IP-10 antibody
    • Mob 1 antibody
    • MOB1 antibody
    • Protein 10 from interferon (gamma) induced cell line antibody
    • SCYB10 antibody
    • Small inducible cytokine B10 antibody
    • Small inducible cytokine B10 precursor antibody
    • Small inducible cytokine subfamily B (Cys X Cys) member 10 antibody
    • Small inducible cytokine subfamily B CXC member 10 antibody
    • Small inducible cytokine subfamily B, member 10 antibody
    • Small-inducible cytokine B10 antibody
    see all

Images

  • Detection of IP-10 in HUVEC cells. Red, black and blue line represent the isotype control, cells only and ab8099 at 20 μg/ml, respectively.

References

ab8099 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Application
Western blot
Sample
Human Purified protein
Gel Running Conditions
Non-reduced Non-Denaturing (Native)
Loading amount
0.2 µg
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1%

Abcam user community

Verified customer

Submitted Sep 20 2018

Application
ELISA
Sample
Human Serum (Blood)
Specification
Blood
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1µg/mL · Temperature: 25°C
Type
Sandwich (Detection)

Abcam user community

Verified customer

Submitted Aug 02 2018

Answer

Thank you for contacting us.

We got the information for mouse cross-reactivity from one of the references (PMID: 16721359) for this antibody, but haven't tested it ourselves. However, as both Flow Cytometry as well as mouse are listed as tested, you are 100% covered by our Abpromise guarantee.

The biotinylated version of this antibody in our catalog is simply not as well described as the non-modified version, but as they are derived from the same clone, the modified antibody probably works in mouse as well..

For your information, here is the note regarding theprotocol for intracellular staining: "activated plasmacytoid dendritic cells were fixed with 2% paraformaldehyde and permeabilized with 0.5% saponin. All steps were performed in presence of 0.5 M EDTA to avoid cell aggregation".


I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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