Product nameAnti-IP10 antibody (Biotin)
See all IP10 primary antibodies
DescriptionRabbit polyclonal to IP10 (Biotin)
Tested applicationsSuitable for: WB, ELISAmore details
Species reactivityReacts with: Human
- WB: Recombinant human IP10.
FormLyophilised:Reconstitute in sterile PBS with 0.1% BSA to 0.1 - 1.0 mg/ml.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark.
Storage bufferpH: 7.20
Constituents: PBS, 0.1% BSA
Concentration information loading...
Our Abpromise guarantee covers the use of ab271283 in the following tested applications.
|WB||Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 10 kDa.
Used in conjunction with compatible secondary reagents the detection limit for recombinant hIP-10 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
|ELISA||Use a concentration of 0.25 - 1 µg/ml.
Human IP10 was detected by sandwich ELISA (using 100μl/well) using a concentration of 0.25-1.0 μg/ml of ab271283. This antibody in conjunction with an appropriate capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant hIP10 protein.
FunctionChemotactic for monocytes and T-lymphocytes. Binds to CXCR3.
Sequence similaritiesBelongs to the intercrine alpha (chemokine CxC) family.
modificationsCXCL10(1-73) is produced by proteolytic cleavage after secretion from keratinocytes.
- Information by UniProt
- Interferon gamma induced factor MOB1, mouse, homolog of antibody
- Interferon gamma induced protein 10 antibody
- 10 kDa interferon gamma induced protein antibody
Lane 1: Marker.
Lane 2: Recombinant hIP10 at 250ng/lane.
Lane 3: Recombinant hIP10 at 125ng/lane.
Lane 4: Recombinant hIP10 at 62.5ng/lane.
Lane 5: Recombinant hIP10 at 31.25ng/lane.
Lane 6: Recombinant hIP10 at 15.625ng/lane.
Lane 7: Recombinant hIP10 at 7.8ng/lane.
Lane 8: Recombinant hIP10 at 3.9ng/lane.
Lane 9: Recombinant hIP10 at 1.95ng/lane.
Lane 10: Recombinant hIP10 at 0.975ng/lane.
Lane 11: Recombinant hIP10 at 0.4875ng/lane.
Lane 12: Recombinant hIP10 at 0.24ng/lane.
Human IP10 was detected by sandwich ELISA (using 100 µl/well) using a concentration of 0.25-1.0 µg/ml of ab271283. This antibody in conjunction with an appropriate capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant IP10 protein.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab271283 has not yet been referenced specifically in any publications.