Product nameAnti-IP10 antibody
See all IP10 primary antibodies
DescriptionRabbit polyclonal to IP10
SpecificityThis antibody does not cross-react with murine CXCL9 and CXCL11.
Tested applicationsSuitable for: WB, ELISA, Neutralising, ICC/IFmore details
Species reactivityReacts with: Mouse
Highly pure (>98%) recombinant mIP-10 ( mouse Interferon Inducible Protein 10)
- Recombinant mouse IP10 protein (ab9944) can be used as a positive control in WB.
FormLyophilised:Reconstitute with 200µl of sterile water.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Storage bufferPBS, pH 7.4, no preservative, sterile filtered
Concentration information loading...
PurityImmunogen affinity purified
Light chain typeunknown
Our Abpromise guarantee covers the use of ab9938 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration. To detect mIP-10 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mIP-10 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.|
|ELISA||Use at an assay dependent concentration. To detect mIP-10 by direct ELISA (using 100µl/well antibody solution) a concentration of at least 0.5µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant mIP-10.Western Blot:To detect mIP-10|
|Neutralising||Use at an assay dependent concentration. To yield one-half maximal inhibition [ND50] of the biological activity of mIP-10 (15.00 ng/ml), a concentration of 0.5-0.9 µg/ml of this antibody is required.|
|ICC/IF||Use at an assay dependent concentration.|
FunctionChemotactic for monocytes and T-lymphocytes. Binds to CXCR3.
Sequence similaritiesBelongs to the intercrine alpha (chemokine CxC) family.
modificationsCXCL10(1-73) is produced by proteolytic cleavage after secretion from keratinocytes.
- Information by UniProt
- Interferon gamma induced factor MOB1, mouse, homolog of antibody
- Interferon gamma induced protein 10 antibody
- 10 kDa interferon gamma induced protein antibody
Western blot performed with ab9938 at 0.2 ug/ml
Lane 1: MultiMark MultiColor Standard (Invitrogen)
Lane 2: 250 ng Murine IP-10
Lane 3: 125 ng Murine IP-10
Lane 4: 62.5 ng Murine IP-10
Lane 5: 31.25 ng Murine IP-10
Lane 6: 15.625 ng Murine IP-10
Lane 7: 7.8 ng Murine IP-10
Lane 8: 3.9 ng Murine IP-10
Lane 9: 1.95 ng Murine IP-10
Lane 10: 0.975 ng Murine IP-10
Lane 11: 0.4875 ng Murine IP-10
Lane 12 0.24 ng Murine IP-10
Staining of colchicine injected mouse brain (including the hippocampus region) tissue. The primary antibody was incubated at 1.0 ug/ml overnight at 4°C. This was followed by a peroxidase conjugated secondary antibody and then a fluorescein reagent. Optimal concentrations and conditions may vary.
Staining in colchicine injected mouse brain (including the hippocampus region) tissue. The primary antibody was incubated at 1.0 ug/ml overnight at 4°C. This was followed by a peroxidase conjugated secondary antibody and then a fluorescein reagent. Optimal concentrations and conditions may vary.
This product has been referenced in:
- Fletcher JS et al. Cxcr3-expressing leukocytes are necessary for neurofibroma formation in mice. JCI Insight 4:N/A (2019). Read more (PubMed: 30728335) »
- Zhu X et al. A distinct brain pathway links viral RNA exposure to sickness behavior. Sci Rep 6:29885 (2016). Read more (PubMed: 27435819) »